Detailed information NTG0001393

Experimentally validated

Overview

• Name: tfoX
• Type: Regulator
• Locus tag: DSB67_RS06270
• Genome accession: NZ_CP030788
• Coordinates: 1308598..1309185 (+)
• Length: 195 a.a.
• NCBI ID: WP_005428539.1
• Uniprot ID: A0AAQ2XWF3
• Organism: Vibrio campbellii strain DS40M4
• Function: positive regulator of competence gene Competence regulation

Function

TfoX expression in Vibrio campbellii strains DS40M4 and NBRC 15631 enables high natural transformation frequencies. Similar to V. cholerae, expression of both QstR and TfoX is necessary for transformation in DS40M4.

Genomic Context

Location: 1303598..1314185

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DSB67_RS06240 (DSB67_06240)	panP	1303804..1305450 (-)	1647	WP_010646008.1	pyridoxal-dependent aspartate 1-decarboxylase PanP	-
DSB67_RS06245 (DSB67_06245)	-	1305572..1306084 (-)	513	WP_010646007.1	hypothetical protein	-
DSB67_RS06250 (DSB67_06250)	-	1306294..1307199 (-)	906	WP_010646006.1	lysine exporter LysO family protein	-
DSB67_RS06255 (DSB67_06255)	-	1307307..1308227 (-)	921	WP_010646005.1	HDOD domain-containing protein	-
DSB67_RS06270 (DSB67_06270)	tfoX	1308598..1309185 (+)	588	WP_005428539.1	TfoX/Sxy family DNA transformation protein	Regulator
DSB67_RS06275 (DSB67_06275)	-	1309400..1309948 (-)	549	WP_010646003.1	hypothetical protein	-
DSB67_RS06280 (DSB67_06280)	-	1310156..1310653 (-)	498	WP_010646001.1	response regulator	-
DSB67_RS06285 (DSB67_06285)	-	1310650..1312590 (-)	1941	WP_029388695.1	ATP-binding protein	-
DSB67_RS06290 (DSB67_06290)	-	1312776..1314038 (+)	1263	WP_029388694.1	DUF945 family protein	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
tfoX	pilB	positive effect
tfoX	dprA	positive effect
tfoX	pilC	positive effect
tfoX	pilD	positive effect
tfoX	pilM	positive effect
tfoX	pilO	positive effect
tfoX	comM	positive effect
tfoX	pilA	positive effect
tfoX	pilQ	positive effect
tfoX	comEC	positive effect
tfoX	comEA	positive effect
tfoX	pilP	positive effect
tfoX	comF	positive effect
tfoX	pilN	positive effect

Sequence

Protein

• Length: 195 a.a.
• Molecular weight: 22337.57 Da
• Isoelectric Point: 6.4656

>NTDB_id=1393 DSB67_RS06270 WP_005428539.1 1308598..1309185(+) (tfoX) [Vibrio campbellii strain DS40M4]
MDMTDQAFFNYVSNFSEYQKRSMFGGIGLFSNDAMFALVSNDCCYLRGGNGLDEEFTQLNCEKYKHVKKQTTATVNYYDV
TELFDSGFAGLDELLQKSINCSIKERKYQKSSASRRLRDLPNMQLTLERMVKKAGIDDVDTFLELGPVEVFNKVRVAYGS
DVDVKLLWKFAGAIDGIHWKLIQEPRKKQLLAMCE

Nucleotide

• Length: 588 bp

>NTDB_id=1393 DSB67_RS06270 WP_005428539.1 1308598..1309185(+) (tfoX) [Vibrio campbellii strain DS40M4]
ATGGACATGACAGACCAAGCTTTTTTTAACTATGTAAGCAATTTTAGTGAGTACCAAAAACGTTCAATGTTCGGCGGTAT
TGGTTTATTCAGCAACGACGCAATGTTTGCTCTGGTAAGTAACGACTGCTGTTACTTGCGCGGTGGCAATGGCCTTGATG
AAGAGTTTACTCAACTAAACTGTGAAAAATATAAGCACGTCAAAAAACAAACGACGGCAACGGTAAATTATTACGATGTA
ACAGAATTGTTCGATTCCGGTTTTGCTGGGTTGGATGAGCTGCTACAAAAATCAATTAATTGTTCTATCAAGGAACGCAA
ATACCAAAAGTCTTCAGCCAGTAGACGTTTGCGTGATCTACCGAATATGCAGCTAACGCTAGAGCGTATGGTAAAGAAAG
CGGGTATTGATGACGTAGACACGTTTTTGGAGCTTGGCCCAGTAGAAGTCTTCAACAAAGTAAGAGTGGCATACGGAAGT
GATGTAGACGTTAAGCTGCTTTGGAAGTTTGCTGGTGCGATTGATGGTATCCATTGGAAACTGATCCAAGAACCACGCAA
GAAGCAGCTACTGGCTATGTGTGAATAA

Domains

Predicted by InterproScan.

• TfoX N-terminal domain (TfoX_N) (11-99)

• TfoX C-terminal domain (TfoX_C) (114-192)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
tfoX	Vibrio parahaemolyticus RIMD 2210633	91.795	100	0.918
tfoX	Vibrio cholerae strain A1552	69.43	98.974	0.687
tfoX	Vibrio cholerae O1 biovar El Tor strain E7946	69.43	98.974	0.687
tfoX	Vibrio cholerae O1 biovar El Tor strain P27459	69.43	98.974	0.687
tfoX	Vibrio cholerae O1 biovar El Tor strain DRC-193A	69.43	98.974	0.687
tfoX	Vibrio cholerae O1 biovar El Tor str. N16961	69.43	98.974	0.687
tfoX	Aliivibrio fischeri ES114	59.162	97.949	0.579

References

• [1]: Chelsea A Simpson et al. (2019) Diversity in Natural Transformation Frequencies and Regulation across Vibrio Species. MBio 10(6):e02788-19. [PMID: 31848285]