Detailed information    

experimental Experimentally validated

Overview

Name   tfoX   Type   Regulator
Locus tag   VF_RS04680 Genome accession   NC_006840
Coordinates   986000..986629 (+) Length   209 a.a.
NCBI ID   WP_011261559.1    Uniprot ID   -
Organism   Aliivibrio fischeri ES114     
Function   master regulator of natural competence   
Competence regulation

Function

Chitohexaose-independent transformation by V. fischeri was observed when tfoX was present in multicopy. The second tfoX paralogue, designated tfoY, is also required for efficient transformation in V. fischeri, but is not functionally identical to tfoX.


Genomic Context

Location: 981000..991629
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VF_RS04660 (VF0892) panP 981246..982889 (-) 1644 WP_011261555.1 pyridoxal-dependent aspartate 1-decarboxylase PanP -
  VF_RS04665 (VF0893) - 983064..983576 (-) 513 WP_011261556.1 hypothetical protein -
  VF_RS04670 (VF0894) - 983713..984612 (-) 900 WP_011261557.1 lysine exporter LysO family protein -
  VF_RS04675 (VF0895) - 984703..985626 (-) 924 WP_011261558.1 HDOD domain-containing protein -
  VF_RS04680 (VF0896) tfoX 986000..986629 (+) 630 WP_011261559.1 DNA transformation protein TfoX Regulator
  VF_RS04685 (VF0897) - 986721..987269 (-) 549 WP_005418478.1 hypothetical protein -
  VF_RS04690 (VF0898) - 987545..988846 (+) 1302 WP_011261560.1 DUF945 family protein -
  VF_RS04695 (VF0899) serC 989044..990126 (+) 1083 WP_011261561.1 3-phosphoserine/phosphohydroxythreonine transaminase -

Sequence

Protein


Download         Length: 209 a.a.        Molecular weight: 23868.52 Da        Isoelectric Point: 8.8400

>NTDB_id=1301 VF_RS04680 WP_011261559.1 986000..986629(+) (tfoX) [Aliivibrio fischeri ES114]
MTGTGDVNLKIKEIEMSHLEERFFNFVKKLGRFNKRSMFGGVGLFNEDAMFSLVTDGRLYVRGGGEVDARFEELGCERFK
HVKKTTIATVNYFDVTELFEKKPASLLSIVQEAMENSRLEREFKKSKENRRLRDLPNMQLTLERMVKKAGVPDVDSFLEI
GAVDVFKKVNHTYGGDVDVKLLWKFAGAESGVHWTLLQEPAKQALLQQV

Nucleotide


Download         Length: 630 bp        

>NTDB_id=1301 VF_RS04680 WP_011261559.1 986000..986629(+) (tfoX) [Aliivibrio fischeri ES114]
ATGACAGGGACGGGGGATGTTAATTTAAAGATCAAGGAGATCGAGATGAGTCATCTAGAAGAGCGCTTTTTTAACTTTGT
AAAAAAACTGGGAAGATTTAATAAACGTTCAATGTTTGGTGGTGTTGGATTGTTTAATGAAGATGCTATGTTCTCACTTG
TTACTGACGGACGATTATATGTTCGTGGTGGTGGTGAAGTAGATGCTCGGTTTGAAGAGTTAGGTTGTGAGCGTTTCAAG
CATGTTAAGAAGACGACAATTGCTACTGTAAATTATTTTGATGTTACTGAGTTATTTGAAAAGAAACCGGCTTCTCTTCT
TTCTATTGTTCAAGAAGCTATGGAAAATTCTCGTTTAGAAAGAGAATTTAAAAAATCAAAAGAAAACCGCCGTTTACGTG
ACTTACCAAATATGCAACTGACATTAGAAAGAATGGTCAAAAAAGCAGGAGTTCCTGATGTTGATTCATTTCTGGAAATT
GGTGCTGTTGATGTATTTAAAAAAGTGAATCACACCTATGGTGGTGATGTGGATGTTAAGCTACTTTGGAAATTTGCTGG
AGCGGAGTCAGGCGTTCATTGGACTTTATTACAAGAGCCTGCAAAGCAAGCATTATTGCAGCAAGTTTAA

Domains

Predicted by InterproScan.

TfoX N-terminal domain (TfoX_N)

(27-116)

TfoX C-terminal domain (TfoX_C)

(129-208)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  tfoX Vibrio campbellii strain DS40M4

59.162

97.949

0.579
  tfoX Vibrio cholerae strain A1552

59.043

96.41

0.569
  tfoX Vibrio cholerae O1 biovar El Tor strain E7946

59.043

96.41

0.569
  tfoX Vibrio cholerae O1 biovar El Tor strain P27459

59.043

96.41

0.569
  tfoX Vibrio cholerae O1 biovar El Tor strain DRC-193A

59.043

96.41

0.569
  tfoX Vibrio cholerae O1 biovar El Tor str. N16961

59.043

96.41

0.569
  tfoX Vibrio parahaemolyticus RIMD 2210633

57.068

97.949

0.559

Multiple sequence alignment    



References

[1] Amber Pollack-Berti et al. (2010) Natural transformation of Vibrio fischeri requires tfoX and tfoY. Environmental Microbiology 12(8):2302-11. [PMID: 21966921]