Detailed information NTG0001289

Experimentally validated

Overview

• Name: tfoX
• Type: Regulator
• Locus tag: VP_RS06025
• Genome accession: NC_004603
• Coordinates: 1309223..1309810 (+)
• Length: 195 a.a.
• NCBI ID: WP_005459593.1
• Uniprot ID: Q87QA8
• Organism: Vibrio parahaemolyticus RIMD 2210633
• Function: master regulator of natural competence Competence regulation

Function

TfoX regulates natural competence via CytR in a chitin-dependent manner. The Δtfox mutant showed 142.3-, 52.8-, 77.9-, 50.1-, and 21.9-fold downregulation in pilA, pilB, comEA, comEC, and qstR, respectively.

Genomic Context

Location: 1304223..1314810

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VP_RS06005 (VP1237)	panP	1304392..1306038 (-)	1647	WP_005483514.1	pyridoxal-dependent aspartate 1-decarboxylase PanP	-
VP_RS06010 (VP1238)	-	1306199..1306711 (-)	513	WP_005483562.1	hypothetical protein	-
VP_RS06015 (VP1239)	-	1306919..1307824 (-)	906	WP_011105795.1	lysine exporter LysO family protein	-
VP_RS06020 (VP1240)	-	1307932..1308852 (-)	921	WP_005483559.1	HDOD domain-containing protein	-
VP_RS06025 (VP1241)	tfoX	1309223..1309810 (+)	588	WP_005459593.1	TfoX/Sxy family DNA transformation protein	Regulator
VP_RS06030 (VP1243)	-	1309984..1310529 (-)	546	WP_005459590.1	hypothetical protein	-
VP_RS06035 (VP1244)	-	1310727..1311224 (-)	498	WP_005483573.1	response regulator	-
VP_RS06040 (VP1245)	-	1311221..1313161 (-)	1941	WP_005489098.1	ATP-binding protein	-
VP_RS06045 (VP1246)	-	1313348..1314613 (+)	1266	WP_005483545.1	DUF945 family protein	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
tfoX	qstR	positive effect
tfoX	comEC	positive effect
tfoX	pilB	positive effect
tfoX	comEA	positive effect
tfoX	pilA	positive effect
cytR	qstR	positive effect
cytR	pilA	positive effect
cytR	comEC	positive effect
cytR	pilB	positive effect
cytR	comEA	positive effect

Sequence

Protein

• Length: 195 a.a.
• Molecular weight: 22459.81 Da
• Isoelectric Point: 7.0628

>NTDB_id=1289 VP_RS06025 WP_005459593.1 1309223..1309810(+) (tfoX) [Vibrio parahaemolyticus RIMD 2210633]
MDMTDQAFFKYVRNFNEYQKRSMFGGIGLFSDDAMFALVSNDCCYLRGGNGLDEEFTLLNCEKYKHVKKQTTATVNYYDV
TDLFESGFTGLDDLLRKSIDCSIKERKYQKSSASKRLRDLPNMQLTLERMVKKAGIDDVETFLELGPVEVFNKVRVAYGN
DVDVKLLWKFAGAIDGVHWKLIQEPRKKQLLALCE

Nucleotide

• Length: 588 bp

>NTDB_id=1289 VP_RS06025 WP_005459593.1 1309223..1309810(+) (tfoX) [Vibrio parahaemolyticus RIMD 2210633]
ATGGACATGACAGACCAAGCTTTTTTTAAATATGTACGCAATTTTAATGAGTATCAGAAACGTTCTATGTTTGGTGGTAT
CGGCTTGTTTAGCGATGACGCCATGTTTGCGCTTGTCAGCAATGATTGCTGTTATTTGCGCGGCGGTAATGGTTTAGACG
AAGAATTTACTCTGCTTAATTGTGAAAAATACAAGCACGTCAAAAAACAGACGACGGCTACTGTAAACTATTATGACGTA
ACCGATTTATTTGAGTCCGGATTTACTGGTCTTGACGATTTGTTACGCAAATCTATTGATTGCTCAATTAAGGAGCGAAA
ATACCAAAAGTCTTCTGCAAGTAAACGTTTGAGAGATCTACCGAACATGCAGCTTACATTAGAGCGCATGGTTAAAAAGG
CGGGTATTGATGATGTTGAAACCTTTTTGGAACTTGGTCCAGTTGAAGTGTTCAACAAAGTAAGAGTTGCTTACGGCAAT
GATGTTGACGTTAAACTGCTGTGGAAGTTTGCTGGTGCCATTGATGGTGTGCACTGGAAACTAATCCAAGAGCCGCGCAA
AAAACAGTTACTGGCACTGTGTGAATAA

Domains

Predicted by InterproScan.

• TfoX N-terminal domain (TfoX_N) (11-101)

• TfoX C-terminal domain (TfoX_C) (114-192)

3D structure

• AlphaFold DB: Q87QA8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
tfoX	Vibrio campbellii strain DS40M4	91.795	100	0.918
tfoX	Vibrio cholerae strain A1552	68.394	98.974	0.677
tfoX	Vibrio cholerae O1 biovar El Tor strain E7946	68.394	98.974	0.677
tfoX	Vibrio cholerae O1 biovar El Tor strain P27459	68.394	98.974	0.677
tfoX	Vibrio cholerae O1 biovar El Tor strain DRC-193A	68.394	98.974	0.677
tfoX	Vibrio cholerae O1 biovar El Tor str. N16961	68.394	98.974	0.677
tfoX	Aliivibrio fischeri ES114	57.068	97.949	0.559

References

• [1]: Anusuya Debnath et al. (2022) Regulators of natural competence in Vibrio parahaemolyticus. Brazilian Journal of Microbiology : [publication of The Brazilian Society For Microbiology] 53(3):1491-1499. [PMID: 35761009]