Detailed information    

experimental Experimentally validated

Overview

Name   comEA   Type   Machinery gene
Locus tag   VP_RS04495 Genome accession   NC_004603
Coordinates   960354..960638 (+) Length   94 a.a.
NCBI ID   WP_021449594.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus RIMD 2210633     
Function   dsDNA binding   
DNA binding and uptake

Function

The ΔtfoX or ΔcytR mutant showed significant downregulation in pilA, pilB, comEA, comEC, and qstR.


Genomic Context

Location: 955354..965638
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VP_RS04485 (VP0920) - 957872..958144 (+) 273 WP_005382341.1 HU family DNA-binding protein -
  VP_RS04490 (VP0921) ppiD 958344..960203 (+) 1860 WP_005482527.1 peptidylprolyl isomerase -
  VP_RS04495 (VP0922) comEA 960354..960638 (+) 285 WP_021449594.1 ComEA family DNA-binding protein Machinery gene
  VP_RS04500 (VP0924) rrtA 960729..961280 (-) 552 WP_005482515.1 rhombosortase -
  VP_RS04505 (VP0923) - 961284..961898 (+) 615 WP_005482520.1 DTW domain-containing protein -
  VP_RS04510 (VP0925) - 961908..963242 (-) 1335 WP_041954079.1 anti-phage deoxyguanosine triphosphatase -
  VP_RS04515 (VP0926) yfbR 963255..963839 (-) 585 WP_005482513.1 5'-deoxynucleotidase -
  VP_RS04520 (VP0927) - 963930..965144 (-) 1215 WP_005456922.1 pyridoxal phosphate-dependent aminotransferase -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  tfoX comEA positive effect
  tfoX qstR positive effect
  cytR qstR positive effect
  tfoX comEC positive effect
  cytR comEC positive effect
  tfoX pilB positive effect
  cytR pilB positive effect
  cytR comEA positive effect
  tfoX pilA positive effect
  cytR pilA positive effect

Sequence

Protein


Download         Length: 94 a.a.        Molecular weight: 10249.91 Da        Isoelectric Point: 5.8425

>NTDB_id=1294 VP_RS04495 WP_021449594.1 960354..960638(+) (comEA) [Vibrio parahaemolyticus RIMD 2210633]
MKWILTLCLVVFAPLSLAADTKADKYEGIEITVNINTASAEEIATMLKGIGEKKAQSIVDYRTEHGPFKTAADLTNVKGI
GEATIKKNEDRILL

Nucleotide


Download         Length: 285 bp        

>NTDB_id=1294 VP_RS04495 WP_021449594.1 960354..960638(+) (comEA) [Vibrio parahaemolyticus RIMD 2210633]
ATGAAATGGATTTTAACCTTGTGTTTAGTGGTGTTCGCACCACTCAGTTTGGCCGCTGATACCAAGGCTGATAAATATGA
AGGAATTGAAATTACGGTCAATATCAATACTGCTTCTGCAGAAGAGATAGCAACGATGCTGAAAGGTATTGGCGAAAAGA
AAGCGCAAAGCATAGTTGACTACCGTACCGAGCACGGCCCATTTAAAACAGCAGCTGATTTAACCAATGTGAAAGGAATT
GGTGAAGCAACCATCAAGAAAAACGAAGACCGCATTCTTCTATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(32-92)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Vibrio campbellii strain DS40M4

77.895

100

0.787
  comEA Vibrio cholerae C6706

62.766

100

0.628
  comEA Vibrio cholerae strain A1552

62.766

100

0.628
  comE1/comEA Haemophilus influenzae Rd KW20

56.452

65.957

0.372
  comEA/comE1 Glaesserella parasuis strain SC1401

56.452

65.957

0.372
  comE Neisseria gonorrhoeae MS11

38.889

95.745

0.372
  comE Neisseria gonorrhoeae MS11

38.889

95.745

0.372
  comE Neisseria gonorrhoeae MS11

38.889

95.745

0.372
  comE Neisseria gonorrhoeae MS11

38.889

95.745

0.372
  comEA Acinetobacter baylyi ADP1

47.222

76.596

0.362

Multiple sequence alignment    



References

[1] Anusuya Debnath et al. (2022) Regulators of natural competence in Vibrio parahaemolyticus. Brazilian Journal of Microbiology : [publication of The Brazilian Society For Microbiology] 53(3):1491-1499. [PMID: 35761009]