Detailed information    

experimental Experimentally validated

Overview

Name   comEA   Type   Machinery gene
Locus tag   ACIAD_RS13855 Genome accession   NC_005966
Coordinates   2990694..2991080 (-) Length   128 a.a.
NCBI ID   WP_004924483.1    Uniprot ID   -
Organism   Acinetobacter baylyi ADP1     
Function   dsDNA binding   
DNA binding and uptake

Function

ComEA binds the incoming DNA thereby supporting the transport of the DNA through the periplasm.


Genomic Context

Location: 2985694..2996080
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIAD_RS13825 (ACIAD3058) - 2985792..2986061 (-) 270 WP_004924499.1 HPr family phosphocarrier protein -
  ACIAD_RS13830 (ACIAD3059) rapZ 2986058..2986909 (-) 852 WP_004924498.1 RNase adapter RapZ -
  ACIAD_RS13835 (ACIAD3060) panC 2986931..2987776 (-) 846 WP_004924494.1 pantoate--beta-alanine ligase -
  ACIAD_RS13840 (ACIAD3061) panB 2987780..2988589 (-) 810 WP_004924492.1 3-methyl-2-oxobutanoate hydroxymethyltransferase -
  ACIAD_RS13845 (ACIAD3062) folK 2988627..2989115 (-) 489 WP_004924489.1 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase -
  ACIAD_RS13850 (ACIAD3063) pcnB 2989112..2990569 (-) 1458 WP_004924487.1 polynucleotide adenylyltransferase PcnB -
  ACIAD_RS13855 (ACIAD3064) comEA 2990694..2991080 (-) 387 WP_004924483.1 ComEA family DNA-binding protein Machinery gene
  ACIAD_RS13860 (ACIAD3065) mazG 2991084..2991851 (-) 768 WP_004924480.1 nucleoside triphosphate pyrophosphohydrolase -
  ACIAD_RS13865 (ACIAD3067) - 2991965..2992747 (+) 783 WP_004924478.1 SDR family oxidoreductase -
  ACIAD_RS13870 (ACIAD3068) - 2992794..2995100 (-) 2307 WP_004924472.1 RelA/SpoT family protein -

Sequence

Protein


Download         Length: 128 a.a.        Molecular weight: 14530.56 Da        Isoelectric Point: 10.4737

>NTDB_id=1014 ACIAD_RS13855 WP_004924483.1 2990694..2991080(-) (comEA) [Acinetobacter baylyi ADP1]
MSIRHFFTFFWATLLTLFSMQIVFAQGFDQQYDAWKTQQKAHDTRLNSSASHHSLSKPTQQSGTGVKVNINSATAKELQQ
LHGVGEKKALAIVEYRQQYGKFASVDDLQKVKGIGPKFIEKNRDRLAI

Nucleotide


Download         Length: 387 bp        

>NTDB_id=1014 ACIAD_RS13855 WP_004924483.1 2990694..2991080(-) (comEA) [Acinetobacter baylyi ADP1]
ATGTCAATACGTCATTTTTTTACATTTTTTTGGGCGACATTGCTTACACTTTTTTCTATGCAAATTGTTTTTGCACAAGG
GTTTGATCAACAATATGATGCTTGGAAAACACAGCAAAAAGCACATGATACCCGTTTAAACTCATCTGCTAGTCATCATT
CCCTGTCTAAACCGACACAGCAATCTGGCACTGGGGTGAAAGTCAATATTAACTCCGCAACGGCCAAAGAATTACAGCAG
TTGCATGGGGTGGGAGAGAAAAAAGCACTCGCCATCGTTGAATATCGTCAGCAGTATGGCAAGTTTGCGTCTGTAGATGA
TCTACAGAAAGTAAAAGGAATTGGCCCTAAATTTATAGAAAAGAACCGAGACCGTTTAGCCATTTAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(65-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Acinetobacter baumannii strain A118

43.511

100

0.445
  comEA Acinetobacter baumannii D1279779

43.511

100

0.445
  Cj0011c Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

57.377

77.215

0.443
  comEA Vibrio campbellii strain DS40M4

48.649

77.895

0.379
  comEA Vibrio parahaemolyticus RIMD 2210633

47.222

76.596

0.362

Multiple sequence alignment    



References

[1] Beate Averhoff et al. (2021) Natural transformation in Gram-negative bacteria thriving in extreme environments: from genes and genomes to proteins, structures and regulation. Extremophiles : Life Under Extreme Conditions 25(5-6):425-436. [PMID: 34542714]
[2] Colleen G Leong et al. (2017) The role of core and accessory type IV pilus genes in natural transformation and twitching motility in the bacterium Acinetobacter baylyi. PloS One 12(8):e0182139. [PMID: 28771515]