Detailed information    

experimental Experimentally validated

Overview

Name   comEA   Type   Machinery gene
Locus tag   ABD1_RS03060 Genome accession   NC_020547
Coordinates   663304..663714 (+) Length   136 a.a.
NCBI ID   WP_043960841.1    Uniprot ID   -
Organism   Acinetobacter baumannii D1279779     
Function   dsDNA binding   
DNA binding and uptake

Function

We examined the 33 knockout mutants by comparing their transformation efficiency with wild-type W068. No knockout strains were affected in growth, but 28 of these mutants were severely or completely impaired in natural transformability. These mutations were in 18 genes related to T4P (pilF, pilQ, tsaP, pilM, pilN, pilO, pilP, pilB, pilC, pilT, pilD, pilE, pilY2, pilY1, pilX, pilW, pilV, and fimU), six genes related to DNA uptake and processing (comEA, comA, comF, priA, dprA, and recA), two genes related to T2SS (xcpV and xcpW), and the crp and tonB2 genes.


Genomic Context

Location: 658304..668714
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABD1_RS03045 (ABD1_05850) - 659293..661599 (+) 2307 WP_000259109.1 RelA/SpoT family protein -
  ABD1_RS03050 (ABD1_05860) - 661644..662417 (-) 774 WP_000682841.1 SDR family oxidoreductase -
  ABD1_RS03055 (ABD1_05870) mazG 662529..663335 (+) 807 WP_000359488.1 nucleoside triphosphate pyrophosphohydrolase -
  ABD1_RS03060 (ABD1_05880) comEA 663304..663714 (+) 411 WP_043960841.1 ComEA family DNA-binding protein Machinery gene
  ABD1_RS03065 (ABD1_05890) pcnB 663839..665302 (+) 1464 WP_001194201.1 polynucleotide adenylyltransferase PcnB -
  ABD1_RS03070 (ABD1_05900) folK 665299..665781 (+) 483 WP_000219213.1 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase -
  ABD1_RS03075 (ABD1_05910) panB 665827..666636 (+) 810 WP_000624763.1 3-methyl-2-oxobutanoate hydroxymethyltransferase -
  ABD1_RS03080 (ABD1_05920) panC 666640..667488 (+) 849 WP_000846362.1 pantoate--beta-alanine ligase -
  ABD1_RS03085 (ABD1_05930) rapZ 667511..668362 (+) 852 WP_000821671.1 RNase adapter RapZ -
  ABD1_RS03090 (ABD1_05940) - 668359..668628 (+) 270 WP_000567533.1 HPr family phosphocarrier protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 15670.97 Da        Isoelectric Point: 10.3495

>NTDB_id=1069 ABD1_RS03060 WP_043960841.1 663304..663714(+) (comEA) [Acinetobacter baumannii D1279779]
MQYSMRFWKNKYFVLIMLCLGVISPSVCAQSFDQNFQEWKVKQQMYDQKLNISKSSHSNSSKISQTKNFNDSTGQIHLNQ
ANVNEFQQLKGIGEKKAQAIVEYRQKNGSFKNIDEIKNVKGIGPAIFEKNKSRLAL

Nucleotide


Download         Length: 411 bp        

>NTDB_id=1069 ABD1_RS03060 WP_043960841.1 663304..663714(+) (comEA) [Acinetobacter baumannii D1279779]
ATGCAATACAGCATGAGATTTTGGAAAAATAAGTATTTTGTTTTAATAATGTTGTGCTTGGGAGTAATTTCACCTTCGGT
TTGCGCTCAATCTTTTGATCAAAACTTTCAAGAATGGAAGGTTAAACAGCAGATGTATGACCAAAAGTTAAACATCTCTA
AGTCTTCTCATTCTAATAGTTCTAAAATTTCTCAAACTAAAAATTTTAATGACTCTACAGGGCAAATTCATTTGAATCAG
GCGAATGTAAATGAGTTTCAGCAGTTAAAAGGTATAGGTGAGAAAAAGGCTCAAGCGATTGTAGAATACAGACAAAAGAA
CGGTAGCTTTAAAAATATTGACGAAATTAAAAACGTTAAAGGAATTGGACCAGCTATTTTTGAAAAAAATAAATCGCGTT
TAGCCTTATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(74-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Acinetobacter baumannii strain A118

96.324

100

0.963
  comEA Acinetobacter baylyi ADP1

43.511

100

0.445
  Cj0011c Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

47.619

79.747

0.38

Multiple sequence alignment    



References

[1] Yuan Hu et al. (2021) Natural Transformation in Acinetobacter baumannii W068: A Genetic Analysis Reveals the Involvements of the CRP, XcpV, XcpW, TsaP, and TonB2. Frontiers in Microbiology 12:738034. [PMID: 35126321]