Detailed information    

experimental Experimentally validated

Overview

Name   comEA   Type   Machinery gene
Locus tag   H0N27_RS14740 Genome accession   NZ_CP059039
Coordinates   3150032..3150442 (-) Length   136 a.a.
NCBI ID   WP_005124713.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain A118     
Function   dsDNA binding   
DNA binding and uptake

Function

Mutant studies with A. baumannii A118 confirmed that pilA, pilQ (secretin, comQ homologue), pilT (retraction ATPase), comEA (DNA-binding protein), comF (pilotin) and dprA (DNA protecting protein) are required for natural transformation


Genomic Context

Location: 3145032..3155442
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H0N27_RS14710 (H0N27_14665) - 3145118..3145387 (-) 270 WP_000567533.1 HPr family phosphocarrier protein -
  H0N27_RS14715 (H0N27_14670) rapZ 3145384..3146235 (-) 852 WP_000821671.1 RNase adapter RapZ -
  H0N27_RS14720 (H0N27_14675) panC 3146258..3147106 (-) 849 WP_000846362.1 pantoate--beta-alanine ligase -
  H0N27_RS14725 (H0N27_14680) panB 3147110..3147919 (-) 810 WP_000624763.1 3-methyl-2-oxobutanoate hydroxymethyltransferase -
  H0N27_RS14730 (H0N27_14685) folK 3147965..3148447 (-) 483 WP_031987940.1 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase -
  H0N27_RS14735 (H0N27_14690) pcnB 3148444..3149907 (-) 1464 WP_031987942.1 polynucleotide adenylyltransferase PcnB -
  H0N27_RS14740 (H0N27_14695) comEA 3150032..3150442 (-) 411 WP_005124713.1 ComEA family DNA-binding protein Machinery gene
  H0N27_RS14745 (H0N27_14700) mazG 3150411..3151217 (-) 807 WP_168726908.1 nucleoside triphosphate pyrophosphohydrolase -
  H0N27_RS14750 (H0N27_14705) - 3151329..3152102 (+) 774 WP_102759853.1 SDR family oxidoreductase -
  H0N27_RS14755 (H0N27_14710) - 3152147..3154453 (-) 2307 WP_000259109.1 RelA/SpoT family protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  hns comEA negative effect
  hns pilQ negative effect
  hns pilT negative effect
  hns comA/comEC negative effect
  hns comF negative effect
  hns pilA negative effect
  pilR pilA positive effect
  hns dprA negative effect
  pilS pilR positive effect

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 15626.92 Da        Isoelectric Point: 10.3495

>NTDB_id=1041 H0N27_RS14740 WP_005124713.1 3150032..3150442(-) (comEA) [Acinetobacter baumannii strain A118]
MQYSMRFWKNKYFLLIMLCLGVISPSVCAQSFDQNFQEWKAKQQMYDQKLNITKSSHSNGSKISQTKNFNDSTGQIHLNQ
ANVNEFQQLKGVGEKKAQAIVEYRQKNGSFKNIDEIKNVKGIGPAIFEKNKSRLAL

Nucleotide


Download         Length: 411 bp        

>NTDB_id=1041 H0N27_RS14740 WP_005124713.1 3150032..3150442(-) (comEA) [Acinetobacter baumannii strain A118]
ATGCAATACAGCATGAGATTTTGGAAAAATAAGTATTTTCTTTTAATAATGTTGTGCTTGGGAGTAATTTCACCTTCGGT
TTGCGCTCAATCTTTTGATCAAAACTTTCAAGAATGGAAGGCTAAACAGCAGATGTATGACCAAAAGTTAAACATCACTA
AGTCTTCTCATTCTAATGGTTCTAAAATTTCTCAAACTAAAAATTTTAATGACTCTACAGGGCAAATTCATTTGAATCAG
GCGAATGTAAATGAGTTTCAGCAATTAAAAGGTGTAGGTGAGAAAAAGGCTCAAGCGATTGTAGAATACAGGCAAAAGAA
CGGTAGTTTTAAAAATATTGACGAAATTAAAAACGTTAAAGGAATTGGACCAGCTATTTTTGAAAAAAATAAATCGCGTT
TAGCCTTATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(74-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Acinetobacter baumannii D1279779

96.324

100

0.963
  comEA Acinetobacter baylyi ADP1

43.511

100

0.445
  comEA Vibrio campbellii strain DS40M4

32.787

100

0.421
  Cj0011c Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

46.032

79.747

0.367

Multiple sequence alignment    



References

[1] Nina Vesel et al. (2021) Pilus Production in Acinetobacter baumannii Is Growth Phase Dependent and Essential for Natural Transformation. Journal of Bacteriology 203(8):e00034-21. [PMID: 33495250]
[2] Casin Le et al. (2021) Involvement of the Histone-Like Nucleoid Structuring Protein (H-NS) in Acinetobacter baumannii's Natural Transformation. Pathogens (Basel, Switzerland) 10(9):1083. [PMID: 34578115]