Detailed information NTG0001083

Experimentally validated

Overview

• Name: comA/comEC
• Type: Machinery gene
• Locus tag: H0N27_RS04190
• Genome accession: NZ_CP059039
• Coordinates: 905987..908419 (+)
• Length: 810 a.a.
• NCBI ID: WP_168726476.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii strain A118
• Function: ssDNA transport through the inner membrane DNA binding and uptake

Function

After degradation of one strand, which, in Gram-negative bacteria, is attributed to an unidentified nuclease or a periplasmic extension of the ComEC protein, the single-stranded DNA translocates across the inner membrane through the ComEC channel aided by the ComF protein.

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Prophage	905216..913096	905987..908419	within	0

Gene organization within MGE regions

Location: 905216..913096

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H0N27_RS04185 (H0N27_04185)	lolD	905216..905902 (+)	687	WP_032058272.1	lipoprotein-releasing ABC transporter ATP-binding protein LolD	-
H0N27_RS04190 (H0N27_04190)	comA/comEC	905987..908419 (+)	2433	WP_168726476.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
H0N27_RS04195 (H0N27_04195)	-	908372..909355 (-)	984	WP_001984639.1	lysophospholipid acyltransferase family protein	-
H0N27_RS04200 (H0N27_04200)	sppA	909492..910508 (+)	1017	WP_001286615.1	signal peptide peptidase SppA	-
H0N27_RS04205 (H0N27_04205)	-	910527..911336 (+)	810	WP_000114071.1	alpha/beta fold hydrolase	-
H0N27_RS04210 (H0N27_04210)	purN	911400..912029 (-)	630	WP_098729345.1	phosphoribosylglycinamide formyltransferase	-
H0N27_RS04215 (H0N27_04215)	purM	912026..913096 (-)	1071	WP_000071984.1	phosphoribosylformylglycinamidine cyclo-ligase	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
hns	comA/comEC	negative effect
hns	pilQ	negative effect
hns	pilT	negative effect
hns	comF	negative effect
hns	pilA	negative effect
pilR	pilA	positive effect
hns	dprA	negative effect
hns	comEA	negative effect
pilS	pilR	positive effect

Sequence

Protein

• Length: 810 a.a.
• Molecular weight: 92495.87 Da
• Isoelectric Point: 8.8556

>NTDB_id=1083 H0N27_RS04190 WP_168726476.1 905987..908419(+) (comA/comEC) [Acinetobacter baumannii strain A118]
MFKIILLGWIGGIALMGIDFPLIMQYEKVGEALLLLAFIFYLYKRPMFVDRPFLKAVFCLLCTTSLFMVGYHYAEKALIE
RLEQRETDTRNLDIIVYINRLSEEKDNKVQQTAQVLNLSKEPVNWLLYLKSNNQNLLKNNQNLELGHYYRISGKTRPAHS
YATPGSFDQEKWFIQRNIMSGFNVRYIEPLSLDEIYRLGYQQHLKEQQSFSSSFRLNIEKLRLTFRQILNSSSLQQKGLI
LALLTGDESLLSDETQLQFKQLGISHLLAISGPHVFIFAIMLSWACHQFISRYYPKIYLWKPKQILMAVPCCLGVLIYTA
FVGFEIPALRTLLSTLIFIGFLLLKQPIKPFTLLVYSASLLLLMDPFSVLSAGFWLSYGACFILIRIYQTIAQLPEQHFL
SLSSKMIFMSKVLIESQGKIFIALSPLTLLFFQRISWVAPLTNIIAVPIVGSVIVPLNIIAACAWFVVKPFGNMLFHFND
MLLSILLSCLGLLEKLSLPLQGISLTPLSLLAISCAIIILFLPKGILPKTWGILCCLPLVMMNKTSQQIQLNILDVGQGQ
AIFLQHPQQNWLIDTGGSYDEKIFSIGQNVVVPFLRQQGVRQLDHVVLSHLDQDHSGAFPIIQQEIPVKQLISNEQLPND
LKQPFQYCHQGQQWHYSELDIQILWPKEKDLAFVASNQNQYSCVVYLQFKKVGGYQNFLIMGDAGWEAEYELLKDYPNLK
IDVLVLGHHGSKHSSAYDFLATLKPKLAIASAGFDNRYGHPSQQVIARLKALHIPLKSTLEQGTLSFVLENHKLVLHDRR
LDRLWLSRGF

Nucleotide

• Length: 2433 bp

>NTDB_id=1083 H0N27_RS04190 WP_168726476.1 905987..908419(+) (comA/comEC) [Acinetobacter baumannii strain A118]
ATGTTTAAGATTATTCTATTGGGGTGGATTGGCGGTATTGCATTGATGGGAATAGATTTCCCTTTAATCATGCAATATGA
AAAAGTGGGCGAGGCTCTACTGTTACTTGCCTTTATTTTTTATCTTTATAAGCGCCCCATGTTCGTTGATCGACCATTTT
TAAAGGCAGTGTTTTGCTTATTATGTACAACAAGTCTTTTTATGGTTGGTTACCACTATGCTGAAAAAGCATTGATTGAA
CGATTAGAACAAAGAGAAACAGATACCCGAAATCTCGACATTATTGTTTATATAAACCGTTTAAGTGAAGAAAAAGATAA
TAAGGTTCAACAAACTGCACAAGTTCTAAATCTTTCTAAAGAACCGGTGAATTGGTTGCTATATTTAAAAAGTAATAATC
AAAATTTATTAAAGAATAATCAGAATCTTGAATTAGGTCACTATTATCGAATATCTGGAAAAACAAGACCTGCGCATAGT
TATGCCACCCCAGGATCTTTTGATCAGGAAAAGTGGTTTATTCAGCGAAATATTATGTCTGGTTTTAATGTGAGATATAT
TGAGCCTTTAAGTCTCGATGAAATCTATCGATTGGGCTATCAGCAACATTTAAAAGAACAACAGTCTTTTTCCAGTAGTT
TTCGTTTAAATATAGAAAAACTTCGCTTAACTTTTAGGCAAATATTAAACAGCTCATCTCTACAGCAAAAGGGTTTAATT
TTAGCTTTGCTGACAGGTGATGAAAGCCTTTTATCAGATGAAACTCAACTACAGTTCAAACAATTAGGAATTAGTCATTT
ATTGGCGATCTCAGGCCCACATGTGTTCATTTTTGCCATTATGTTATCTTGGGCATGTCATCAATTTATCAGTCGTTATT
ATCCTAAAATTTACTTATGGAAACCGAAACAGATTTTGATGGCTGTACCATGCTGCCTTGGTGTTTTAATTTATACTGCA
TTTGTAGGTTTCGAAATTCCTGCACTACGAACATTATTATCAACCCTTATATTTATTGGATTTCTATTATTAAAACAACC
TATTAAACCTTTTACATTACTCGTATATAGCGCAAGTCTACTGTTGCTAATGGACCCGTTTAGTGTGCTTTCTGCAGGTT
TTTGGCTGTCTTATGGGGCATGTTTTATTTTAATAAGAATTTACCAAACTATAGCGCAGCTACCTGAGCAACATTTTTTA
AGTCTGAGTTCAAAAATGATTTTTATGAGTAAGGTATTAATTGAATCTCAAGGCAAAATATTTATTGCATTGAGTCCCTT
AACCTTACTTTTCTTTCAACGAATTTCTTGGGTTGCTCCATTAACCAATATTATTGCCGTGCCTATTGTTGGTAGTGTTA
TTGTCCCTTTAAACATCATAGCTGCTTGCGCATGGTTTGTAGTAAAACCATTTGGAAATATGCTTTTTCATTTCAATGAT
ATGTTGCTCAGCATATTGCTGAGTTGTTTGGGCTTATTAGAAAAACTCTCTTTACCATTACAAGGTATAAGCTTGACGCC
ACTGTCTTTATTAGCGATTAGTTGTGCCATAATTATTTTATTTTTACCTAAAGGAATTCTGCCCAAAACTTGGGGAATAT
TATGTTGTTTACCCCTAGTTATGATGAACAAAACGAGTCAGCAAATTCAGCTTAATATTTTAGATGTTGGCCAGGGGCAG
GCTATTTTTCTACAACATCCCCAACAAAACTGGTTAATTGATACAGGCGGTTCTTACGATGAAAAAATATTTAGTATTGG
ACAAAATGTTGTAGTGCCTTTTCTACGTCAGCAAGGCGTAAGACAATTAGATCATGTTGTGCTATCCCATCTTGATCAAG
ACCATAGTGGCGCGTTTCCTATTATTCAACAAGAGATTCCTGTAAAGCAGCTTATTTCGAATGAACAATTACCAAATGAT
TTAAAGCAACCATTCCAATATTGCCATCAAGGGCAACAATGGCATTATTCTGAGTTAGATATTCAAATTTTATGGCCTAA
AGAAAAAGATCTGGCTTTTGTTGCTTCTAATCAGAATCAATATTCTTGTGTTGTATATCTTCAATTTAAAAAAGTTGGTG
GTTACCAAAATTTTCTTATTATGGGCGATGCTGGATGGGAAGCTGAATACGAGTTATTAAAAGATTATCCTAACTTGAAG
ATAGATGTGTTAGTGCTAGGGCATCATGGAAGTAAGCATAGTTCGGCTTATGATTTCTTGGCGACCTTAAAACCTAAACT
GGCCATTGCATCGGCAGGGTTTGATAACCGTTATGGCCATCCTAGCCAACAAGTTATAGCACGTTTAAAAGCTCTGCATA
TTCCGCTAAAAAGTACTTTGGAACAAGGGACCTTAAGTTTTGTGCTGGAAAACCACAAATTAGTTTTACATGACCGACGT
TTGGATCGGCTCTGGCTGAGTAGAGGTTTTTAA

Domains

Predicted by InterproScan.

• Competence protein (Competence) (243-521)

• Domain of unknown function (DUF4131) (DUF4131) (33-188)

• Metallo-beta-lactamase superfamily (Lactamase_B) (555-638)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comA/comEC	Acinetobacter baumannii D1279779	97.407	100	0.974
comA/comEC	Acinetobacter baylyi ADP1	49.815	100	0.499

References

• [1]: Nina Vesel et al. (2021) Pilus Production in Acinetobacter baumannii Is Growth Phase Dependent and Essential for Natural Transformation. Journal of Bacteriology 203(8):e00034-21. [PMID: 33495250]
• [2]: Casin Le et al. (2021) Involvement of the Histone-Like Nucleoid Structuring Protein (H-NS) in Acinetobacter baumannii's Natural Transformation. Pathogens (Basel, Switzerland) 10(9):1083. [PMID: 34578115]