Detailed information    

experimental Experimentally validated

Overview

Name   pilA   Type   Machinery gene
Locus tag   H0N27_RS01510 Genome accession   NZ_CP059039
Coordinates   330035..330484 (+) Length   149 a.a.
NCBI ID   WP_000993725.1    Uniprot ID   A0A9P2QEL2
Organism   Acinetobacter baumannii strain A118     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

Mutant studies with A. baumannii A118 confirmed that pilA, pilQ (secretin, comQ homologue), pilT (retraction ATPase), comEA (DNA-binding protein), comF (pilotin) and dprA (DNA protecting protein) are required for natural transformation


Genomic Context

Location: 325035..335484
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H0N27_RS01490 (H0N27_01490) - 326425..327312 (+) 888 WP_001017319.1 metal-dependent hydrolase -
  H0N27_RS01495 (H0N27_01495) - 327443..328033 (+) 591 WP_000846931.1 LemA family protein -
  H0N27_RS01500 (H0N27_01500) - 328055..329137 (+) 1083 WP_168726694.1 TPM domain-containing protein -
  H0N27_RS01505 (H0N27_01505) - 329131..329691 (+) 561 WP_000258949.1 TPM domain-containing protein -
  H0N27_RS01510 (H0N27_01510) pilA 330035..330484 (+) 450 WP_000993725.1 pilin Machinery gene
  H0N27_RS01515 (H0N27_01515) - 330667..332295 (+) 1629 WP_168726695.1 PglL family O-oligosaccharyltransferase -
  H0N27_RS01520 (H0N27_01520) bfr 332335..332799 (-) 465 WP_000678123.1 bacterioferritin -
  H0N27_RS01525 (H0N27_01525) - 333044..333238 (-) 195 WP_000289091.1 bacterioferritin-associated ferredoxin -
  H0N27_RS01530 (H0N27_01530) - 333448..333831 (-) 384 WP_000090000.1 RidA family protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  hns pilA negative effect
  hns pilQ negative effect
  hns pilT negative effect
  hns comA/comEC negative effect
  hns comF negative effect
  pilR pilA positive effect
  hns dprA negative effect
  hns comEA negative effect
  pilS pilR positive effect

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15475.66 Da        Isoelectric Point: 4.5701

>NTDB_id=1038 H0N27_RS01510 WP_000993725.1 330035..330484(+) (pilA) [Acinetobacter baumannii strain A118]
MNAQKGFTLIELMIVVAIIGILAAIAIPQYQNYIAKSQVNSGFSEISSIKTGYENAVNEGAVPTKLSDVGFTAATSSVCA
TYSIDAFTETDGAGKITCTLKGNPKIATKIVELTRSTDGAWTCVSDVPTEFLPKGCTAGTPTEGAITSL

Nucleotide


Download         Length: 450 bp        

>NTDB_id=1038 H0N27_RS01510 WP_000993725.1 330035..330484(+) (pilA) [Acinetobacter baumannii strain A118]
ATGAATGCACAAAAAGGTTTTACATTAATCGAACTCATGATCGTGGTTGCTATTATCGGTATTTTGGCAGCAATTGCGAT
TCCGCAATATCAAAACTATATCGCTAAATCACAAGTGAACAGTGGATTTTCTGAAATCAGTTCTATTAAAACTGGATATG
AAAATGCTGTAAATGAAGGAGCAGTTCCTACCAAGTTATCTGATGTTGGTTTTACTGCAGCTACCTCTAGTGTTTGTGCT
ACATATAGTATTGACGCATTTACTGAAACAGATGGAGCAGGTAAGATTACTTGTACTCTTAAAGGTAATCCAAAAATTGC
TACTAAGATAGTTGAATTAACTCGTTCTACAGATGGTGCTTGGACTTGTGTTTCTGATGTGCCGACAGAGTTTTTACCAA
AAGGTTGTACCGCAGGTACACCAACAGAAGGTGCTATCACTAGTTTGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter nosocomialis M2

45.89

100

0.472
  pilA Pseudomonas aeruginosa PAK

45.333

100

0.456
  pilA/pilAI Pseudomonas stutzeri DSM 10701

45.985

98.561

0.453
  pilA Vibrio cholerae C6706

42.667

100

0.43
  pilA Vibrio cholerae strain A1552

42.667

100

0.43
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.667

100

0.43
  pilA2 Legionella pneumophila str. Paris

39.726

100

0.426
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.796

97.163

0.426
  pilA2 Legionella pneumophila strain ERS1305867

39.041

100

0.419
  comP Acinetobacter baylyi ADP1

40

98.639

0.395
  comF Acinetobacter baylyi ADP1

37.063

99.306

0.368

Multiple sequence alignment    



References

[1] Nina Vesel et al. (2021) Pilus Production in Acinetobacter baumannii Is Growth Phase Dependent and Essential for Natural Transformation. Journal of Bacteriology 203(8):e00034-21. [PMID: 33495250]
[2] Casin Le et al. (2021) Involvement of the Histone-Like Nucleoid Structuring Protein (H-NS) in Acinetobacter baumannii's Natural Transformation. Pathogens (Basel, Switzerland) 10(9):1083. [PMID: 34578115]