Detailed information NTG0001192

Experimentally validated

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: PAKAF_RS23870
• Genome accession: NZ_LR657304
• Coordinates: 5177838..5178290 (-)
• Length: 150 a.a.
• NCBI ID: WP_024947914.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa PAK
• Function: assembly of type IV pilus DNA binding and uptake

Function

P. aeruginosa in biofilms is competent for natural transformation of both genomic and plasmid DNA. All T4P mutants were capable of some natural transformation of pUCPSK, however a significant reduction in transformation frequency compared to wild-type PAK was observed.

Genomic Context

Location: 5172838..5183290

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PAKAF_RS23850 (PAKAF_04793)	ampD	5173544..5174110 (-)	567	WP_016253886.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
PAKAF_RS23855 (PAKAF_04794)	-	5174261..5176516 (-)	2256	WP_016253887.1	DUF1631 domain-containing protein	-
PAKAF_RS23860 (PAKAF_04795)	nadC	5176802..5177650 (+)	849	WP_003116246.1	carboxylating nicotinate-nucleotide diphosphorylase	-
PAKAF_RS23870 (PAKAF_05939)	pilA	5177838..5178290 (-)	453	WP_024947914.1	pilin	Machinery gene
PAKAF_RS23875 (PAKAF_04797)	pilB	5178519..5180219 (+)	1701	WP_009875880.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PAKAF_RS23880 (PAKAF_04798)	pilC	5180223..5181443 (+)	1221	WP_016253888.1	type 4a pilus biogenesis protein PilC	Machinery gene
PAKAF_RS23885 (PAKAF_04799)	pilD	5181447..5182319 (+)	873	WP_003125204.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
PAKAF_RS23890 (PAKAF_04800)	coaE	5182316..5182927 (+)	612	WP_003112838.1	dephospho-CoA kinase	-
PAKAF_RS23895 (PAKAF_04801)	yacG	5182924..5183124 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

• Length: 150 a.a.
• Molecular weight: 15622.02 Da
• Isoelectric Point: 8.4827

>NTDB_id=1192 PAKAF_RS23870 WP_024947914.1 5177838..5178290(-) (pilA) [Pseudomonas aeruginosa PAK]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALASVNPLKTTVEEALSRGWSVKSGTGTEDATKKEVPLG
VAADANKLGTIALKPDPADGTADITLTFTMGGAGPKNKGKIITLTRTAADGLWKCTSDQDEQFIPKGCSK

Nucleotide

• Length: 453 bp

>NTDB_id=1192 PAKAF_RS23870 WP_024947914.1 5177838..5178290(-) (pilA) [Pseudomonas aeruginosa PAK]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCTTGGCTGCAATTGCCAT
TCCTCAGTATCAGAATTATGTAGCTCGTTCGGAAGGCGCATCTGCTCTTGCTTCGGTCAATCCGTTGAAGACTACCGTTG
AAGAGGCGCTTTCTCGTGGTTGGAGCGTGAAGAGCGGTACAGGTACAGAGGACGCTACTAAGAAAGAGGTTCCTCTGGGG
GTGGCGGCAGATGCTAACAAACTGGGTACTATCGCACTCAAACCCGATCCTGCTGATGGTACTGCAGATATCACTTTGAC
TTTCACTATGGGCGGTGCAGGACCGAAGAATAAAGGGAAAATTATTACCCTGACTCGTACTGCAGCTGATGGTCTCTGGA
AGTGCACCAGTGATCAGGATGAGCAGTTTATTCCGAAAGGTTGCTCTAAGTAA

Domains

Predicted by InterproScan.

• Pilin (bacterial filament) (Pilin) (35-148)

• Prokaryotic N-terminal methylation motif (N_methyl) (1-26)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA/pilAI	Pseudomonas stutzeri DSM 10701	47.682	100	0.518
pilA/pilAII	Pseudomonas stutzeri DSM 10701	46.575	100	0.482
pilA	Vibrio parahaemolyticus RIMD 2210633	48.507	96.403	0.468
pilA	Acinetobacter baumannii strain A118	45.333	100	0.456
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	42.675	100	0.447
pilA	Vibrio cholerae C6706	42.675	100	0.447
pilA	Vibrio cholerae strain A1552	42.675	100	0.447
pilA2	Legionella pneumophila strain ERS1305867	39.865	100	0.434
pilA2	Legionella pneumophila str. Paris	39.189	100	0.426
comP	Acinetobacter baylyi ADP1	40.789	100	0.422
pilA/pilA1	Eikenella corrodens VA1	39.456	98	0.387
pilA	Acinetobacter nosocomialis M2	45.763	83.099	0.38

References

• [1]: Laura M Nolan et al. (2020) Pseudomonas aeruginosa is capable of natural transformation in biofilms. Microbiology (Reading, England) 166(10):995-1003. [PMID: 32749953]