Detailed information    

experimental Experimentally validated

Overview

Name   pilA/pilA1   Type   Machinery gene
Locus tag   - Genome accession   AF079304
Coordinates   484..945 (+) Length   153 a.a.
NCBI ID   AAC28468.1    Uniprot ID   -
Organism   Eikenella corrodens VA1     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

Strain with pilA inactivated was not competent for transformation, nor did it exhibit twitching motility.


Genomic Context

Location: 1..5945
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Locus_0 pilA/pilA1 484..945 (+) 462 AAC28468.1 type IV pilin Machinery gene
  Locus_1 pilA/pilA1 1013..1462 (+) 450 AAC28469.1 type IV pilin Machinery gene
  Locus_2 pilB 1550..2152 (+) 603 AAC28470.1 putative fimbrial protein -
  Locus_3 hagA 2463..3326 (+) 864 AAC28471.1 putative hemagglutinin protein -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 15661.97 Da        Isoelectric Point: 7.7847

>NTDB_id=1191 AAC28468.1 484..945(+) (pilA/pilA1) [Eikenella corrodens VA1]
MLKKVQKGFTLIELMIVIAIIGILAAIALPAYQDYTARAQATEGFKATAGLQTDLGAWRADRGSFPNAAALVAGNVIFDS
ANGLDGKYFAPGGVTVTPDTGAINIAFDAGANAGKTMTITPTVIAASGQISTWTCAPGAANGIETRRLPTSCQ*

Nucleotide


Download         Length: 462 bp        

>NTDB_id=1191 AAC28468.1 484..945(+) (pilA/pilA1) [Eikenella corrodens VA1]
ATGTTGAAAAAAGTACAAAAAGGTTTCACCCTGATTGAGTTGATGATTGTTATCGCCATTATCGGTATTTTGGCCGCTAT
CGCCCTGCCGGCTTACCAAGACTACACTGCCCGTGCTCAGGCTACTGAAGGTTTCAAAGCTACTGCTGGTCTGCAAACTG
ATTTGGGTGCTTGGCGTGCTGATCGTGGTTCATTCCCGAATGCTGCCGCTTTGGTTGCCGGTAACGTAATTTTTGATAGC
GCTAATGGTTTGGATGGTAAATACTTTGCTCCTGGTGGCGTGACTGTAACTCCTGATACTGGCGCTATTAACATTGCTTT
TGATGCGGGTGCAAACGCTGGTAAAACTATGACTATTACTCCGACTGTGATTGCCGCTTCCGGTCAGATCTCTACTTGGA
CTTGCGCTCCTGGTGCTGCTAACGGTATCGAAACTCGCCGTCTGCCAACTAGCTGCCAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-153)

Prokaryotic N-terminal methylation motif (N_methyl)

(4-29)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila strain ERS1305867

39.873

100

0.463
  pilA2 Legionella pneumophila str. Paris

39.873

100

0.463
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.49

100

0.446
  pilE Neisseria gonorrhoeae strain FA1090

38.415

100

0.409
  comP Acinetobacter baylyi ADP1

38.462

100

0.408
  pilA Vibrio campbellii strain DS40M4

38.125

100

0.396
  pilE Neisseria gonorrhoeae MS11

35.465

100

0.396
  pilA Ralstonia pseudosolanacearum GMI1000

34.857

100

0.396
  pilA Pseudomonas aeruginosa PAK

39.456

98

0.387

Multiple sequence alignment    



References

[1] M T Villar et al. (2001) Role of the Eikenella corrodens pilA locus in pilus function and phase variation. Journal of Bacteriology 183(1):55-62. [PMID: 11114900]