Detailed information    

experimental Experimentally validated

Overview

Name   pilE   Type   Machinery gene
Locus tag   NGFG_RS11465 Genome accession   NC_022240
Coordinates   2109185..2109685 (-) Length   166 a.a.
NCBI ID   WP_017147169.1    Uniprot ID   -
Organism   Neisseria gonorrhoeae MS11     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

ComP functions in a dose-dependent manner in conjunction with PilE to promote DNA uptake.


Genomic Context

Location: 2104185..2114685
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGFG_RS11450 (NGFG_01824) msrAB 2104633..2106201 (-) 1569 WP_003696288.1 bifunctional peptide-methionine (S)-S-oxide reductase MsrA/peptide-methionine (R)-S-oxide reductase MsrB -
  NGFG_RS11455 (NGFG_01822) pilA 2106347..2107600 (+) 1254 WP_003696286.1 signal recognition particle-docking protein FtsY Machinery gene
  NGFG_RS15335 - 2107636..2107854 (-) 219 WP_157778910.1 hypothetical protein -
  NGFG_RS11460 (NGFG_02483) - 2107868..2108541 (-) 674 Protein_2146 opacity family porin -
  NGFG_RS11465 (NGFG_01821) pilE 2109185..2109685 (-) 501 WP_017147169.1 pilin Machinery gene
  NGFG_RS11470 - 2110056..2110199 (-) 144 WP_003691943.1 hypothetical protein -
  NGFG_RS11475 (NGFG_01819) - 2110458..2110841 (-) 384 Protein_2149 pilin -
  NGFG_RS11480 (NGFG_01818) pilE 2110959..2111390 (-) 432 WP_003696281.1 pilin Machinery gene
  NGFG_RS11485 (NGFG_01817) lpxC 2111496..2112419 (-) 924 WP_003696279.1 UDP-3-O-acyl-N-acetylglucosamine deacetylase -
  NGFG_RS11490 (NGFG_02216) pilE 2112950..2113369 (-) 420 WP_017147167.1 pilin Machinery gene
  NGFG_RS11495 (NGFG_02216) - 2113465..2113884 (-) 420 Protein_2153 pilin -

Sequence

Protein


Download         Length: 166 a.a.        Molecular weight: 17939.45 Da        Isoelectric Point: 7.8401

>NTDB_id=1119 NGFG_RS11465 WP_017147169.1 2109185..2109685(-) (pilE) [Neisseria gonorrhoeae MS11]
MNTLQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQVSEAILLAEGQKSAVTEYYLNHGKWPENNTSAGVASPPTDI
KGKYVKEVEVKNGVVTATMLSSGVNNEIKGKKLSLWGRRENGSVKWFCGQPVTRADDDTVADAKDGKEIDTKHLPSTCRD
KASDAK

Nucleotide


Download         Length: 501 bp        

>NTDB_id=1119 NGFG_RS11465 WP_017147169.1 2109185..2109685(-) (pilE) [Neisseria gonorrhoeae MS11]
ATGAATACCCTTCAAAAAGGCTTTACCCTTATCGAGCTGATGATTGTGATCGCTATCGTCGGCATTTTGGCGGCAGTCGC
CCTTCCCGCCTACCAAGACTACACCGCCCGCGCGCAAGTTTCCGAAGCCATCCTTTTGGCCGAAGGTCAAAAATCAGCCG
TCACCGAGTATTACCTGAATCACGGCAAATGGCCGGAAAACAACACTTCTGCCGGCGTGGCATCCCCCCCCACCGACATC
AAAGGCAAATATGTTAAAGAGGTTGAAGTTAAAAACGGCGTCGTTACCGCCACAATGCTTTCAAGCGGCGTAAACAATGA
AATCAAAGGCAAAAAACTCTCCCTGTGGGGCAGGCGTGAAAACGGTTCGGTAAAATGGTTCTGCGGACAGCCGGTTACGC
GCGCCGACGACGACACCGTTGCCGACGCCAAAGACGGCAAAGAAATCGACACCAAGCACCTGCCGTCAACCTGCCGCGAT
AAGGCATCTGATGCCAAATGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-159)

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

81.212

100

0.817
  comP Acinetobacter baylyi ADP1

39.286

100

0.449
  pilA2 Legionella pneumophila strain ERS1305867

38.217

100

0.441
  pilA2 Legionella pneumophila str. Paris

37.179

100

0.426
  pilA/pilA1 Eikenella corrodens VA1

35.465

100

0.396
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

34.031

100

0.392

Multiple sequence alignment    



References

[1] Finn Erik Aas et al. (2002) Competence for natural transformation in Neisseria gonorrhoeae: components of DNA binding and uptake linked to type IV pilus expression. Molecular Microbiology 46(3):749-60. [PMID: 12410832]