Detailed information    

experimental Experimentally validated

Overview

Name   pilA   Type   Machinery gene
Locus tag   NGFG_RS11455 Genome accession   NC_022240
Coordinates   2106347..2107600 (+) Length   417 a.a.
NCBI ID   WP_003696286.1    Uniprot ID   A0AB74ETB0
Organism   Neisseria gonorrhoeae MS11     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

PilA, the Tfp subunit protein of P. aeruginosa, functions in gonococcal transformation.


Genomic Context

Location: 2101347..2112600
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGFG_RS11435 (NGFG_01827) - 2102094..2103095 (+) 1002 WP_003691948.1 thiamine ABC transporter substrate-binding protein -
  NGFG_RS11440 (NGFG_01826) - 2103189..2104037 (+) 849 WP_003687000.1 mechanosensitive ion channel family protein -
  NGFG_RS11445 (NGFG_01825) - 2104064..2104549 (+) 486 WP_003687001.1 CinA family protein -
  NGFG_RS11450 (NGFG_01824) msrAB 2104633..2106201 (-) 1569 WP_003696288.1 bifunctional peptide-methionine (S)-S-oxide reductase MsrA/peptide-methionine (R)-S-oxide reductase MsrB -
  NGFG_RS11455 (NGFG_01822) pilA 2106347..2107600 (+) 1254 WP_003696286.1 signal recognition particle-docking protein FtsY Machinery gene
  NGFG_RS15335 - 2107636..2107854 (-) 219 WP_157778910.1 hypothetical protein -
  NGFG_RS11460 (NGFG_02483) - 2107868..2108541 (-) 674 Protein_2146 opacity family porin -
  NGFG_RS11465 (NGFG_01821) pilE 2109185..2109685 (-) 501 WP_017147169.1 pilin Machinery gene
  NGFG_RS11470 - 2110056..2110199 (-) 144 WP_003691943.1 hypothetical protein -
  NGFG_RS11475 (NGFG_01819) - 2110458..2110841 (-) 384 Protein_2149 pilin -
  NGFG_RS11480 (NGFG_01818) pilE 2110959..2111390 (-) 432 WP_003696281.1 pilin Machinery gene
  NGFG_RS11485 (NGFG_01817) lpxC 2111496..2112419 (-) 924 WP_003696279.1 UDP-3-O-acyl-N-acetylglucosamine deacetylase -

Sequence

Protein


Download         Length: 417 a.a.        Molecular weight: 44331.70 Da        Isoelectric Point: 4.8647

>NTDB_id=1118 NGFG_RS11455 WP_003696286.1 2106347..2107600(+) (pilA) [Neisseria gonorrhoeae MS11]
MFSFFRRKKKQETPALEEAQVQETAAKVESEVAQIVGNIKEDVESLAESVKGRAESAVETVSGAVEQVKETVAEMPSEAG
EAAERVESAKEAVAETVGEAVGQVQEAVATTEEHKLGWAARLKQGLAKSRDKMAKSLAGVFGGGQIGEDLYEELETVLIT
GDMGMEATEYLMKDVRGRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPETKEPFVIMLAGINGAGKTTSIGKLAKY
FQAQGKSVLLAAGDTFRAAAREQLQAWGGRNNVTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEI
KKVKRVLQKAIPGAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRPVPVRYIGVGEGI
DDLRPFDARAFVDALLD

Nucleotide


Download         Length: 1254 bp        

>NTDB_id=1118 NGFG_RS11455 WP_003696286.1 2106347..2107600(+) (pilA) [Neisseria gonorrhoeae MS11]
ATGTTCAGCTTCTTCCGTCGCAAGAAAAAACAGGAAACGCCGGCTCTCGAGGAGGCCCAAGTTCAGGAAACCGCAGCAAA
AGTAGAATCTGAAGTTGCTCAAATAGTTGGAAATATTAAAGAAGATGTCGAATCTTTAGCAGAAAGCGTCAAAGGGCGGG
CCGAATCTGCCGTTGAAACCGTCAGCGGTGCGGTTGAACAGGTAAAGGAAACCGTTGCCGAGATGCCGTCTGAAGCAGGG
GAAGCGGCGGAACGCGTTGAATCCGCAAAAGAAGCTGTTGCCGAAACCGTCGGCGAGGCTGTCGGGCAAGTTCAAGAAGC
CGTTGCGACAACTGAAGAACACAAGCTCGGTTGGGCTGCGCGTTTGAAACAAGGCTTGGCCAAATCACGCGACAAAATGG
CGAAATCGCTGGCCGGCGTGTTCGGCGGCGGACAAATCGGCGAGGATTTGTACGAAGAGCTGGAAACCGTGCTGATTACC
GGCGATATGGGCATGGAGGCCACCGAATACCTGATGAAAGACGTGCGCGGCCGCGTCAGCCTCAAAGGGCTGAAAGACGG
CAACGAATTGCGCGGCGCGTTGAAAGAAGCCTTGTACGACCTGATTAAGCCGTTGGAAAAACCGCTGGTCTTGCCCGAAA
CTAAAGAGCCTTTCGTGATTATGCTTGCCGGTATCAACGGCGCGGGCAAAACCACGTCTATCGGCAAACTCGCCAAATAT
TTCCAAGCGCAGGGCAAATCCGTATTGCTGGCGGCGGGCGATACCTTCCGCGCCGCCGCCCGTGAGCAGCTTCAGGCTTG
GGGCGGGCGCAACAATGTAACCGTCATTTCACAAACCACGGGCGATTCCGCCGCCGTGTGCTTCGATGCCGTCCAAGCCG
CCAAAGCGCGCGGGATCGACATCGTGCTTGCCGACACCGCCGGCCGCCTGCCCACGCAGCTTCATTTGATGGAAGAAATC
AAAAAAGTGAAGCGCGTGCTGCAAAAAGCCATTCCCGGCGCGCCGCACGAAATTATCGTCGTACTCGATGCCAATATCGG
GCAAAACGCCGTCAACCAAGTCAAAGCCTTTGACGACGCATTGGGGCTGACGGGGCTTATCGTTACCAAACTCGACGGCA
CGGCAAAAGGCGGCATCCTCGCCGCGCTTGCTTCCGACCGCCCCGTCCCCGTCCGCTACATCGGCGTGGGCGAAGGCATA
GACGACCTGCGCCCGTTTGACGCGCGCGCGTTTGTGGACGCACTGCTGGATTGA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(217-416)

SRP54-type protein, helical bundle domain (SRP54_N)

(132-198)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Finn Erik Aas et al. (2002) Competence for natural transformation in Neisseria gonorrhoeae: components of DNA binding and uptake linked to type IV pilus expression. Molecular Microbiology 46(3):749-60. [PMID: 12410832]