Detailed information NTG0001188

Experimentally validated

Overview

• Name: pilE
• Type: Machinery gene
• Locus tag: NELON_RS01755
• Genome accession: NZ_CP007726
• Coordinates: 346430..346996 (-)
• Length: 188 a.a.
• NCBI ID: WP_003772458.1
• Uniprot ID: D4DRI6
• Organism: Neisseria elongata subsp. glycolytica ATCC 29315
• Function: assembly of type IV pilus DNA binding and uptake

Function

The N. elongata pilE null mutant does not produce Tfp and is significantly less transformable.

Genomic Context

Location: 341430..351996

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NELON_RS01730 (NELON_01820)	mlaE	341884..342660 (-)	777	WP_003772451.1	lipid asymmetry maintenance ABC transporter permease subunit MlaE	-
NELON_RS01735 (NELON_01825)	-	342723..343523 (-)	801	WP_003772452.1	ABC transporter ATP-binding protein	-
NELON_RS01740 (NELON_01830)	apbC	343812..344891 (+)	1080	WP_041961261.1	iron-sulfur cluster carrier protein ApbC	-
NELON_RS01745 (NELON_01835)	-	345008..345799 (-)	792	WP_003772456.1	hypothetical protein	-
NELON_RS01750 (NELON_01840)	-	345829..346359 (-)	531	WP_040665936.1	pilin	-
NELON_RS01755 (NELON_01845)	pilE	346430..346996 (-)	567	WP_003772458.1	pilin	Machinery gene
NELON_RS01760 (NELON_01850)	-	347582..348679 (-)	1098	WP_003772463.1	NnrS family protein	-
NELON_RS01765 (NELON_01855)	-	348975..349589 (-)	615	WP_003772465.1	CadD family cadmium resistance transporter	-
NELON_RS01770 (NELON_01860)	-	349900..350592 (-)	693	WP_174428826.1	ATP-binding cassette domain-containing protein	-
NELON_RS01775 (NELON_01865)	-	350592..351389 (-)	798	WP_041961262.1	ABC transporter permease	-

Sequence

Protein

• Length: 188 a.a.
• Molecular weight: 18864.68 Da
• Isoelectric Point: 9.4112

>NTDB_id=1188 NELON_RS01755 WP_003772458.1 346430..346996(-) (pilE) [Neisseria elongata subsp. glycolytica ATCC 29315]
MKAMQKGFTLIELMIVIAIIGILAAIALPAYQDYTVRSKVSEALIAASSPKSALSEAFQTDGITGMTAAAKAFNKTAAAG
GGAGGAAAAGTQHASKYVKSVKVTEASPWDITVAISATEANGIPTGLDGNTIRLFPLVNKAVPAATSTGAIDWACASAAK
ETADKRFGTGSGVAVGTLPAKYAPSECK

Nucleotide

• Length: 567 bp

>NTDB_id=1188 NELON_RS01755 WP_003772458.1 346430..346996(-) (pilE) [Neisseria elongata subsp. glycolytica ATCC 29315]
ATGAAAGCAATGCAAAAAGGTTTCACCCTGATCGAGTTGATGATCGTAATCGCCATTATCGGTATTTTGGCTGCTATCGC
CCTGCCGGCTTACCAAGACTACACTGTACGTTCTAAAGTTTCCGAAGCTTTGATCGCTGCTTCTTCTCCGAAGTCTGCTC
TGTCTGAAGCTTTCCAAACTGACGGCATTACCGGTATGACTGCAGCTGCCAAAGCATTTAACAAAACCGCTGCTGCTGGT
GGTGGTGCTGGTGGTGCTGCTGCTGCTGGTACTCAACATGCATCCAAATATGTTAAAAGCGTGAAAGTTACTGAAGCTAG
CCCATGGGATATTACCGTAGCCATTTCCGCTACAGAGGCAAACGGTATTCCGACTGGTTTAGACGGAAATACAATCAGAC
TGTTCCCGTTGGTTAATAAAGCGGTTCCGGCAGCAACTTCTACCGGCGCGATTGACTGGGCTTGTGCTTCTGCAGCAAAA
GAAACTGCGGATAAACGTTTCGGTACTGGTTCTGGCGTTGCTGTTGGTACCTTGCCTGCCAAATACGCTCCTTCTGAGTG
CAAATAA

Domains

Predicted by InterproScan.

• Pilin (bacterial filament) (Pilin) (36-188)

• Prokaryotic N-terminal methylation motif (N_methyl) (4-28)

3D structure

• AlphaFold DB: D4DRI6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA2	Legionella pneumophila strain ERS1305867	37.566	100	0.522
pilA2	Legionella pneumophila str. Paris	37.566	100	0.522
comP	Acinetobacter baylyi ADP1	38.587	100	0.483
pilA/pilAI	Pseudomonas stutzeri DSM 10701	34.359	100	0.482
pilA	Ralstonia pseudosolanacearum GMI1000	36.082	100	0.417
pilE	Neisseria gonorrhoeae strain FA1090	34.211	100	0.396
pilE	Neisseria gonorrhoeae MS11	34.031	100	0.392

References

• [1]: Dustin L Higashi et al. (2011) N. elongata produces type IV pili that mediate interspecies gene transfer with N. gonorrhoeae. PloS One 6(6):e21373. [PMID: 21731720]