Detailed information    

experimental Experimentally validated

Overview

Name   pilE   Type   Machinery gene
Locus tag   OK783_RS10670 Genome accession   NZ_CP115654
Coordinates   2038466..2038960 (-) Length   164 a.a.
NCBI ID   WP_157147416.1    Uniprot ID   -
Organism   Neisseria gonorrhoeae strain FA1090     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

ΔpilE mutant was non-competent for transformation.


Genomic Context

Location: 2033466..2043960
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OK783_RS10645 (OK783_10645) - 2033494..2033976 (+) 483 WP_003694396.1 CinA family protein -
  OK783_RS10650 (OK783_10650) msrAB 2034060..2035628 (-) 1569 WP_010951394.1 bifunctional peptide-methionine (S)-S-oxide reductase MsrA/peptide-methionine (R)-S-oxide reductase MsrB -
  OK783_RS10655 (OK783_10655) pilA 2035774..2037039 (+) 1266 WP_010951395.1 signal recognition particle-docking protein FtsY Machinery gene
  OK783_RS10660 (OK783_10660) - 2037124..2037294 (-) 171 WP_164920398.1 hypothetical protein -
  OK783_RS10665 (OK783_10665) - 2037308..2037991 (-) 684 WP_030003519.1 opacity family porin -
  OK783_RS10670 (OK783_10670) pilE 2038466..2038960 (-) 495 WP_157147416.1 pilin Machinery gene
  OK783_RS10680 (OK783_10680) pilE 2039776..2040198 (-) 423 WP_157147417.1 pilin Machinery gene
  OK783_RS10685 (OK783_10685) lpxC 2040304..2041227 (-) 924 WP_003687007.1 UDP-3-O-acyl-N-acetylglucosamine deacetylase -
  OK783_RS10690 (OK783_10690) - 2041801..2042142 (-) 342 Protein_2044 pilin -
  OK783_RS10695 (OK783_10695) - 2042195..2042545 (-) 351 Protein_2045 pilin -
  OK783_RS10700 (OK783_10700) - 2042585..2042968 (-) 384 Protein_2046 pilin -
  OK783_RS10705 (OK783_10705) - 2043409..2043740 (-) 332 Protein_2047 pilin -

Sequence

Protein


Download         Length: 164 a.a.        Molecular weight: 17604.00 Da        Isoelectric Point: 7.1324

>NTDB_id=1109 OK783_RS10670 WP_157147416.1 2038466..2038960(-) (pilE) [Neisseria gonorrhoeae strain FA1090]
MNTLQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQVSEAILLAEGQKSAVTEYYLNHGIWPKDNTSAGVASASTIK
GKYVQKVEVAKGVVTAQMASTGVNKEIQDKKLSLWAKRQDGSVKWFCGQPVTRTGDNDDTVADANNAIDTKHLPSTCRDE
SSAT

Nucleotide


Download         Length: 495 bp        

>NTDB_id=1109 OK783_RS10670 WP_157147416.1 2038466..2038960(-) (pilE) [Neisseria gonorrhoeae strain FA1090]
ATGAATACCCTTCAAAAAGGCTTTACCCTTATCGAGCTGATGATTGTGATCGCTATCGTCGGCATTTTGGCGGCAGTCGC
CCTTCCCGCCTACCAAGACTACACCGCCCGCGCGCAAGTTTCCGAAGCCATCCTTTTGGCCGAAGGTCAAAAATCAGCCG
TCACCGAGTATTACCTGAATCACGGCATATGGCCGAAAGACAACACTTCTGCCGGCGTGGCATCCGCTTCAACAATCAAA
GGCAAATATGTTCAGAAAGTTGAAGTCGCAAAAGGCGTCGTTACCGCCCAAATGGCTTCAACCGGCGTAAACAAAGAAAT
CCAAGACAAAAAACTCTCCCTGTGGGCCAAGCGTCAAGACGGTTCGGTAAAATGGTTCTGCGGACAGCCGGTTACGCGCA
CCGGCGACAACGACGACACCGTTGCCGACGCCAACAACGCCATCGACACCAAGCACCTGCCGTCAACCTGCCGCGATGAA
TCATCTGCCACCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-158)

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae MS11

81.212

100

0.817
  pilA2 Legionella pneumophila strain ERS1305867

38.462

100

0.441
  pilA2 Legionella pneumophila str. Paris

36.943

100

0.426
  pilA/pilA1 Eikenella corrodens VA1

38.415

100

0.409
  comP Acinetobacter baylyi ADP1

36.42

100

0.401
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

34.211

100

0.396

Multiple sequence alignment    



References

[1] Kyle P Obergfell et al. (2016) The Pilin N-terminal Domain Maintains Neisseria gonorrhoeae Transformation Competence during Pilus Phase Variation. PLoS Genetics 12(5):e1006069. [PMID: 27213957]