Detailed information NTG0001258

Experimentally validated

Overview

• Name: pilA2
• Type: Machinery gene
• Locus tag: GCO85_RS09745
• Genome accession: NZ_CP048618
• Coordinates: 2158410..2158820 (-)
• Length: 136 a.a.
• NCBI ID: WP_014844212.1
• Uniprot ID: A0A378K550
• Organism: Legionella pneumophila strain ERS1305867
• Function: assembly of type IV pilus DNA binding and uptake

Function

Both the Tn-seq data and reconstructed mutants show that PilA2 is required for natural transformation, while PilA1 is dispensable.

Genomic Context

Location: 2153410..2163820

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GCO85_RS09730 (GCO85_09740)	letS	2153730..2156462 (+)	2733	WP_080450539.1	ATP-binding protein	Regulator
GCO85_RS09735 (GCO85_09745)	-	2156559..2157803 (+)	1245	WP_011214166.1	6-phosphofructokinase	-
GCO85_RS09740 (GCO85_09750)	pilA2	2157800..2158213 (-)	414	WP_013101573.1	pilin	Machinery gene
GCO85_RS09745 (GCO85_09755)	pilA2	2158410..2158820 (-)	411	WP_014844212.1	pilin	Machinery gene
GCO85_RS09750 (GCO85_09760)	-	2159123..2159440 (+)	318	WP_010947633.1	BolA/IbaG family iron-sulfur metabolism protein	-
GCO85_RS09755 (GCO85_09765)	-	2159515..2160936 (+)	1422	WP_061637041.1	APC family permease	-
GCO85_RS09760 (GCO85_09770)	-	2161031..2162461 (-)	1431	WP_061637044.1	cellulase family glycosylhydrolase	-
GCO85_RS09765 (GCO85_09775)	kdsB	2162709..2163461 (-)	753	WP_061637046.1	3-deoxy-manno-octulosonate cytidylyltransferase	-
GCO85_RS09770 (GCO85_09780)	-	2163458..2163637 (-)	180	WP_010947637.1	Trm112 family protein	-

Sequence

Protein

• Length: 136 a.a.
• Molecular weight: 13925.15 Da
• Isoelectric Point: 8.4715

>NTDB_id=1258 GCO85_RS09745 WP_014844212.1 2158410..2158820(-) (pilA2) [Legionella pneumophila strain ERS1305867]
MRQKGFTLIELMIVVAIIGILAAIAIPAYQDYTIRARVTEGLTLADSAKLAVSETAITNNALPATQAATGYVSPAATPNV
QSIAIGANGVITITYTAAAGGGTIIMTPTLQANGDVTWTCTGGTLLAKYRPASCRP

Nucleotide

• Length: 411 bp

>NTDB_id=1258 GCO85_RS09745 WP_014844212.1 2158410..2158820(-) (pilA2) [Legionella pneumophila strain ERS1305867]
ATGAGACAAAAGGGTTTTACTTTAATTGAGTTGATGATAGTCGTAGCGATTATCGGTATTTTGGCAGCAATCGCTATACC
AGCCTATCAAGATTACACAATACGAGCTCGTGTTACTGAAGGTCTGACCTTGGCAGATTCTGCAAAACTTGCTGTATCTG
AAACGGCTATTACAAACAACGCGTTACCAGCCACTCAGGCTGCAACGGGCTATGTGTCTCCTGCTGCTACGCCCAATGTA
CAATCAATTGCTATTGGTGCTAACGGTGTGATTACCATAACGTATACTGCTGCAGCAGGTGGTGGTACCATTATTATGAC
CCCAACTTTACAAGCAAACGGTGACGTAACCTGGACTTGTACTGGTGGTACATTATTGGCCAAATACCGACCTGCCAGTT
GCAGACCATAA

Domains

Predicted by InterproScan.

• Pilin (bacterial filament) (Pilin) (35-135)

• Prokaryotic N-terminal methylation motif (N_methyl) (2-25)

3D structure

• AlphaFold DB: A0A378K550

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA2	Legionella pneumophila str. Paris	98.529	100	0.985
pilA	Ralstonia pseudosolanacearum GMI1000	49.068	100	0.581
comP	Acinetobacter baylyi ADP1	53.103	100	0.566
pilE	Neisseria elongata subsp. glycolytica ATCC 29315	37.566	100	0.522
pilA/pilA1	Eikenella corrodens VA1	39.873	100	0.463
pilE	Neisseria gonorrhoeae strain FA1090	38.462	100	0.441
pilE	Neisseria gonorrhoeae MS11	38.217	100	0.441
pilA	Haemophilus influenzae 86-028NP	40.816	100	0.441
pilA	Pseudomonas aeruginosa PAK	39.865	100	0.434
pilA/pilAI	Pseudomonas stutzeri DSM 10701	42.963	99.265	0.426
pilA	Vibrio campbellii strain DS40M4	49.242	85.714	0.422
pilA	Acinetobacter baumannii strain A118	39.041	100	0.419
pilA/pilAII	Pseudomonas stutzeri DSM 10701	39.855	100	0.404
pilA	Haemophilus influenzae Rd KW20	43.307	93.382	0.404
pilA	Vibrio cholerae C6706	37.762	100	0.397
pilA	Vibrio cholerae strain A1552	37.762	100	0.397
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	37.762	100	0.397
pilA	Glaesserella parasuis strain SC1401	38.235	100	0.382
pilA	Vibrio parahaemolyticus RIMD 2210633	38.462	95.588	0.368

References

• [1]: Léo Hardy et al. (2021) Transposon Insertion Sequencing in a Clinical Isolate of Legionella pneumophila Identifies Essential Genes and Determinants of Natural Transformation. Journal of Bacteriology 203(3):e00548-20. [PMID: 33168636]