Detailed information NTG0001184

Experimentally validated

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: GTF74_RS02335
• Genome accession: NZ_CP047303
• Coordinates: 484737..485198 (-)
• Length: 153 a.a.
• NCBI ID: WP_000649326.1
• Uniprot ID: A0A0X1KZH2
• Organism: Vibrio cholerae O1 biovar El Tor strain E7946
• Function: assembly of type IV pilus DNA binding and uptake

Function

PilA is the major pilin subunit of Tfp. The non-piliated pilA mutant was deficient in DNA binding.

Genomic Context

Location: 479737..490198

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GTF74_RS02315 (GTF74_02315)	coaE	480242..480850 (-)	609	WP_000011557.1	dephospho-CoA kinase	-
GTF74_RS02320 (GTF74_02320)	pilD	480847..481722 (-)	876	WP_000418747.1	A24 family peptidase	Machinery gene
GTF74_RS02325 (GTF74_02325)	pilC	481780..483006 (-)	1227	WP_000648511.1	type II secretion system F family protein	Machinery gene
GTF74_RS02330 (GTF74_02330)	pilB	483049..484737 (-)	1689	WP_000957200.1	type IV-A pilus assembly ATPase PilB	Machinery gene
GTF74_RS02335 (GTF74_02335)	pilA	484737..485198 (-)	462	WP_000649326.1	pilin	Machinery gene
GTF74_RS02340 (GTF74_02340)	nadC	485443..486333 (-)	891	WP_000665274.1	carboxylating nicotinate-nucleotide diphosphorylase	-
GTF74_RS02345 (GTF74_02345)	ampD	486629..487174 (+)	546	WP_000567318.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
GTF74_RS02350 (GTF74_02350)	fldB	487452..487970 (-)	519	WP_000690112.1	flavodoxin FldB	-
GTF74_RS02355 (GTF74_02355)	xerD	488108..489016 (+)	909	WP_001287472.1	site-specific tyrosine recombinase XerD	-
GTF74_RS02360 (GTF74_02360)	dsbC	489033..489785 (+)	753	WP_000115055.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-

Sequence

Protein

• Length: 153 a.a.
• Molecular weight: 15722.14 Da
• Isoelectric Point: 8.9860

>NTDB_id=1184 GTF74_RS02335 WP_000649326.1 484737..485198(-) (pilA) [Vibrio cholerae O1 biovar El Tor strain E7946]
MKAYKNKQQKGFTLIELMIVVAVIGVLAAIAIPQYQNYVKKSAIGVGLANITALKTNIEDYIATEGSFPATTAGTAAGFT
RLGTVEDMGDGKIVIAPTASGALGGTIKYTFDAGVVSSSKIQLARDANGLWTCSTTVTSEIAPKGCTAGATIN

Nucleotide

• Length: 462 bp

>NTDB_id=1184 GTF74_RS02335 WP_000649326.1 484737..485198(-) (pilA) [Vibrio cholerae O1 biovar El Tor strain E7946]
ATGAAAGCGTATAAAAACAAACAACAGAAAGGTTTTACCTTAATTGAATTGATGATTGTGGTGGCAGTGATTGGTGTGTT
GGCAGCGATTGCAATTCCTCAATATCAAAATTATGTAAAAAAATCAGCTATTGGGGTTGGCTTAGCAAACATCACAGCAC
TGAAAACTAATATTGAAGATTATATTGCGACAGAAGGCTCTTTTCCTGCAACAACTGCAGGTACGGCCGCAGGATTTACA
CGCTTAGGTACTGTTGAAGACATGGGTGACGGTAAGATTGTAATTGCCCCAACAGCATCAGGTGCATTAGGAGGTACAAT
AAAATACACATTCGATGCTGGTGTAGTATCTAGTTCAAAAATCCAATTAGCAAGAGATGCCAATGGATTATGGACTTGTA
GTACCACCGTTACTAGTGAAATAGCACCAAAAGGCTGTACTGCAGGTGCAACAATTAACTAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (7-31)

• Pilin (bacterial filament) (Pilin) (42-147)

3D structure

• AlphaFold DB: A0A0X1KZH2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Vibrio cholerae C6706	100	100	1
pilA	Vibrio cholerae strain A1552	100	100	1
pilA	Pseudomonas aeruginosa PAK	42.675	100	0.447
pilA	Vibrio parahaemolyticus RIMD 2210633	41.892	100	0.446
pilA	Acinetobacter baumannii strain A118	42.667	100	0.43
pilA/pilAI	Pseudomonas stutzeri DSM 10701	39.86	100	0.41
pilA2	Legionella pneumophila str. Paris	38.462	100	0.404
pilA2	Legionella pneumophila strain ERS1305867	37.762	100	0.397
pilA	Acinetobacter nosocomialis M2	42.969	90.141	0.387
pilA/pilAII	Pseudomonas stutzeri DSM 10701	36.986	100	0.383
comP	Acinetobacter baylyi ADP1	37.063	97.279	0.361

References

• [1]: Courtney K Ellison et al. (2018) Retraction of DNA-bound type IV competence pili initiates DNA uptake during natural transformation in Vibrio cholerae. Nature Microbiology 3(7):773-780. [PMID: 29891864]