Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GTF74_RS02330 Genome accession   NZ_CP047303
Coordinates   483049..484737 (-) Length   562 a.a.
NCBI ID   WP_000957200.1    Uniprot ID   A0A0H3AHY8
Organism   Vibrio cholerae O1 biovar El Tor strain E7946     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 478049..489737
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GTF74_RS02305 (GTF74_02305) yacG 479131..479328 (-) 198 WP_000162868.1 DNA gyrase inhibitor YacG -
  GTF74_RS02310 (GTF74_02310) zapD 479470..480210 (-) 741 WP_000207198.1 cell division protein ZapD -
  GTF74_RS02315 (GTF74_02315) coaE 480242..480850 (-) 609 WP_000011557.1 dephospho-CoA kinase -
  GTF74_RS02320 (GTF74_02320) pilD 480847..481722 (-) 876 WP_000418747.1 A24 family peptidase Machinery gene
  GTF74_RS02325 (GTF74_02325) pilC 481780..483006 (-) 1227 WP_000648511.1 type II secretion system F family protein Machinery gene
  GTF74_RS02330 (GTF74_02330) pilB 483049..484737 (-) 1689 WP_000957200.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GTF74_RS02335 (GTF74_02335) pilA 484737..485198 (-) 462 WP_000649326.1 pilin Machinery gene
  GTF74_RS02340 (GTF74_02340) nadC 485443..486333 (-) 891 WP_000665274.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GTF74_RS02345 (GTF74_02345) ampD 486629..487174 (+) 546 WP_000567318.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GTF74_RS02350 (GTF74_02350) fldB 487452..487970 (-) 519 WP_000690112.1 flavodoxin FldB -
  GTF74_RS02355 (GTF74_02355) xerD 488108..489016 (+) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62150.20 Da        Isoelectric Point: 5.9053

>NTDB_id=412461 GTF74_RS02330 WP_000957200.1 483049..484737(-) (pilB) [Vibrio cholerae O1 biovar El Tor strain E7946]
MLTNLVAILRQAELISATQEQAVVTQVSASGTSVPEALLELSIFHAQELTEQLSHIFGLPETDLSRYDYANLCQQLGLRE
LITRYDALPIAKQGNLLLLAVSDPTLLQAEEEFRFATGLQVELALADHRALQAAIRRLYGRSIQGAANQGKEISQDELAN
LVKVSDDELQSIEDLSQDDSPVSRFINQVLLDAVRKGASDIHFEPYENQYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLSRDTAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYNPQQKQLYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRILNTSQINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLANMGVEPFNLASSLSLIIAQRLARRLCKHCKIAVRPSALLQSQFAFQPNE
ILYEANAAGCNECTGGYSGRVGIYEVMAFNTELAEAIMQRASIHQIERLAKANGMQTLQESGLEKLREGITSFAELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=412461 GTF74_RS02330 WP_000957200.1 483049..484737(-) (pilB) [Vibrio cholerae O1 biovar El Tor strain E7946]
ATGCTCACCAACCTTGTTGCTATCTTGCGTCAGGCTGAGTTAATCAGCGCAACGCAAGAACAAGCGGTGGTTACACAGGT
TAGCGCTTCGGGGACTTCGGTGCCCGAGGCGCTGCTTGAGTTAAGTATTTTTCACGCCCAAGAACTGACCGAACAACTGA
GCCATATTTTCGGCTTGCCGGAAACCGACCTTAGCCGCTACGACTACGCCAACTTGTGCCAACAGCTCGGGCTGCGTGAA
CTGATTACCCGCTACGATGCCTTGCCGATTGCCAAGCAAGGCAATTTATTGCTGCTTGCGGTCTCTGACCCGACCTTACT
GCAAGCCGAAGAAGAATTTCGTTTTGCCACAGGATTACAAGTTGAACTGGCACTGGCCGATCACCGCGCGCTGCAAGCCG
CGATTCGCCGTTTGTATGGCCGCTCAATTCAAGGCGCAGCCAACCAAGGGAAAGAGATCAGCCAAGATGAGCTCGCCAAT
CTGGTTAAAGTCAGTGACGACGAGCTGCAATCCATTGAAGATCTCAGCCAAGATGACTCTCCGGTTAGCCGCTTTATCAA
CCAAGTGCTGCTCGATGCGGTACGTAAAGGTGCCTCGGATATTCATTTTGAGCCGTATGAAAACCAGTATCGGATCCGCC
TGCGCTGCGATGGCATCTTGGTCGAAACTCAGCAACCGGCTAGCCATTTAAGCCGCCGTTTAGCTGCGCGGATTAAAATT
CTCTCCAAATTAGATATTGCCGAGCGCCGCTTGCCGCAAGACGGGCGGATTAAACTGCGCCTAAGCCGCGATACCGCCAT
TGATATGCGTGTTTCGACACTTCCCACTTTATGGGGAGAAAAAATCGTGCTGCGTCTGCTCGATAGCAGCGCCGCCAATC
TGGATATTGATAAGCTCGGCTATAACCCGCAGCAAAAGCAACTCTACCTCAACGCCCTGAAAAGACCGCAAGGAATGATT
TTAATGACCGGCCCCACCGGCAGCGGCAAAACCGTTTCGCTCTATACTGGGCTGCGCATTCTCAACACGTCACAGATCAA
TATCTCCACCGCGGAAGATCCGGTAGAAATTAACCTCTCTGGGATTAACCAAGTGCAAGTGCAGCCGAAAATCGGCTTTG
GCTTTGCCGAAGCGCTACGCTCGTTTCTGCGCCAAGACCCGGATGTGGTGATGGTCGGCGAAATCCGCGATCTGGAAACC
GCAGAAATCGCGGTCAAAGCCGCGCAAACCGGTCACTTAGTGCTTTCCACCCTGCACACCAATTCGGCCGCTGAAACCGT
AATTCGTTTAGCCAATATGGGGGTGGAGCCGTTTAACCTCGCGTCCTCACTCAGTTTAATCATCGCCCAACGCCTCGCGC
GCCGCCTATGTAAACACTGCAAAATCGCGGTGCGCCCTTCTGCCCTATTGCAAAGCCAATTTGCATTTCAACCCAATGAA
ATCTTGTATGAAGCGAATGCGGCGGGGTGTAACGAGTGTACGGGCGGCTATTCAGGGCGCGTTGGGATCTATGAAGTGAT
GGCGTTTAATACCGAGCTGGCGGAGGCCATTATGCAACGCGCCAGCATTCATCAAATTGAACGTTTAGCCAAAGCCAATG
GCATGCAAACGTTGCAAGAGTCCGGTCTTGAAAAGCTGCGCGAAGGCATCACCAGCTTTGCCGAGCTGCAGCGTGTGCTC
TACTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H3AHY8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

100

100

1
  pilB Vibrio parahaemolyticus RIMD 2210633

73.665

100

0.737
  pilB Vibrio campbellii strain DS40M4

73.488

100

0.735
  pilB Legionella pneumophila strain ERS1305867

50.741

96.085

0.488
  pilB Acinetobacter baylyi ADP1

50.484

91.993

0.464
  pilB Acinetobacter baumannii D1279779

50.29

91.993

0.463
  pilF Neisseria gonorrhoeae MS11

45.374

100

0.454
  pilB/pilB1 Synechocystis sp. PCC 6803

36.65

100

0.393
  pilF Thermus thermophilus HB27

40.111

96.263

0.386
  pilB Haemophilus influenzae 86-028NP

39.658

93.772

0.372
  pilB Haemophilus influenzae Rd KW20

39.279

93.772

0.368

Multiple sequence alignment