Detailed information    

experimental Experimentally validated

Overview

Name   pilB   Type   Machinery gene
Locus tag   GCO85_RS07725 Genome accession   NZ_CP048618
Coordinates   1702902..1704626 (+) Length   574 a.a.
NCBI ID   WP_011946523.1    Uniprot ID   -
Organism   Legionella pneumophila strain ERS1305867     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

Mutation of this gene caused a decrease in transformation.


Genomic Context

Location: 1697902..1709626
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GCO85_RS07705 (GCO85_07715) - 1698255..1700777 (+) 2523 WP_061636355.1 GGDEF domain-containing phosphodiesterase -
  GCO85_RS07710 (GCO85_07720) - 1700779..1701348 (-) 570 WP_011213801.1 hypoxanthine-guanine phosphoribosyltransferase -
  GCO85_RS07715 (GCO85_07725) - 1701391..1701717 (-) 327 WP_011213802.1 DUF1820 family protein -
  GCO85_RS07720 (GCO85_07730) - 1701900..1702643 (-) 744 WP_061636356.1 methyltransferase domain-containing protein -
  GCO85_RS07725 (GCO85_07735) pilB 1702902..1704626 (+) 1725 WP_011946523.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GCO85_RS07730 (GCO85_07740) pilC 1704632..1705852 (+) 1221 WP_011213805.1 type II secretion system F family protein Machinery gene
  GCO85_RS07735 (GCO85_07745) pilD 1705898..1706761 (+) 864 WP_011946525.1 A24 family peptidase Machinery gene
  GCO85_RS07740 (GCO85_07750) - 1706775..1707980 (-) 1206 WP_013101470.1 cyclic nucleotide-binding domain-containing protein -
  GCO85_RS07745 (GCO85_07755) - 1708088..1708681 (-) 594 WP_011213808.1 hypothetical protein -
  GCO85_RS07750 (GCO85_07760) - 1708697..1709152 (-) 456 WP_027226538.1 hypothetical protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62722.31 Da        Isoelectric Point: 5.5861

>NTDB_id=1251 GCO85_RS07725 WP_011946523.1 1702902..1704626(+) (pilB) [Legionella pneumophila strain ERS1305867]
MALATEEYRLQGIGQLLVLEKLLDKTKAIELHKLAAAEKISLLQYIVKNKILSAEQIALTAAQNFGVPMLDINCIDVGTI
PVNLVNEKLIKRHAMVPLFSRGTNLYLATDDPSKQASLKEIQFHTGLNTHAIVVETDKLSALIDNLLTAKESQGLSEYVE
DSGDLEGLEISADDEDQDIDTATSVTDDAPIVKFVNKILLDAIRQGASDIHFEPYEREYRIRYRQDGILHEVATPPASLS
SRITARIKVMSNLDISERRIPQDGGFKMKISKSRAIDFRVSTCPTSAGEKVVMRVLDSGAAKLGIEALGFNPVQRTNFLK
AIQRPQGMILVTGPTGSGKTVTLYTALNILNTIEVNISTAEDPVEIKVPGINQVNINPKAGLTFSGALRSFLRQDPDIIM
VGEIRDLETAEIAIKAAQTGHLVLSTLHTNSAAETLNRLVNMGIPTFNIASSVTLIIAQRLARKLCNQCKAVRDDFTNQG
LIELGFKEADLVNLKLYKAVGCEQCTSGYRGRVGLFEVLPMTKELGQLIMSGGNSLDILKLAQSEGMLTIFQSGIEKVKE
GITTIEEVNRVTVD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=1251 GCO85_RS07725 WP_011946523.1 1702902..1704626(+) (pilB) [Legionella pneumophila strain ERS1305867]
ATGGCTCTAGCTACAGAAGAATATAGATTACAGGGAATCGGCCAGCTTCTCGTGCTGGAAAAGCTGCTAGACAAAACAAA
AGCAATTGAACTACACAAGTTGGCAGCAGCAGAAAAGATATCTTTGCTGCAATACATTGTAAAAAATAAAATATTATCTG
CTGAACAAATTGCACTGACGGCTGCTCAAAATTTTGGCGTACCCATGTTGGATATTAATTGCATTGATGTGGGCACCATT
CCTGTCAATCTGGTTAATGAGAAATTAATTAAACGTCATGCCATGGTTCCTCTTTTTAGTCGCGGCACCAATTTATATCT
CGCAACAGATGATCCTAGTAAACAGGCTTCATTAAAGGAAATTCAATTTCATACCGGATTAAATACTCATGCGATAGTTG
TAGAAACAGATAAACTTAGTGCTCTGATTGATAACCTGTTAACAGCAAAGGAAAGTCAGGGCTTATCAGAGTATGTTGAA
GACTCTGGAGATCTAGAAGGTTTGGAAATAAGTGCTGATGATGAAGATCAAGATATTGATACTGCAACATCAGTAACTGA
TGATGCACCTATCGTAAAATTCGTCAATAAAATTTTATTGGATGCGATAAGGCAAGGAGCTTCTGATATACACTTTGAGC
CTTATGAACGAGAATATCGAATCAGGTACAGGCAAGATGGTATTTTACATGAGGTCGCTACCCCTCCCGCAAGCTTGTCA
TCTCGTATTACAGCACGTATCAAGGTAATGTCTAATTTGGATATATCAGAACGGCGTATCCCCCAAGATGGTGGCTTCAA
AATGAAAATTTCCAAGTCACGAGCAATTGATTTCAGAGTGAGCACATGTCCAACCTCGGCTGGGGAAAAAGTGGTTATGC
GGGTTCTTGATTCTGGTGCTGCTAAATTAGGTATTGAAGCATTAGGATTTAATCCTGTCCAGCGAACAAATTTCCTGAAA
GCAATCCAGCGCCCACAAGGGATGATTTTAGTCACTGGCCCTACTGGTAGTGGTAAAACAGTCACTTTATATACGGCTTT
AAATATATTAAATACAATTGAGGTTAATATTTCAACAGCTGAAGATCCAGTTGAAATCAAAGTGCCTGGTATCAATCAGG
TTAATATTAATCCTAAAGCTGGCCTAACCTTTTCCGGTGCATTGCGTTCGTTTCTAAGGCAAGATCCTGATATTATCATG
GTTGGTGAGATACGAGACCTTGAAACAGCAGAGATTGCTATAAAAGCTGCTCAGACAGGGCATTTGGTTCTTTCTACTCT
GCACACAAACAGTGCCGCTGAAACTTTAAATCGTTTAGTAAATATGGGAATACCTACTTTTAACATCGCGAGTTCGGTTA
CCTTAATTATTGCGCAAAGGCTGGCAAGAAAATTATGCAATCAATGTAAGGCAGTGCGTGATGACTTTACCAATCAAGGC
TTGATTGAACTAGGTTTCAAGGAAGCTGATTTGGTTAATCTTAAATTATACAAAGCAGTTGGTTGTGAGCAATGTACAAG
TGGTTATCGCGGGCGAGTTGGGTTGTTTGAGGTTTTACCGATGACAAAAGAGCTTGGCCAATTGATTATGTCAGGTGGTA
ATTCGTTGGATATATTAAAGTTAGCTCAATCAGAAGGTATGCTGACTATCTTTCAATCGGGTATAGAAAAAGTTAAGGAA
GGAATCACGACCATAGAGGAGGTCAATCGGGTAACCGTTGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-151)

Type II/IV secretion system protein (T2SSE)

(197-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

49.742

100

0.507
  pilB Acinetobacter baumannii D1279779

50.794

99.474

0.505
  pilB Vibrio cholerae strain A1552

50.741

96.085

0.488
  pilF Neisseria gonorrhoeae MS11

47.173

100

0.478
  pilB Vibrio campbellii strain DS40M4

47.756

99.287

0.474
  pilB Vibrio parahaemolyticus RIMD 2210633

48.98

96.078

0.471
  pilB Haemophilus influenzae 86-028NP

39.468

100

0.416
  pilB Haemophilus influenzae Rd KW20

39.264

100

0.414
  pilB Glaesserella parasuis strain SC1401

38.211

100

0.408
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.509

92.16

0.364
  pilF Thermus thermophilus HB27

38.848

93.728

0.364

Multiple sequence alignment    



References

[1] Léo Hardy et al. (2021) Transposon Insertion Sequencing in a Clinical Isolate of Legionella pneumophila Identifies Essential Genes and Determinants of Natural Transformation. Journal of Bacteriology 203(3):e00548-20. [PMID: 33168636]