Detailed information NTG0001018

Experimentally validated

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: ACIAD_RS01685
• Genome accession: NC_005966
• Coordinates: 354628..356340 (-)
• Length: 570 a.a.
• NCBI ID: WP_004920473.1
• Uniprot ID: -
• Organism: Acinetobacter baylyi ADP1
• Function: power the assembly of type IV pilus DNA binding and uptake

Function

pilB gene encodes a T4P assembly ATPase. PilB is suggested to power the assembly of the DNA translocator pseudopilus.

Genomic Context

Location: 349628..361340

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACIAD_RS01655 (ACIAD0356)	-	349793..350119 (+)	327	WP_004920494.1	pyrimidine/purine nucleoside phosphorylase	-
ACIAD_RS01660 (ACIAD0357)	rlmB	350219..350965 (+)	747	WP_004920490.1	23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB	-
ACIAD_RS01665 (ACIAD0358)	-	351006..351923 (+)	918	WP_004920488.1	DMT family transporter	-
ACIAD_RS01670 (ACIAD0359)	coaE	351920..352507 (-)	588	WP_004920482.1	dephospho-CoA kinase	-
ACIAD_RS01675 (ACIAD0360)	pilD	352513..353373 (-)	861	WP_004920478.1	prepilin peptidase	Machinery gene
ACIAD_RS01680 (ACIAD0361)	pilC	353373..354599 (-)	1227	WP_004920476.1	type II secretion system F family protein	Machinery gene
ACIAD_RS01685 (ACIAD0362)	pilB	354628..356340 (-)	1713	WP_004920473.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ACIAD_RS01690 (ACIAD0363)	tpiA	356605..357402 (+)	798	WP_004920470.1	triose-phosphate isomerase	-
ACIAD_RS01695 (ACIAD0364)	secG	357412..357741 (+)	330	WP_004920467.1	preprotein translocase subunit SecG	-
ACIAD_RS01715 (ACIAD0367)	rimP	358821..359345 (+)	525	WP_004920465.1	ribosome maturation factor RimP	-
ACIAD_RS01720 (ACIAD0368)	nusA	359381..360865 (+)	1485	WP_004920463.1	transcription termination factor NusA	-

Sequence

Protein

• Length: 570 a.a.
• Molecular weight: 63976.80 Da
• Isoelectric Point: 5.3446

>NTDB_id=1018 ACIAD_RS01685 WP_004920473.1 354628..356340(-) (pilB) [Acinetobacter baylyi ADP1]
MSAFTTPPKFSGFIRRLVEEGYVNAQNMQQALEKAKKFKQDIVPYLIDNFSISPLTIAEIISLEFGEPLLDLGVFDPALF
LKDKIDEKLIQKYRIMPLVHRGHVLYVATSNPTNIEAMDAIRFNSKLKVEPIIVEHDKLERLLSEHFVEETHFNFDTEEL
DLDVEVDPHTTDDDDEDDKLKDEAPIVKYINKLLIDAIRMSASDLHFEPYEKSYRVRYRVDGVLRLIATPPLQLATRLAS
RLKVMSQMDISEKRVPQDGRIKLKMSKSKTIDFRVNSLPTLFGEKIVLRILDPASAMLGIDALGYEPEQKALFMEALNKP
QGMLLITGPTGSGKTVSLYTGLNILNTEHANISTAEDPVEINLEGVNQVNVNPKVGLTFAAALRSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHLVMSTLHTNNAAETLTRLRNMGVASFNIATSVNLVIAQRLARRLCSQCKRPIQVPERSLLEMGF
TPEDLAQPEFQIFEPVGCHDCREGYKGRVGIYEVMKITPEISKIIMEDGNALEIAATAETLGFNNLRRSGLKKVMQGVTS
LQEINRVTSE

Nucleotide

• Length: 1713 bp

>NTDB_id=1018 ACIAD_RS01685 WP_004920473.1 354628..356340(-) (pilB) [Acinetobacter baylyi ADP1]
ATGTCAGCATTTACAACACCACCTAAGTTTTCGGGGTTTATTCGGCGTTTAGTCGAAGAAGGCTATGTGAATGCACAAAA
TATGCAGCAGGCTTTGGAAAAAGCAAAAAAATTTAAACAAGATATTGTGCCCTACTTGATTGATAATTTTTCAATCTCTC
CACTCACCATTGCAGAAATCATTTCTTTAGAGTTTGGAGAACCATTACTTGATCTGGGTGTTTTTGATCCCGCTTTATTT
TTAAAAGATAAAATTGATGAAAAACTGATCCAGAAATATCGAATCATGCCATTGGTGCATCGCGGGCATGTGTTGTATGT
CGCAACCAGTAATCCAACCAACATTGAGGCAATGGATGCGATTCGATTTAATAGCAAGCTCAAAGTTGAACCAATTATTG
TTGAACATGACAAATTAGAGCGATTGCTTTCAGAACATTTTGTTGAGGAAACTCATTTTAATTTTGATACAGAAGAGCTT
GATCTGGATGTTGAAGTCGATCCACATACAACGGATGACGACGATGAGGATGACAAACTTAAAGACGAAGCACCTATTGT
TAAATACATTAATAAGTTACTGATTGATGCGATACGCATGAGTGCTTCGGATTTACACTTTGAGCCCTATGAGAAAAGCT
ATCGGGTCCGTTATCGGGTAGATGGTGTACTGCGGTTGATTGCAACACCTCCCCTGCAACTGGCAACACGACTGGCCTCA
CGTTTGAAAGTCATGTCACAAATGGATATTTCGGAAAAACGTGTTCCGCAAGATGGCCGTATTAAACTCAAAATGTCTAA
AAGTAAAACCATTGATTTTCGGGTCAATTCTTTGCCGACATTATTTGGGGAAAAAATTGTTCTTCGGATTCTTGATCCAG
CCAGTGCCATGCTCGGTATTGATGCACTCGGTTATGAACCCGAGCAAAAAGCACTATTTATGGAGGCTTTAAACAAACCG
CAAGGGATGCTTCTAATTACGGGACCAACGGGTTCAGGTAAAACCGTATCTTTATATACAGGCTTAAATATACTCAATAC
CGAACATGCCAATATTTCCACAGCAGAAGATCCAGTAGAAATCAATCTCGAAGGCGTCAATCAGGTCAATGTCAATCCAA
AGGTTGGACTGACATTTGCTGCTGCATTAAGATCCTTTTTACGTCAGGATCCTGATATTATTATGGTCGGGGAAATACGT
GATCTGGAAACCGCTGAGATCGCCATTAAAGCAGCTCAAACAGGTCATTTGGTCATGTCGACGTTGCATACCAATAATGC
AGCAGAAACACTGACTCGACTCAGAAATATGGGCGTTGCTTCTTTTAATATTGCAACCTCAGTGAATCTGGTGATCGCAC
AACGTCTTGCTAGGCGTTTATGCAGCCAGTGCAAACGACCGATTCAGGTTCCAGAGCGAAGTTTGCTGGAAATGGGCTTT
ACGCCAGAAGATTTAGCTCAACCAGAATTTCAGATTTTTGAGCCTGTAGGGTGTCATGACTGTCGCGAAGGCTATAAAGG
CCGTGTGGGGATTTACGAAGTCATGAAAATTACACCAGAAATATCAAAAATTATTATGGAAGATGGTAATGCGCTAGAAA
TTGCCGCAACAGCCGAAACATTGGGGTTCAATAATTTACGTCGGTCGGGTCTGAAAAAAGTGATGCAGGGGGTCACCTCG
TTACAGGAAATTAATCGTGTAACCAGTGAATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (192-459)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (17-150)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	79.825	100	0.798
pilB	Legionella pneumophila strain ERS1305867	49.742	100	0.507
pilF	Neisseria gonorrhoeae MS11	50.178	100	0.505
pilB	Vibrio parahaemolyticus RIMD 2210633	48.115	99.287	0.478
pilB	Vibrio cholerae strain A1552	50.484	91.993	0.464
pilB	Vibrio campbellii strain DS40M4	49.903	92.157	0.46
pilB	Haemophilus influenzae Rd KW20	39.113	100	0.418
pilB	Haemophilus influenzae 86-028NP	38.105	100	0.407
pilB	Glaesserella parasuis strain SC1401	36.634	100	0.401
pilB/pilB1	Synechocystis sp. PCC 6803	34.084	100	0.372

References

• [1]: Beate Averhoff et al. (2021) Natural transformation in Gram-negative bacteria thriving in extreme environments: from genes and genomes to proteins, structures and regulation. Extremophiles : Life Under Extreme Conditions 25(5-6):425-436. [PMID: 34542714]
• [2]: Colleen G Leong et al. (2017) The role of core and accessory type IV pilus genes in natural transformation and twitching motility in the bacterium Acinetobacter baylyi. PloS One 12(8):e0182139. [PMID: 28771515]