Detailed information NTG0001366

Experimentally validated

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: NTHI_RS01950
• Genome accession: NC_007146
• Coordinates: 395024..396418 (-)
• Length: 464 a.a.
• NCBI ID: WP_011271972.1
• Uniprot ID: -
• Organism: Haemophilus influenzae 86-028NP
• Function: type IV pilus biogenesis and function DNA binding and uptake

Function

The pilABCD and comABCDEF gene clusters are operons and that the product of each gene is essential for normal pilus function. Mutants with nonpolar deletions in each of the 10 pil and com genes had an adherence defect when primary human airway cells were used as the target. These mutants were also diminished in their ability to form a biofilm in vitro and, additionally, were deficient in natural transformation.

Genomic Context

Location: 390024..401418

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NTHI_RS01915	-	390267..390473 (-)	207	WP_005631184.1	heavy-metal-associated domain-containing protein	-
NTHI_RS01920	-	390558..390764 (-)	207	WP_005631184.1	heavy-metal-associated domain-containing protein	-
NTHI_RS01925 (NTHI0403)	-	390841..391227 (+)	387	WP_005631185.1	Cu(I)-responsive transcriptional regulator	-
NTHI_RS01930 (NTHI0404)	metJ	391241..391558 (-)	318	WP_005634287.1	met regulon transcriptional regulator MetJ	-
NTHI_RS01935 (NTHI0405)	rho	391802..393064 (+)	1263	WP_005648966.1	transcription termination factor Rho	-
NTHI_RS01940 (NTHI0406)	pilD	393118..393810 (-)	693	WP_011271970.1	prepilin peptidase	Machinery gene
NTHI_RS01945 (NTHI0407)	pilC	393807..395027 (-)	1221	WP_011271971.1	type II secretion system F family protein	Machinery gene
NTHI_RS01950 (NTHI0408)	pilB	395024..396418 (-)	1395	WP_011271972.1	GspE/PulE family protein	Machinery gene
NTHI_RS01955 (NTHI0409)	pilA	396415..396864 (-)	450	WP_011271973.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	Machinery gene
NTHI_RS01960 (NTHI0411)	ampD	396979..397539 (+)	561	WP_011271974.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
NTHI_RS01965 (NTHI0412)	corC	398161..399060 (+)	900	WP_005656283.1	CNNM family magnesium/cobalt transport protein CorC	-
NTHI_RS01970 (NTHI0413)	lnt	399083..400612 (+)	1530	WP_079989936.1	apolipoprotein N-acyltransferase	-
NTHI_RS01975 (NTHI0414)	rsmE	400662..401399 (+)	738	WP_011271976.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-

Sequence

Protein

• Length: 464 a.a.
• Molecular weight: 52962.30 Da
• Isoelectric Point: 5.6248

>NTDB_id=1366 NTHI_RS01950 WP_011271972.1 395024..396418(-) (pilB) [Haemophilus influenzae 86-028NP]
MTSYALLHTQRVTAQNGEIFTISPDLWERNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPHQMQVEEQVKFYQHQETHFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFAELG
MTENQQQAFQRSLSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLLR
AFLRQDPDIIMLGEIRDEESARIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQHEIENSLLLVIAQRLVRKICPKC
GGNLINSCDCHQGYRGRIGVYQFLHWQQNGYQTDFENLRESGLEKVSQGITDEKEIERVLGKNS

Nucleotide

• Length: 1395 bp

>NTDB_id=1366 NTHI_RS01950 WP_011271972.1 395024..396418(-) (pilB) [Haemophilus influenzae 86-028NP]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAACCGCTCAAAATGGCGAGATCTTTACGATCTCGCCAGATTTATG
GGAACGCAATCAGCAGCAACAATCCTTGCTCTTGCGGTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCTTGTGAAACCATTGCGTTTATAACAGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGCACCAAATGCAAGTGGAAGAGCAAGTTAAATTCTA
TCAACATCAAGAAACCCATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGACGATTTCAATTTAAAACCACTTTTTCCGATATTCTTGATTTTCGCCTTTCAACCT
TACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCTGTAGAACTTAGCTTTGCTGAACTGGGT
ATGACCGAAAATCAGCAACAAGCATTTCAACGCTCACTTAGCCAGCCACAAGGATTAATTTTAGTAACCGGCCCCACAGG
AAGTGGGAAAAGTATCTCGCTTTACACCGCACTTCAGTGGCTAAATACGCCTGATAAACATATTATGACCGCTGAAGATC
CCATTGAAATTGAACTTGATGGTATTATTCAAAGCCAAATTAATCCGCAGATTGGATTAGATTTTAGCCGTCTATTGCGT
GCTTTTTTACGTCAAGATCCCGACATCATTATGCTAGGTGAAATTCGAGATGAAGAAAGTGCAAGGATTGCACTACGTGC
CGCTCAAACGGGACATTTGGTGCTTTCAACTTTACATACCAATGATGCAATATCTGCCATTTCTCGCTTACAACAACTCG
GTATTCAACAACATGAAATTGAAAACAGTTTACTACTCGTCATTGCACAGCGTCTTGTACGAAAAATCTGTCCAAAGTGC
GGTGGAAATTTAATAAATTCTTGTGATTGCCATCAAGGTTATCGAGGGCGAATCGGCGTGTATCAATTTCTACATTGGCA
ACAGAATGGCTATCAAACGGATTTTGAGAATTTACGAGAGAGTGGTTTGGAAAAAGTTAGCCAAGGCATAACAGATGAGA
AAGAAATTGAACGTGTGTTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (24-98)

• Type II/IV secretion system protein (T2SSE) (128-395)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Haemophilus influenzae Rd KW20	96.544	99.784	0.963
pilB	Glaesserella parasuis strain SC1401	57.456	98.915	0.568
pilB	Vibrio cholerae strain A1552	39.658	100	0.45
pilB	Vibrio campbellii strain DS40M4	40.082	100	0.422
pilB	Vibrio parahaemolyticus RIMD 2210633	39.959	100	0.416
pilB	Legionella pneumophila strain ERS1305867	39.468	100	0.416
pilB	Acinetobacter baylyi ADP1	38.105	100	0.407
pilB	Acinetobacter baumannii D1279779	38.669	100	0.401
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	37.393	100	0.377
pilF	Neisseria gonorrhoeae MS11	36.555	100	0.375
pilF	Thermus thermophilus HB27	36.134	100	0.371

References

• [1]: Michael D Carruthers et al. (2012) Biological roles of nontypeable Haemophilus influenzae type IV pilus proteins encoded by the pil and com operons. Journal of Bacteriology 194(8):1927-33. [PMID: 22328674]