Detailed information NTG0001110

Experimentally validated

Overview

• Name: pilF
• Type: Machinery gene
• Locus tag: NGFG_RS09235
• Genome accession: NC_022240
• Coordinates: 1698704..1700380 (-)
• Length: 558 a.a.
• NCBI ID: WP_003689817.1
• Uniprot ID: A0AAQ1E824
• Organism: Neisseria gonorrhoeae MS11
• Function: assembly of type IV pilus DNA binding and uptake

Function

pilF and pilD gene products are required for competence for DNA transformation. Mutants lacking the pilD gene product, a pre-pilin peptidase, were unable to process the pre-pilin subunit into pilin and thus were non-piliated. pilF mutants processed pilin but did not assemble the mature subunit.

Genomic Context

Location: 1693704..1705380

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NGFG_RS09215 (NGFG_02119)	pilG	1695621..1696853 (+)	1233	WP_003689811.1	type 4 pilus assembly protein PilG	Machinery gene
NGFG_RS09220 (NGFG_02120)	pilD	1696927..1697787 (+)	861	WP_003689814.1	prepilin peptidase	Machinery gene
NGFG_RS09225 (NGFG_02121)	coaE	1697784..1698416 (+)	633	WP_003689816.1	dephospho-CoA kinase	-
NGFG_RS09230 (NGFG_02122)	-	1698409..1698618 (+)	210	WP_003696096.1	DNA gyrase inhibitor YacG	-
NGFG_RS09235 (NGFG_02123)	pilF	1698704..1700380 (-)	1677	WP_003689817.1	type IV-A pilus assembly ATPase PilB	Machinery gene
NGFG_RS09245 (NGFG_02124)	-	1700718..1701164 (-)	447	WP_003689818.1	DUF441 domain-containing protein	-
NGFG_RS09250 (NGFG_02125)	-	1701168..1702142 (-)	975	WP_003689819.1	polyprenyl synthetase family protein	-
NGFG_RS09255 (NGFG_02126)	rplU	1702366..1702674 (+)	309	WP_003691838.1	50S ribosomal protein L21	-
NGFG_RS09260 (NGFG_02127)	rpmA	1702699..1702971 (+)	273	WP_003689821.1	50S ribosomal protein L27	-
NGFG_RS09265 (NGFG_02128)	ubiM	1703206..1704390 (+)	1185	WP_010951321.1	5-demethoxyubiquinol-8 5-hydroxylase UbiM	-
NGFG_RS09270 (NGFG_02129)	rpmG	1704579..1704734 (-)	156	WP_003689824.1	50S ribosomal protein L33	-
NGFG_RS09275 (NGFG_02130)	rpmB	1704766..1704999 (-)	234	WP_002216391.1	50S ribosomal protein L28	-

Sequence

Protein

• Length: 558 a.a.
• Molecular weight: 61863.00 Da
• Isoelectric Point: 5.5420

>NTDB_id=1110 NGFG_RS09235 WP_003689817.1 1698704..1700380(-) (pilF) [Neisseria gonorrhoeae MS11]
MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVISPKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQ
MVEFHCVPVFRRGDKVFFAVSDPTQMPQIQKTVSAAGIAVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPIAVRGQLASRIKVMSRLDISEKR
IPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMRILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSG
KTVSLYTCLNILNTESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAAQ
TGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSSCKQEVERPSASALKEVGFTDEDLAKDWKLY
RAVGCDRCRGQGYKGRAGVYEVMPISEEMQRVIMNNGTEVGILDVAYKEGMVDLRRAGILKIMQGITSLEEVTANTND

Nucleotide

• Length: 1677 bp

>NTDB_id=1110 NGFG_RS09235 WP_003689817.1 1698704..1700380(-) (pilF) [Neisseria gonorrhoeae MS11]
ATGAGCGTAGGTTTGCTGAGGATTCTGGTTCAAAACCAGGTGGTTACTGTTGAGCAGGCCGAGCATTACTACAATGAGTC
GCAGGCGGGTAAGGAAGTGTTGCCGATGCTGTTTTCAGACGGTGTCATTTCGCCCAAGTCGCTTGCGGCATTGATTGCGA
GGGTGTTCAGTTATTCGATTCTTGATTTGCGTCATTATCCGCGCCACAGGGTGCTGATGGGGGTGTTGACGGAGGAGCAG
ATGGTGGAGTTCCACTGTGTGCCGGTTTTCCGTCGGGGCGACAAAGTATTTTTTGCGGTTTCCGATCCGACCCAGATGCC
GCAAATTCAGAAAACCGTTTCTGCCGCAGGGATTGCGGTTGAGTTGGTCATTGTCGAGGATGACCAGTTGGCGGGTTTGC
TCGATTGGGTGGGTTCGCGTTCGACATCGCTGCTTCAGGAGCTTGGGGAGGGGCAAGAGGAAGAGGAAAGCCACACCCTG
TATATCGACAACGAGGAGGCAGAAGACGGCCCTGTTCCGAGGTTTATCCATAAAACTTTGTCGGATGCCTTGCGTAGCGG
GGCATCCGACATCCATTTCGAGTTTTACGAACACAATGCGCGTATCCGTTTCCGTGTGGACGGGCAGCTCCGCGAGGTGG
TTCAGCCGCCCATTGCGGTAAGGGGGCAGCTTGCTTCCCGGATTAAGGTAATGTCGCGTTTGGACATTTCCGAAAAACGG
ATACCGCAGGACGGTAGGATGCAGCTGACCTTTCAAAAGGGCGGCAAGCCTGTCGATTTCCGTGTCAGCACATTGCCGAC
GCTGTTTGGCGAAAAGGTCGTGATGCGGATTTTGAATTCCGATGCCGCGTCTTTGAACATCGACCAGCTCGGTTTTGAGC
CGTTCCAGAAAAAATTGTTGTTGGAAGCGATTCACCGTCCTTACGGGATGGTGCTGGTAACCGGCCCGACGGGTTCGGGT
AAGACGGTGTCGCTCTATACCTGTTTGAATATTTTGAATACGGAGTCGGTAAATATTGCAACGGCGGAAGACCCTGCCGA
GATTAACCTGCCGGGCATCAATCAGGTTAACGTCAATGATAAGCAGGGTCTGACTTTTGCCGCTGCTTTGAAGTCTTTCC
TGCGTCAGGACCCGGACATCATTATGGTCGGTGAGATTCGTGATTTGGAAACTGCCGATATTGCGATTAAGGCGGCACAA
ACAGGGCATATGGTGTTTTCCACACTGCACACGAATAATGCGCCGGCGACGTTGTCGCGTATGCTGAATATGGGTGTCGC
GCCGTTTAATATTGCCAGTTCGGTCAGCCTGATTATGGCGCAGCGTCTTTTACGCAGGCTGTGTTCGAGCTGCAAACAGG
AAGTGGAACGCCCGTCTGCCTCTGCTTTGAAGGAAGTCGGTTTCACCGATGAGGATCTTGCAAAAGATTGGAAACTTTAC
CGCGCCGTCGGTTGCGACCGTTGCCGGGGGCAGGGTTATAAGGGGCGTGCGGGCGTGTATGAGGTTATGCCCATCAGCGA
AGAAATGCAGCGTGTGATTATGAACAACGGTACGGAAGTGGGTATTTTGGACGTTGCCTATAAGGAGGGTATGGTGGATT
TGCGCCGGGCCGGTATTTTGAAAATTATGCAGGGCATTACTTCATTGGAAGAGGTAACGGCAAATACCAACGATTAG

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (38-139)

• Type II/IV secretion system protein (T2SSE) (178-446)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	50.088	100	0.509
pilB	Acinetobacter baylyi ADP1	50.178	100	0.505
pilB	Legionella pneumophila strain ERS1305867	47.173	100	0.478
pilB	Vibrio parahaemolyticus RIMD 2210633	45.567	100	0.461
pilB	Vibrio cholerae strain A1552	45.374	100	0.457
pilB	Vibrio campbellii strain DS40M4	43.838	100	0.446
pilB	Haemophilus influenzae Rd KW20	36.765	100	0.377
pilB	Glaesserella parasuis strain SC1401	36.421	100	0.375
pilB	Haemophilus influenzae 86-028NP	36.555	100	0.375
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.885	93.907	0.375

References

• [1]: N E Freitag et al. (1995) Characterization of the pilF-pilD pilus-assembly locus of Neisseria gonorrhoeae. Molecular Microbiology 16(3):575-86. [PMID: 7565116]