Detailed information    

experimental Experimentally validated

Overview

Name   pilG   Type   Machinery gene
Locus tag   NGFG_RS09215 Genome accession   NC_022240
Coordinates   1695621..1696853 (+) Length   410 a.a.
NCBI ID   WP_003689811.1    Uniprot ID   -
Organism   Neisseria gonorrhoeae MS11     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

Chromosomal pilG insertion mutants were devoid of pili and displayed dramatically reduced competence for transformation.


Genomic Context

Location: 1690621..1701853
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGFG_RS09200 (NGFG_02116) fabI 1691497..1692282 (-) 786 WP_002223367.1 enoyl-ACP reductase FabI -
  NGFG_RS09205 (NGFG_02117) dapD 1692437..1693258 (-) 822 WP_003689804.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -
  NGFG_RS09210 (NGFG_02118) pgi 1693468..1695111 (-) 1644 WP_003689806.1 glucose-6-phosphate isomerase -
  NGFG_RS09215 (NGFG_02119) pilG 1695621..1696853 (+) 1233 WP_003689811.1 type 4 pilus assembly protein PilG Machinery gene
  NGFG_RS09220 (NGFG_02120) pilD 1696927..1697787 (+) 861 WP_003689814.1 prepilin peptidase Machinery gene
  NGFG_RS09225 (NGFG_02121) coaE 1697784..1698416 (+) 633 WP_003689816.1 dephospho-CoA kinase -
  NGFG_RS09230 (NGFG_02122) - 1698409..1698618 (+) 210 WP_003696096.1 DNA gyrase inhibitor YacG -
  NGFG_RS09235 (NGFG_02123) pilF 1698704..1700380 (-) 1677 WP_003689817.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NGFG_RS09245 (NGFG_02124) - 1700718..1701164 (-) 447 WP_003689818.1 DUF441 domain-containing protein -

Sequence

Protein


Download         Length: 410 a.a.        Molecular weight: 45332.36 Da        Isoelectric Point: 10.0085

>NTDB_id=1112 NGFG_RS09215 WP_003689811.1 1695621..1696853(+) (pilG) [Neisseria gonorrhoeae MS11]
MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARKKLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLS
TMIKAGLPLMQAFEIVARGHGNPSMTEMLMEIRGQVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPPLTQTVMDMSDFFVSYGWMVLIALGFAI
YGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTIARWGRTTATLFAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRV
IQGLSMTSGMRATELFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIGTLLVAMYL
PLFNLGNVVA

Nucleotide


Download         Length: 1233 bp        

>NTDB_id=1112 NGFG_RS09215 WP_003689811.1 1695621..1696853(+) (pilG) [Neisseria gonorrhoeae MS11]
ATGGCTAAAAATGGGGGATTTTCTTTGTTCGCAAAGAAAGAAAAACGCTTTATCTTTGAAGGCAGGCATTCTGCCTCCGA
CAAACTGGTCAACGGCGAAGTATCCGCGTTTACCGAAGAAGAGGCACGCAAAAAACTGGCAAAACGCGGCATCCGCCCGT
TGCAGATTACCCGTGTGAAAACAAGCTCCAAGCGCAAAATCACACAAGAAGACATTACCGTTTTCACCCGCCAGCTTTCC
ACGATGATTAAAGCGGGCCTGCCGCTGATGCAGGCATTTGAAATCGTGGCGCGCGGACACGGCAACCCGTCTATGACGGA
AATGCTGATGGAAATCCGAGGCCAAGTGGAACAGGGCAGCTCGTTGAGCCGCGCATTCTCAAACCACCCGAAATATTTCG
ACCGCTTCTACTGCAACCTGGTTGCGGCGGGCGAAACGGGCGGTGTATTGGAAAGCCTGCTGGACAAATTGGCAATTTAC
AAGGAAAAAACCCAGGCCATCCGCAAAAAGGTAAAAACCGCGCTAACCTATCCGGTATCCGTCATCGCCGTCGCCATCGG
TTTGGTATTCGTGATGATGATTTTCGTACTGCCCGCCTTTAAAGAAGTTTACGCCAATATGGGCGCGGAGCTTCCCCCAC
TGACCCAAACAGTGATGGATATGTCCGACTTTTTCGTCTCATACGGCTGGATGGTGCTGATCGCACTGGGCTTTGCCATA
TACGGCTTCCTTAAATTGAAGGCGCGTTCGATTAAAATCCAACGGCGTATGGATGCCATACTGCTGCGTATGCCGATTTT
CGGAGACATTGTCCGCAAAGGGACGATTGCCCGCTGGGGCAGGACGACGGCAACGCTGTTTGCCGCCGGCGTGCCTTTGG
TCGATGTATTGGACTCCACTGCCGGCGCGGCGGGCAATTTAATCTATGAAGAAGCCACCCGGGAAATCCGTACGCGCGTC
ATCCAAGGTCTGTCTATGACTTCGGGGATGCGTGCGACGGAACTGTTCCCCAATATGATGTTGCAGATGTCCTCCATCGG
CGAGGAATCGGGTTCTTTGGACGATATGCTCAACAAAGCCGCCGAATTTTACGAAGACGAGGTGGACAATGCGGTCGGCA
GGCTGTCCGCTATGATGGAGCCGATTATTATTGTGATTTTGGGCTTGGTCATCGGTACGCTTCTGGTCGCTATGTATCTG
CCGCTGTTCAACTTGGGCAACGTGGTCGCCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(281-401)

Type II secretion system (T2SS), protein F (T2SSF)

(75-198)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria meningitidis 44/76-A

98.78

100

0.988
  pilC Acinetobacter baumannii D1279779

47.236

97.549

0.461
  pilC Pseudomonas stutzeri DSM 10701

44.665

99.506

0.444
  pilC Acinetobacter baylyi ADP1

45.363

97.794

0.444
  pilC Legionella pneumophila strain ERS1305867

44.836

97.783

0.438
  pilC Vibrio cholerae strain A1552

41.294

98.529

0.407
  pilC Vibrio campbellii strain DS40M4

39.066

100

0.391

Multiple sequence alignment    



References

[1] T Tønjum et al. (1995) Identification and characterization of pilG, a highly conserved pilus-assembly gene in pathogenic Neisseria. Molecular Microbiology 16(3):451-64. [PMID: 7565106]