Detailed information    

experimental Experimentally validated

Overview

Name   pilC   Type   Machinery gene
Locus tag   ABD1_RS01610 Genome accession   NC_020547
Coordinates   346189..347415 (-) Length   408 a.a.
NCBI ID   WP_000279215.1    Uniprot ID   A0A0H5FGB3
Organism   Acinetobacter baumannii D1279779     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

We examined the 33 knockout mutants by comparing their transformation efficiency with wild-type W068. No knockout strains were affected in growth, but 28 of these mutants were severely or completely impaired in natural transformability. These mutations were in 18 genes related to T4P (pilF, pilQ, tsaP, pilM, pilN, pilO, pilP, pilB, pilC, pilT, pilD, pilE, pilY2, pilY1, pilX, pilW, pilV, and fimU), six genes related to DNA uptake and processing (comEA, comA, comF, priA, dprA, and recA), two genes related to T2SS (xcpV and xcpW), and the crp and tonB2 genes.


Genomic Context

Location: 341189..352415
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABD1_RS01580 (ABD1_02980) - 341220..342530 (+) 1311 WP_000753285.1 tetratricopeptide repeat protein -
  ABD1_RS01585 (ABD1_02990) - 342607..342933 (+) 327 WP_000099685.1 pyrimidine/purine nucleoside phosphorylase -
  ABD1_RS01590 (ABD1_03000) rlmB 343035..343784 (+) 750 WP_001118149.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ABD1_RS01595 (ABD1_03010) - 343820..344734 (+) 915 WP_000075010.1 DMT family transporter -
  ABD1_RS01600 (ABD1_03020) coaE 344731..345327 (-) 597 WP_000874125.1 dephospho-CoA kinase -
  ABD1_RS18470 (ABD1_03030) pilD 345329..346189 (-) 861 WP_001152280.1 prepilin peptidase Machinery gene
  ABD1_RS01610 (ABD1_03040) pilC 346189..347415 (-) 1227 WP_000279215.1 type II secretion system F family protein Machinery gene
  ABD1_RS01615 (ABD1_03050) pilB 347445..349157 (-) 1713 WP_001274986.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABD1_RS01620 (ABD1_03060) tpiA 349448..350242 (+) 795 WP_000016939.1 triose-phosphate isomerase -
  ABD1_RS01625 (ABD1_03070) secG 350255..350584 (+) 330 WP_000555570.1 preprotein translocase subunit SecG -
  ABD1_RS01645 (ABD1_03080) rimP 351226..351750 (+) 525 WP_000777730.1 ribosome maturation factor RimP -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 44950.46 Da        Isoelectric Point: 9.9566

>NTDB_id=1059 ABD1_RS01610 WP_000279215.1 346189..347415(-) (pilC) [Acinetobacter baumannii D1279779]
MAVKKAQMMPTFAYEGVDRKGVKIKGELPAKNMALAKVTLRKQGVTVRNIREKRKNILEGLFKKKVTTLDITIFTRQLAT
MMKAGVPLVQGFEIVAEGLENPAMREVVLGIKGEVEGGSTFASALRKYPQHFDNLFCSLVESGEQSGALETMLDRVAIYK
EKSELLKQKIKKAMKYPATVIVVAIVVTIILMVKVVPVFQDLFASFGADLPAFTQMVVNMSKWMQEYWFIMIIAIGAVIA
AFLEAKKRSKKFRDGLDKLALKLPIFGDLVYKAIIARYSRTLATTFAAGVPLIDALESTAGATNNVIYEKAVMKIREDVA
TGQQLQFAMRISNRFPSMAIQMVAIGEESGALDSMLDKVATYYENEVDNAVDGLTSMMEPLIMAILGVLVGGLVIAMYLP
IFQMGSVV

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=1059 ABD1_RS01610 WP_000279215.1 346189..347415(-) (pilC) [Acinetobacter baumannii D1279779]
ATGGCTGTCAAAAAGGCACAAATGATGCCGACTTTTGCTTATGAAGGGGTTGACCGTAAGGGCGTAAAAATCAAGGGAGA
ACTTCCGGCTAAAAACATGGCTTTAGCCAAAGTAACCTTACGCAAACAAGGGGTGACTGTTCGAAATATACGTGAAAAAC
GTAAAAATATTCTTGAAGGTTTATTCAAGAAAAAAGTCACCACACTCGATATTACGATTTTTACTCGACAACTAGCGACC
ATGATGAAAGCTGGTGTACCACTCGTACAAGGCTTTGAAATTGTAGCTGAAGGTTTAGAAAATCCGGCCATGCGTGAGGT
TGTACTCGGTATTAAAGGCGAAGTCGAGGGTGGTAGTACTTTTGCCTCAGCTTTAAGAAAATATCCTCAACATTTTGATA
ACTTGTTTTGTTCACTTGTTGAGTCTGGTGAACAATCAGGCGCACTCGAAACGATGCTGGACCGCGTTGCAATTTACAAA
GAAAAAAGTGAATTGCTCAAGCAAAAAATCAAGAAAGCAATGAAATACCCGGCAACAGTTATTGTAGTAGCCATTGTGGT
GACCATTATTTTGATGGTTAAAGTAGTTCCCGTTTTCCAAGATTTATTTGCGTCTTTTGGAGCAGATTTACCTGCATTTA
CGCAAATGGTTGTGAATATGTCGAAATGGATGCAGGAATACTGGTTCATTATGATTATTGCGATTGGCGCAGTCATTGCT
GCCTTTCTGGAAGCAAAAAAACGCAGTAAAAAGTTTCGTGACGGCTTAGATAAACTGGCGCTTAAGTTACCTATTTTTGG
GGACTTGGTTTATAAAGCAATTATTGCCCGCTATAGCCGCACGCTTGCTACAACATTTGCCGCAGGTGTTCCGCTTATTG
ATGCATTAGAATCGACTGCTGGCGCAACCAATAACGTGATTTATGAAAAAGCTGTGATGAAAATTCGTGAAGATGTAGCA
ACGGGTCAACAGCTTCAGTTTGCTATGCGGATCTCTAATCGTTTTCCATCTATGGCTATACAAATGGTTGCAATTGGTGA
AGAATCGGGTGCACTAGACAGCATGCTCGATAAAGTTGCCACTTACTATGAAAATGAAGTGGATAATGCCGTTGATGGTT
TAACCTCTATGATGGAACCTTTAATTATGGCAATTTTAGGGGTGCTCGTAGGCGGTCTGGTAATTGCTATGTATCTTCCA
ATTTTCCAAATGGGCTCAGTTGTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(74-197)

Type II secretion system (T2SS), protein F (T2SSF)

(279-400)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H5FGB3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Acinetobacter baylyi ADP1

85.784

100

0.858
  pilC Pseudomonas stutzeri DSM 10701

59.753

100

0.598
  pilC Legionella pneumophila strain ERS1305867

53.827

99.754

0.537
  pilG Neisseria meningitidis 44/76-A

47.236

97.549

0.461
  pilG Neisseria gonorrhoeae MS11

47.236

97.549

0.461
  pilC Vibrio cholerae strain A1552

42.647

100

0.426
  pilC Vibrio campbellii strain DS40M4

41.089

99.02

0.407
  pilC Thermus thermophilus HB27

36.658

98.768

0.362

Multiple sequence alignment    



References

[1] Yuan Hu et al. (2021) Natural Transformation in Acinetobacter baumannii W068: A Genetic Analysis Reveals the Involvements of the CRP, XcpV, XcpW, TsaP, and TonB2. Frontiers in Microbiology 12:738034. [PMID: 35126321]