Detailed information    

experimental Experimentally validated

Overview

Name   pilC   Type   Machinery gene
Locus tag   PSJM300_03950 Genome accession   CP003725
Coordinates   875269..876486 (+) Length   405 a.a.
NCBI ID   AFN76868.1    Uniprot ID   -
Organism   Pseudomonas stutzeri DSM 10701     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

The pilC mutant had no pili and was defective in twitching motility.


Genomic Context

Location: 870269..881486
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSJM300_03930 - 870915..872306 (-) 1392 AFN76864.1 pilus biogenesis protein -
  PSJM300_03935 pilA/pilAII 872331..872756 (-) 426 AFN76865.1 fimbrial protein pilin: general secretion pathway protein H Regulator
  PSJM300_03940 pilA/pilAI 872824..873243 (-) 420 AFN76866.1 fimbrial protein pilin: general secretion pathway protein H Machinery gene
  PSJM300_03945 pilB 873564..875267 (+) 1704 AFN76867.1 type 4 fimbrial biogenesis protein PilB Machinery gene
  PSJM300_03950 pilC 875269..876486 (+) 1218 AFN76868.1 type 4 pilus biogenesis protein PilC Machinery gene
  PSJM300_03955 pilD 876489..877358 (+) 870 AFN76869.1 type 4 prepilin peptidase PilD Machinery gene
  PSJM300_03960 coaE 877599..878207 (+) 609 AFN76870.1 dephospho-CoA kinase -
  PSJM300_03965 - 878204..878398 (+) 195 AFN76871.1 DNA gyrase inhibitor -
  PSJM300_03970 - 878730..879926 (+) 1197 AFN76872.1 FHA domain-containing protein -
  PSJM300_03975 - 879933..880415 (+) 483 AFN76873.1 hypothetical protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44121.72 Da        Isoelectric Point: 9.8187

>NTDB_id=1198 PSJM300_03950 AFN76868.1 875269..876486(+) (pilC) [Pseudomonas stutzeri DSM 10701]
MAQKAIKNSVFTWEGLDRQGAKIKGELSGVSPALVKAQLRKQGINPQKVRKKSISLFSAGKKIKPMDIALFTRQMATMMK
AGVPLLQSFDIIGEGFDNPNMRKLVDDLKQEVAAGNSFASSLRKKPQYFDDLYCNLVDSGEQSGSLETLLDRVATYKEKT
EALKAKIKKAMNYPIAVVLVAVIVTAILLIKVVPQFQDVFANFGAELPAFTLMVIGLSEALQAWWYVVLAGFVGAAYAFK
QAHTRSEKFRNWFDRLMLKVPVVGDILYKSSVARFARTLSTTFAAGVPLVDALDSVAGATGNVVFRSATNKIKSDVSSGM
QLNFSMRTTGTFPSMAVQMTAIGEESGALDEMLSKVATFYEDEVDNMVDGLTALMEPMIMAVLGVLVGGLIIAMYLPIFQ
MGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1198 PSJM300_03950 AFN76868.1 875269..876486(+) (pilC) [Pseudomonas stutzeri DSM 10701]
ATGGCGCAGAAAGCGATTAAGAACAGTGTCTTCACCTGGGAAGGGCTCGACCGGCAGGGCGCGAAGATCAAGGGTGAGCT
GAGTGGCGTGAGCCCGGCGCTGGTCAAGGCGCAACTGCGCAAGCAGGGGATCAATCCGCAGAAGGTGCGCAAGAAGTCGA
TTTCGCTCTTCAGTGCGGGCAAGAAGATCAAGCCGATGGACATCGCGCTGTTCACTCGGCAGATGGCGACCATGATGAAG
GCAGGCGTACCACTGTTGCAGTCGTTCGACATCATAGGCGAGGGATTCGATAATCCGAACATGCGCAAGCTGGTCGATGA
CCTCAAACAGGAGGTCGCAGCTGGTAACAGCTTTGCCTCCTCGCTGCGCAAGAAGCCCCAGTATTTCGATGACCTGTATT
GCAACCTGGTGGATTCGGGGGAGCAGTCCGGTTCGCTGGAAACCCTGCTCGACCGTGTGGCGACCTATAAGGAGAAAACC
GAGGCGCTGAAGGCCAAGATCAAGAAGGCGATGAACTATCCGATTGCGGTAGTTTTGGTGGCCGTGATCGTTACTGCCAT
TCTTCTGATCAAGGTGGTTCCGCAGTTCCAAGATGTGTTCGCCAACTTCGGTGCGGAGTTGCCGGCTTTCACATTGATGG
TGATCGGGCTATCGGAGGCGCTGCAGGCGTGGTGGTACGTGGTGCTGGCAGGATTTGTTGGCGCGGCCTACGCGTTCAAA
CAGGCGCACACGCGTTCCGAGAAATTCCGCAACTGGTTCGACAGGCTGATGCTGAAGGTGCCGGTGGTGGGTGACATTCT
ATACAAATCTTCGGTAGCGCGTTTTGCTCGTACCTTGTCGACCACTTTTGCAGCCGGTGTGCCGCTGGTCGATGCACTGG
ATTCGGTCGCTGGCGCCACGGGTAACGTGGTTTTCCGCAGTGCAACCAACAAGATCAAGAGTGATGTGTCCAGCGGCATG
CAGCTCAATTTCTCCATGCGAACCACCGGAACATTCCCATCCATGGCGGTGCAGATGACCGCGATTGGCGAGGAGTCCGG
CGCGCTGGACGAGATGCTCTCCAAGGTAGCGACCTTCTATGAGGATGAAGTGGACAATATGGTCGATGGTCTTACCGCGC
TGATGGAGCCGATGATCATGGCGGTTCTCGGTGTGCTGGTGGGGGGGCTGATCATTGCGATGTACCTGCCAATCTTCCAG
ATGGGTTCTGTGGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Acinetobacter baylyi ADP1

60.688

100

0.61
  pilC Acinetobacter baumannii D1279779

59.753

100

0.598
  pilC Legionella pneumophila strain ERS1305867

54.208

99.753

0.541
  pilG Neisseria meningitidis 44/76-A

44.665

99.506

0.444
  pilG Neisseria gonorrhoeae MS11

44.665

99.506

0.444
  pilC Vibrio cholerae strain A1552

42.172

97.778

0.412
  pilC Vibrio campbellii strain DS40M4

40.909

97.778

0.4
  pilC Thermus thermophilus HB27

36.908

99.012

0.365

Multiple sequence alignment    



References

[1] Petra Meier et al. (2002) Natural transformation of Pseudomonas stutzeri by single-stranded DNA requires type IV pili, competence state and comA. FEMS Microbiology Letters 207(1):75-80. [PMID: 11886754]
[2] S Graupner et al. (2000) Type IV pilus genes pilA and pilC of Pseudomonas stutzeri are required for natural genetic transformation, and pilA can be replaced by corresponding genes from nontransformable species. Journal of Bacteriology 182(8):2184-90. [PMID: 10735861]