Detailed information NTG0001252

Experimentally validated

Overview

• Name: pilC
• Type: Machinery gene
• Locus tag: GCO85_RS07730
• Genome accession: NZ_CP048618
• Coordinates: 1704632..1705852 (+)
• Length: 406 a.a.
• NCBI ID: WP_011213805.1
• Uniprot ID: -
• Organism: Legionella pneumophila strain ERS1305867
• Function: assembly of type IV pilus DNA binding and uptake

Function

Mutation of this gene caused a decrease in transformation.

Genomic Context

Location: 1699632..1710852

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GCO85_RS07710 (GCO85_07720)	-	1700779..1701348 (-)	570	WP_011213801.1	hypoxanthine-guanine phosphoribosyltransferase	-
GCO85_RS07715 (GCO85_07725)	-	1701391..1701717 (-)	327	WP_011213802.1	DUF1820 family protein	-
GCO85_RS07720 (GCO85_07730)	-	1701900..1702643 (-)	744	WP_061636356.1	methyltransferase domain-containing protein	-
GCO85_RS07725 (GCO85_07735)	pilB	1702902..1704626 (+)	1725	WP_011946523.1	type IV-A pilus assembly ATPase PilB	Machinery gene
GCO85_RS07730 (GCO85_07740)	pilC	1704632..1705852 (+)	1221	WP_011213805.1	type II secretion system F family protein	Machinery gene
GCO85_RS07735 (GCO85_07745)	pilD	1705898..1706761 (+)	864	WP_011946525.1	A24 family peptidase	Machinery gene
GCO85_RS07740 (GCO85_07750)	-	1706775..1707980 (-)	1206	WP_013101470.1	cyclic nucleotide-binding domain-containing protein	-
GCO85_RS07745 (GCO85_07755)	-	1708088..1708681 (-)	594	WP_011213808.1	hypothetical protein	-
GCO85_RS07750 (GCO85_07760)	-	1708697..1709152 (-)	456	WP_027226538.1	hypothetical protein	-
GCO85_RS07755 (GCO85_07765)	-	1709286..1710101 (+)	816	WP_011215545.1	pyruvate, water dikinase regulatory protein	-

Sequence

Protein

• Length: 406 a.a.
• Molecular weight: 44671.88 Da
• Isoelectric Point: 9.6052

>NTDB_id=1252 GCO85_RS07730 WP_011213805.1 1704632..1705852(+) (pilC) [Legionella pneumophila strain ERS1305867]
MDKNSPPLLTFHYQGINKAGQKMEGDIQARSLAIAKADLRKQGIVTNKVVKKRKPLFDRKNKKITQADITVFSRQLATMI
ESGIPLVQAFDIVAKGQSNKRLKDLIETIKHDIETGLTLAESLIKHPLYFNELFCNLVDAGEKSGSLDIMLDKVATYKEK
IEIIKKKIKKALTYPIAVMVVALLVTAGLLIYVVPQFESLFKGFGADLPAMTRAVITMSEFMQAYWYIIFGALGGVVYSF
FYAKNHSLEFAQTIDRVMLKLPVIGPILEKAAIARFARTLSITFAAGLPLVEALKSVAGATGNIIYAKATDKIREEVATG
QQMFIAMENTHLFPNMVIQMVAIGEESGALEKMLSKVADFYEEEVNNAVDALSSLLEPIIMSILGILVGGLVVAMYLPIF
KLGSAV

Nucleotide

• Length: 1221 bp

>NTDB_id=1252 GCO85_RS07730 WP_011213805.1 1704632..1705852(+) (pilC) [Legionella pneumophila strain ERS1305867]
ATGGATAAAAATAGCCCACCCCTATTAACCTTTCATTATCAAGGCATTAATAAAGCCGGTCAAAAAATGGAGGGTGATAT
TCAAGCCAGAAGCTTGGCAATAGCTAAAGCTGATTTGCGTAAGCAGGGGATTGTTACCAATAAAGTAGTAAAAAAAAGAA
AACCTTTGTTTGATAGAAAAAATAAAAAAATCACACAAGCAGATATTACAGTGTTTAGCCGTCAATTAGCGACGATGATC
GAGTCAGGAATACCATTAGTGCAAGCGTTTGATATTGTTGCCAAAGGACAAAGCAATAAAAGACTTAAAGACTTAATAGA
AACAATTAAGCACGATATTGAAACGGGGCTAACGCTAGCAGAATCCTTAATTAAACATCCACTCTATTTTAATGAATTAT
TTTGTAATCTTGTCGATGCAGGCGAAAAATCAGGCTCATTAGATATCATGCTTGACAAGGTTGCAACGTATAAAGAAAAA
ATTGAAATCATAAAAAAGAAAATAAAAAAAGCTCTCACTTACCCAATTGCTGTTATGGTTGTCGCACTTTTGGTTACTGC
AGGATTATTAATCTATGTAGTTCCTCAATTTGAATCGTTGTTTAAAGGATTTGGAGCTGATTTGCCAGCGATGACTAGAG
CTGTGATTACTATGTCTGAATTTATGCAGGCTTATTGGTATATTATTTTTGGAGCTTTGGGTGGGGTTGTCTATAGCTTT
TTTTATGCTAAAAATCATTCTTTGGAGTTTGCACAAACCATTGACAGAGTGATGCTAAAACTCCCTGTAATTGGCCCTAT
TTTAGAGAAAGCAGCAATTGCTCGATTCGCGAGGACATTATCCATTACTTTTGCTGCTGGATTACCCCTGGTTGAAGCAT
TGAAATCTGTAGCTGGAGCGACTGGGAATATTATTTATGCCAAGGCGACTGATAAAATCAGAGAAGAAGTTGCAACAGGA
CAGCAAATGTTTATTGCTATGGAAAACACTCATTTATTTCCGAATATGGTAATTCAAATGGTAGCAATTGGTGAGGAATC
AGGTGCCCTTGAAAAAATGTTAAGCAAAGTAGCTGATTTTTATGAGGAAGAAGTAAATAATGCTGTAGATGCCCTAAGTA
GTTTATTGGAGCCGATAATTATGTCAATATTGGGTATACTGGTTGGAGGATTGGTGGTGGCTATGTATTTACCTATTTTC
AAACTGGGTTCTGCTGTTTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein F (T2SSF) (276-398)

• Type II secretion system (T2SS), protein F (T2SSF) (72-195)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilC	Pseudomonas stutzeri DSM 10701	54.208	99.753	0.541
pilC	Acinetobacter baumannii D1279779	53.827	99.754	0.537
pilC	Acinetobacter baylyi ADP1	53.086	99.754	0.53
pilG	Neisseria meningitidis 44/76-A	45.088	97.783	0.441
pilG	Neisseria gonorrhoeae MS11	44.836	97.783	0.438
pilC	Vibrio cholerae strain A1552	42	98.522	0.414
pilC	Vibrio campbellii strain DS40M4	39.163	100	0.392

References

• [1]: Léo Hardy et al. (2021) Transposon Insertion Sequencing in a Clinical Isolate of Legionella pneumophila Identifies Essential Genes and Determinants of Natural Transformation. Journal of Bacteriology 203(3):e00548-20. [PMID: 33168636]