Detailed information NTG0001402

Experimentally validated

Overview

• Name: pilC
• Type: Machinery gene
• Locus tag: DSB67_RS12670
• Genome accession: NZ_CP030788
• Coordinates: 2642760..2643983 (+)
• Length: 407 a.a.
• NCBI ID: WP_010643257.1
• Uniprot ID: -
• Organism: Vibrio campbellii strain DS40M4
• Function: type IV pilus biogenesis and function DNA binding and uptake

Function

Induction of TfoX expression resulted in upregulation of the genes required for DNA uptake and integration (comEC, comEA, comM, comF, and dprA) and pilus structure (pilABCD, pilMNOPQ, and minor pilins VC0857 to -0861) in DS40M4

Genomic Context

Location: 2637760..2648983

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DSB67_RS12645 (DSB67_12650)	ampD	2638780..2639331 (-)	552	WP_010643266.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
DSB67_RS12650 (DSB67_12655)	nadC	2639424..2640311 (+)	888	WP_010643264.1	carboxylating nicotinate-nucleotide diphosphorylase	-
DSB67_RS12660 (DSB67_12665)	pilA	2640576..2641040 (+)	465	WP_010643261.1	pilin	Machinery gene
DSB67_RS12665 (DSB67_12670)	pilB	2641040..2642725 (+)	1686	WP_010643259.1	type IV-A pilus assembly ATPase PilB	Machinery gene
DSB67_RS12670 (DSB67_12675)	pilC	2642760..2643983 (+)	1224	WP_010643257.1	type II secretion system F family protein	Machinery gene
DSB67_RS12675 (DSB67_12680)	pilD	2644056..2644925 (+)	870	WP_010643256.1	prepilin peptidase	Machinery gene
DSB67_RS12680 (DSB67_12685)	coaE	2644926..2645540 (+)	615	WP_010643253.1	dephospho-CoA kinase	-
DSB67_RS12685 (DSB67_12690)	zapD	2645568..2646308 (+)	741	WP_010643252.1	cell division protein ZapD	-
DSB67_RS12690 (DSB67_12695)	yacG	2646371..2646565 (+)	195	WP_010643249.1	DNA gyrase inhibitor YacG	-
DSB67_RS12695 (DSB67_12700)	rplS	2646902..2647255 (-)	354	WP_005379971.1	50S ribosomal protein L19	-
DSB67_RS12700 (DSB67_12705)	trmD	2647297..2648040 (-)	744	WP_005534054.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
DSB67_RS12705 (DSB67_12710)	rimM	2648068..2648616 (-)	549	WP_005534055.1	ribosome maturation factor RimM	-
DSB67_RS12710 (DSB67_12715)	rpsP	2648644..2648892 (-)	249	WP_004410028.1	30S ribosomal protein S16	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
tfoX	pilC	positive effect
tfoX	pilB	positive effect
tfoX	dprA	positive effect
tfoX	pilD	positive effect
tfoX	pilM	positive effect
tfoX	pilO	positive effect
tfoX	comM	positive effect
tfoX	pilA	positive effect
tfoX	pilQ	positive effect
tfoX	comEC	positive effect
tfoX	comEA	positive effect
tfoX	pilP	positive effect
tfoX	comF	positive effect
tfoX	pilN	positive effect

Sequence

Protein

• Length: 407 a.a.
• Molecular weight: 45003.29 Da
• Isoelectric Point: 10.3971

>NTDB_id=1402 DSB67_RS12670 WP_010643257.1 2642760..2643983(+) (pilC) [Vibrio campbellii strain DS40M4]
MKTIAPQLKNYRWKGINSSGKKTSGNVLAMTEIEVRERLDAQHIKIKKLKKGSISFITKISHRVKGKDITIFTRQIATML
MTGVPIVQALKLVSENHKKAEMKSILMSVTRAVEAGTPMSKAMRTASTHFDPLYTDLIATGEQSGNLSQVFERLATYREK
SEQLRAKVIKALIYPAMVVLVALGVSYLMLTRVIPEFEKMFTGFGADLPAFTQMVLNLSAWTQNWGPFIGLSFVSLLISG
KIMSQRSDSFRLLAARSSLRVPILGPVLSKAAIAKFSRTLATSFSAGIPILTALKTTSKTSGNMHYQLAIEEVYRDTAAG
MPMYIAMRHCHVFPELVLQMVMIGEESGRLDDMLNKIAAIYEFEVDNTVDNLSKILEPLIIVFLGVVVGGLVTAMYLPIF
NLMSVLG

Nucleotide

• Length: 1224 bp

>NTDB_id=1402 DSB67_RS12670 WP_010643257.1 2642760..2643983(+) (pilC) [Vibrio campbellii strain DS40M4]
ATGAAAACCATCGCTCCTCAACTTAAGAACTACCGTTGGAAAGGCATTAATAGCTCGGGTAAAAAAACCTCTGGAAACGT
GCTCGCCATGACCGAAATCGAGGTAAGAGAGCGCCTCGATGCTCAACACATCAAGATAAAGAAACTGAAGAAAGGCAGCA
TTTCTTTTATCACCAAGATCAGTCATCGCGTCAAAGGCAAAGACATCACGATATTTACTCGCCAAATTGCCACTATGCTA
ATGACGGGCGTACCGATTGTTCAGGCACTTAAGTTGGTTTCAGAGAACCACAAAAAAGCCGAGATGAAGTCGATTCTGAT
GAGTGTCACAAGAGCGGTAGAGGCAGGGACACCGATGTCGAAAGCCATGCGCACTGCCAGTACACATTTTGATCCGCTTT
ATACAGACCTAATCGCGACTGGTGAGCAGTCCGGTAACCTATCTCAAGTCTTTGAGCGTTTGGCAACTTATCGAGAAAAG
AGCGAGCAGTTGCGCGCAAAAGTGATTAAAGCGCTCATTTACCCAGCGATGGTGGTTTTAGTAGCGCTTGGGGTTTCCTA
TTTAATGCTGACTAGAGTTATCCCTGAGTTTGAAAAGATGTTTACAGGTTTTGGAGCCGACTTACCCGCCTTTACTCAGA
TGGTACTCAACCTCTCTGCTTGGACCCAAAACTGGGGGCCTTTTATTGGACTGAGTTTTGTGAGCCTGCTCATTTCCGGC
AAAATCATGTCGCAACGCTCCGACTCATTTCGCCTTTTAGCCGCGCGTTCGAGTTTAAGAGTCCCTATCTTGGGGCCTGT
ATTATCCAAAGCAGCCATTGCCAAGTTTAGTCGTACTTTAGCAACCAGCTTTAGTGCGGGTATTCCAATTCTCACCGCAT
TAAAAACCACCTCAAAAACCTCCGGTAATATGCACTATCAATTAGCAATTGAAGAAGTGTATCGAGACACTGCAGCTGGT
ATGCCGATGTATATTGCCATGCGTCACTGTCACGTTTTCCCTGAGCTAGTACTGCAAATGGTCATGATAGGTGAAGAGTC
TGGCCGCCTAGATGACATGCTCAACAAGATAGCCGCTATTTATGAGTTCGAAGTGGATAATACCGTTGATAATCTGAGTA
AGATTTTAGAACCATTGATCATCGTCTTTCTTGGCGTGGTAGTTGGCGGTCTAGTCACAGCAATGTACTTACCAATCTTT
AATTTGATGAGCGTATTAGGATAG

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein F (T2SSF) (276-398)

• Type II secretion system (T2SS), protein F (T2SSF) (72-195)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilC	Vibrio cholerae strain A1552	73.75	98.28	0.725
pilC	Acinetobacter baylyi ADP1	42.462	97.789	0.415
pilC	Acinetobacter baumannii D1279779	41.089	99.02	0.407
pilC	Pseudomonas stutzeri DSM 10701	40.909	97.778	0.4
pilC	Legionella pneumophila strain ERS1305867	39.163	100	0.392
pilG	Neisseria gonorrhoeae MS11	39.066	100	0.391
pilG	Neisseria meningitidis 44/76-A	38.235	100	0.383

References

• [1]: Chelsea A Simpson et al. (2019) Diversity in Natural Transformation Frequencies and Regulation across Vibrio Species. MBio 10(6):e02788-19. [PMID: 31848285]