Detailed information NTG0001396

Experimentally validated

Overview

• Name: comEC
• Type: Machinery gene
• Locus tag: DSB67_RS04755
• Genome accession: NZ_CP030788
• Coordinates: 988363..990621 (+)
• Length: 752 a.a.
• NCBI ID: WP_010649088.1
• Uniprot ID: -
• Organism: Vibrio campbellii strain DS40M4
• Function: ssDNA transport through the inner membrane DNA binding and uptake

Function

Induction of TfoX expression resulted in upregulation of the genes required for DNA uptake and integration (comEC, comEA, comM, comF, and dprA) and pilus structure (pilABCD, pilMNOPQ, and minor pilins VC0857 to -0861) in DS40M4

Genomic Context

Location: 983363..995621

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DSB67_RS04730 (DSB67_04730)	-	983654..984223 (-)	570	WP_010649097.1	PilZ domain-containing protein	-
DSB67_RS04735 (DSB67_04735)	lolC	984488..985696 (+)	1209	WP_010649096.1	lipoprotein-releasing ABC transporter permease subunit LolC	-
DSB67_RS04740 (DSB67_04740)	lolD	985689..986396 (+)	708	WP_010649094.1	lipoprotein-releasing ABC transporter ATP-binding protein LolD	-
DSB67_RS04745 (DSB67_04745)	lolE	986399..987643 (+)	1245	WP_010649092.1	lipoprotein-releasing ABC transporter permease subunit LolE	-
DSB67_RS04750 (DSB67_04750)	-	987845..988354 (-)	510	WP_010649090.1	DUF2062 domain-containing protein	-
DSB67_RS04755 (DSB67_04755)	comEC	988363..990621 (+)	2259	WP_010649088.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
DSB67_RS04760 (DSB67_04760)	msbA	990653..992404 (+)	1752	WP_010649086.1	lipid A ABC transporter ATP-binding protein/permease MsbA	-
DSB67_RS04765 (DSB67_04765)	lpxK	992410..993417 (+)	1008	WP_010649084.1	tetraacyldisaccharide 4'-kinase	-
DSB67_RS04770 (DSB67_04770)	-	993398..993577 (+)	180	WP_005378451.1	Trm112 family protein	-
DSB67_RS04775 (DSB67_04775)	kdsB	993577..994335 (+)	759	WP_010649083.1	3-deoxy-manno-octulosonate cytidylyltransferase	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
tfoX	comEC	positive effect
tfoX	pilB	positive effect
tfoX	dprA	positive effect
tfoX	pilC	positive effect
tfoX	pilD	positive effect
tfoX	pilM	positive effect
tfoX	pilO	positive effect
tfoX	comM	positive effect
tfoX	pilA	positive effect
tfoX	pilQ	positive effect
tfoX	comEA	positive effect
tfoX	pilP	positive effect
tfoX	comF	positive effect
tfoX	pilN	positive effect

Sequence

Protein

• Length: 752 a.a.
• Molecular weight: 84197.41 Da
• Isoelectric Point: 9.7067

>NTDB_id=1396 DSB67_RS04755 WP_010649088.1 988363..990621(+) (comEC) [Vibrio campbellii strain DS40M4]
MTLLEKSLTLALFVASVISSAWWPTMPDWRWLLLGIIATGSIIKLRRGLLSIGVIWGFMVVIIHGNVMEHQRQALFRAGG
NITINGKVDSPFTQISHGYEGIAQINQVNSQNLLPFLKPKIRLITAFPLPVNSEFTTQVTIKPILGLKNEAGFDAEKQAV
GKGIVARAVTPDDAKWLIRTYSSFRQQIITAVDTHIAELDHFPLISALAFSDRSLLAKQDWQSLRDSGLLHLVSISGLHI
GMAFAFGMSLGFVIRLLFPKFVHLPSAVGLLAALVYAWLADFSLPTTRAFSVCVIYLVLKAALVHWSPWRVLLLAVSIQL
FIEPFASFTMSFWLSYLSVVAVLLAVNVVQQRRGNRQTKLSSLLKIQLVLTLLIVPISGAFFSGTSLVSIAYNLVFIPWF
GFLVVPLMFLALIVTPISFQLAGMLWQLVDWVLLPLTWSLQFAVGSWHFVSLPLTLTILAVAICGLLRRFLNRGSLVTLV
MLVTGVALFYERTTNSWRVDVLDVGHGLAVLIEKHGKVALYDTGKAWNSGSIAQQVISPVLHRRGFGTIDMFIISHADSD
HAGGRQYIEQHFSPAQNFSSQNYADYQSCIAGERWYWQALYFEVLWPPKQVNRAYNPHSCVVRVVDKESGFKLLLTGDIE
AVSEWILMREPNKLESDVMLVPHHGSKSSSNPKFVQIVSPTLAIASLAKSNQWGMPAENVLSAYQEANAYWLDTGSHGQV
SVFIERDNWYFEAKRSETFEPWYRQMLRKRVE

Nucleotide

• Length: 2259 bp

>NTDB_id=1396 DSB67_RS04755 WP_010649088.1 988363..990621(+) (comEC) [Vibrio campbellii strain DS40M4]
ATGACTCTCTTAGAAAAAAGTTTGACCTTGGCGTTATTTGTAGCGAGCGTTATATCGTCTGCGTGGTGGCCGACGATGCC
TGATTGGCGATGGTTGCTGCTGGGAATAATTGCCACTGGCTCGATTATCAAATTACGTCGTGGCTTATTGAGCATAGGCG
TAATTTGGGGCTTTATGGTTGTCATTATCCACGGCAATGTTATGGAGCATCAAAGACAAGCCCTGTTTCGAGCAGGGGGG
AATATTACCATAAATGGCAAAGTTGACAGCCCTTTTACGCAAATAAGTCACGGATATGAAGGAATTGCGCAGATCAATCA
GGTGAATTCTCAAAACCTGTTACCTTTTCTTAAACCGAAAATCCGATTGATAACCGCTTTCCCACTGCCCGTTAACAGTG
AGTTCACTACTCAGGTGACGATCAAACCGATTCTGGGTTTGAAGAATGAGGCGGGGTTTGATGCGGAAAAGCAAGCGGTA
GGTAAGGGTATTGTCGCTAGAGCTGTCACACCTGATGACGCGAAATGGTTGATTCGTACATACTCTTCATTTCGACAGCA
AATCATTACGGCCGTTGATACTCATATTGCTGAACTTGACCATTTCCCTTTAATCAGTGCATTAGCGTTTAGCGACCGCT
CCCTGCTTGCTAAACAGGATTGGCAGTCTCTGCGAGATAGCGGGCTGCTGCATTTGGTCTCAATCTCTGGTTTGCATATT
GGAATGGCGTTTGCGTTTGGCATGAGTTTGGGCTTTGTCATTCGGCTTCTATTCCCCAAATTTGTTCACCTTCCTTCCGC
TGTCGGTTTATTGGCAGCGCTCGTTTATGCGTGGTTGGCCGATTTTTCTTTACCTACAACCCGTGCTTTCTCGGTTTGTG
TCATTTATCTCGTACTTAAAGCGGCGTTGGTTCATTGGAGCCCTTGGCGAGTGTTATTGCTCGCAGTCTCGATACAGCTT
TTCATTGAGCCTTTTGCTTCATTCACGATGAGCTTCTGGTTGTCTTATCTGTCCGTGGTTGCCGTGCTTTTAGCCGTGAA
TGTGGTTCAACAGCGACGCGGCAATAGGCAAACGAAGCTGAGTTCACTACTAAAAATACAGTTAGTACTTACTTTGCTTA
TCGTGCCAATCAGCGGTGCGTTTTTCTCTGGAACAAGTTTGGTATCCATCGCTTATAACCTAGTTTTCATTCCTTGGTTT
GGTTTTTTGGTCGTCCCTTTGATGTTCCTAGCGCTGATTGTAACCCCAATTTCTTTTCAGTTGGCTGGTATGCTTTGGCA
ACTGGTGGATTGGGTGTTGTTGCCACTGACATGGTCATTACAGTTTGCGGTCGGGAGTTGGCATTTTGTAAGTTTGCCTT
TGACCTTAACGATACTCGCAGTTGCCATCTGTGGATTATTGAGACGTTTTTTGAATCGGGGCTCGCTCGTTACGTTAGTC
ATGCTAGTAACGGGTGTCGCCCTATTTTATGAGCGTACAACAAATTCTTGGCGAGTGGACGTGTTAGATGTAGGGCATGG
TCTTGCGGTATTAATTGAGAAGCATGGAAAGGTGGCGCTTTATGACACAGGCAAAGCTTGGAACTCAGGGAGCATAGCTC
AGCAAGTTATCTCTCCCGTATTACATCGCAGAGGTTTTGGCACCATTGATATGTTCATTATCAGTCATGCTGATTCGGAT
CATGCTGGAGGTAGGCAATATATAGAGCAGCACTTTTCGCCAGCGCAGAACTTCAGCAGTCAAAACTACGCTGACTACCA
ATCTTGTATTGCGGGTGAACGCTGGTATTGGCAGGCGCTTTATTTTGAAGTCCTATGGCCACCTAAGCAGGTAAACCGTG
CTTATAATCCTCATTCGTGTGTTGTTCGAGTTGTGGATAAGGAATCGGGTTTTAAATTGCTTTTGACTGGCGATATTGAA
GCCGTCAGTGAGTGGATACTTATGCGTGAACCCAACAAGCTAGAGAGTGATGTTATGTTGGTTCCTCATCATGGTAGCAA
GAGCTCTTCGAATCCAAAATTTGTTCAAATTGTCTCTCCAACCTTGGCGATAGCCTCGTTAGCAAAAAGTAATCAATGGG
GCATGCCTGCTGAAAATGTTTTATCGGCTTATCAGGAAGCAAATGCCTACTGGCTGGATACGGGAAGCCATGGGCAGGTA
TCGGTGTTTATTGAGCGAGACAATTGGTACTTTGAAGCTAAACGTAGTGAGACATTTGAGCCTTGGTATAGGCAGATGCT
GCGTAAGAGAGTAGAATAA

Domains

Predicted by InterproScan.

• Metallo-beta-lactamase superfamily (Lactamase_B) (504-573)

• Competence protein (Competence) (208-466)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC	Vibrio parahaemolyticus RIMD 2210633	66.755	100	0.668
comEC	Vibrio cholerae strain A1552	40.957	100	0.412

References

• [1]: Chelsea A Simpson et al. (2019) Diversity in Natural Transformation Frequencies and Regulation across Vibrio Species. MBio 10(6):e02788-19. [PMID: 31848285]