Detailed information NTG0001295

Experimentally validated

Overview

• Name: comEC
• Type: Machinery gene
• Locus tag: VP_RS04795
• Genome accession: NC_004603
• Coordinates: 1023025..1025283 (+)
• Length: 752 a.a.
• NCBI ID: WP_005481856.1
• Uniprot ID: Q87R17
• Organism: Vibrio parahaemolyticus RIMD 2210633
• Function: ssDNA transport through the inner membrane DNA binding and uptake

Function

The ΔtfoX or ΔcytR mutant showed significant downregulation in pilA, pilB, comEA, comEC, and qstR.

Genomic Context

Location: 1018025..1030283

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VP_RS04770 (VP0976)	-	1018313..1018882 (-)	570	WP_005481874.1	PilZ domain-containing protein	-
VP_RS04775 (VP0977)	lolC	1019147..1020355 (+)	1209	WP_029787805.1	lipoprotein-releasing ABC transporter permease subunit LolC	-
VP_RS04780 (VP0978)	lolD	1020348..1021055 (+)	708	WP_005481886.1	lipoprotein-releasing ABC transporter ATP-binding protein LolD	-
VP_RS04785 (VP0979)	lolE	1021058..1022302 (+)	1245	WP_005481897.1	lipoprotein-releasing ABC transporter permease subunit LolE	-
VP_RS04790 (VP0980)	-	1022507..1023016 (-)	510	WP_005481854.1	DUF2062 domain-containing protein	-
VP_RS04795 (VP0981)	comEC	1023025..1025283 (+)	2259	WP_005481856.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
VP_RS04800 (VP0982)	msbA	1025315..1027063 (+)	1749	WP_005456206.1	lipid A ABC transporter ATP-binding protein/permease MsbA	-
VP_RS04805 (VP0983)	lpxK	1027069..1028076 (+)	1008	WP_005456276.1	tetraacyldisaccharide 4'-kinase	-
VP_RS04810	-	1028057..1028236 (+)	180	WP_005378451.1	Trm112 family protein	-
VP_RS04815 (VP0984)	kdsB	1028236..1028991 (+)	756	WP_005456221.1	3-deoxy-manno-octulosonate cytidylyltransferase	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
tfoX	comEC	positive effect
tfoX	qstR	positive effect
cytR	qstR	positive effect
cytR	comEC	positive effect
tfoX	pilB	positive effect
cytR	pilB	positive effect
tfoX	comEA	positive effect
cytR	comEA	positive effect
tfoX	pilA	positive effect
cytR	pilA	positive effect

Sequence

Protein

• Length: 752 a.a.
• Molecular weight: 84699.17 Da
• Isoelectric Point: 9.5661

>NTDB_id=1295 VP_RS04795 WP_005481856.1 1023025..1025283(+) (comEC) [Vibrio parahaemolyticus RIMD 2210633]
MTLLEKSWTLALFVASVISSAWWPTMPDWRWLLLGIITTGSIIKLRRGLISIGVIVGFMVVIVHGNIMEYQRQALFQAGE
NSTIIGRVDSSFTQISHGYEGVVAIKQVNSHTLLPFLKPKVRLITPFPLAVNSEFTTNVLIKPIIGLRNEAGFDAEKQSM
GSGVVARAVVTKDSYWVIRTSSSWREAIIQTVERDISRLEHFALIKALAFADRTGLTKEDWQSLRDSGLLHLVSISGLHI
GMALTFGLALGGLIRLAMPRYWFLPSVSGLAFAIVYAWLADFSLPTTRAVSVCIIYIALKYWLVHWSPWRVLLLAVALQL
FFQPFASFSLSFWLSYLSVGAVLFAVNTVQDSKEGRLGKLRILLLTQLILSLLIVPISGYFFSGFSWSSLVYNLVFIPWF
GFVVVPIMFAALIASLLFPMLATVLWYLLDIFLVPLSWSVRYAIGTWQPISAEWTFVIAVVSAVLVSRHVMPRYVWMFVC
VIVVMTGLFPKQYNQTWRIDVLDVGHGLAVLVEKEGRVLLYDTGKAWQNGSIAEQVITPVLHRRGYSSVDTMILSHADND
HAGGRKVIEQYFSPKHKLSSQSFLHYQPCIAAEKWKWQGLNMEVLWPPKPVVRAYNPHSCVISLEDPSTGFKMLFTGDIE
AISEWILLREPEKLRSDVMLVPHHGSKSSSNPKFINVVEPSLAIASTAKLNQWGMPAPEVVQAYTDSGVSWLDTGSDGQI
TILLDGNNWRFESKRRETIEPWYRQMLRNRVE

Nucleotide

• Length: 2259 bp

>NTDB_id=1295 VP_RS04795 WP_005481856.1 1023025..1025283(+) (comEC) [Vibrio parahaemolyticus RIMD 2210633]
ATGACTCTCTTAGAAAAAAGTTGGACCTTGGCGTTATTTGTCGCGAGCGTAATTTCGTCTGCATGGTGGCCGACGATGCC
GGATTGGCGTTGGTTGCTGCTGGGAATAATTACCACTGGCTCAATAATTAAATTACGTCGAGGCTTAATTAGCATAGGCG
TAATTGTGGGCTTTATGGTTGTCATCGTCCACGGCAATATTATGGAGTATCAGAGACAAGCCCTTTTTCAAGCAGGTGAG
AATAGTACCATAATTGGTAGAGTTGACAGCTCTTTTACGCAAATAAGTCACGGATATGAAGGTGTCGTAGCGATAAAACA
AGTGAATTCTCACACTCTGTTACCTTTTCTTAAACCTAAAGTCCGCCTTATAACGCCATTCCCACTAGCTGTTAACAGTG
AGTTTACGACTAACGTTTTGATTAAGCCCATTATCGGCCTCAGAAATGAAGCGGGTTTCGACGCAGAAAAGCAGTCAATG
GGAAGTGGTGTTGTTGCAAGGGCGGTCGTTACTAAAGATTCATACTGGGTCATTCGTACTTCTTCGTCTTGGCGCGAAGC
TATTATTCAAACTGTTGAGCGTGATATTTCTCGCCTTGAGCATTTTGCTCTAATCAAAGCACTGGCTTTTGCTGATCGCA
CTGGGCTTACCAAAGAGGATTGGCAGTCTCTGCGTGACAGCGGACTACTGCATCTTGTATCGATTTCTGGTTTACACATT
GGGATGGCACTGACGTTTGGGCTCGCCCTTGGTGGGCTTATTCGGCTTGCTATGCCGCGATATTGGTTTCTGCCATCAGT
TAGTGGGTTGGCGTTTGCCATTGTTTACGCTTGGCTTGCTGATTTTTCTTTGCCGACGACCCGAGCCGTTTCTGTTTGTA
TCATCTATATTGCTTTGAAGTATTGGCTTGTTCATTGGAGCCCATGGCGCGTACTGTTACTGGCCGTGGCATTGCAACTT
TTCTTCCAACCTTTTGCTTCTTTTAGTTTGAGTTTTTGGCTGTCTTATTTATCCGTTGGTGCCGTTTTGTTTGCGGTTAA
CACAGTGCAAGACTCGAAGGAAGGTCGTTTGGGAAAGTTGCGGATACTTCTGTTGACTCAACTGATACTGAGCTTATTGA
TTGTCCCGATCAGTGGCTATTTTTTCTCTGGATTTAGCTGGTCTTCTTTAGTCTACAATTTGGTTTTCATTCCTTGGTTT
GGCTTTGTTGTCGTTCCAATCATGTTTGCTGCTTTAATTGCGTCATTGCTCTTTCCTATGTTGGCGACGGTCCTATGGTA
TTTGCTTGACATATTCCTGGTTCCACTAAGTTGGTCGGTTCGATATGCCATAGGAACTTGGCAACCCATTAGTGCCGAGT
GGACATTTGTTATTGCTGTAGTGAGTGCCGTGCTCGTTTCAAGACATGTTATGCCTCGTTACGTTTGGATGTTTGTCTGT
GTAATTGTTGTGATGACCGGGTTGTTTCCTAAGCAATATAACCAAACTTGGCGTATTGATGTGCTTGATGTCGGGCATGG
GTTGGCGGTGCTGGTCGAAAAAGAAGGGAGAGTTTTACTCTATGATACGGGCAAGGCTTGGCAAAACGGCAGTATAGCTG
AGCAAGTGATTACGCCAGTACTGCACCGCAGAGGTTACTCAAGTGTCGATACGATGATTTTAAGTCATGCTGATAATGAC
CATGCTGGTGGCCGAAAAGTGATAGAACAGTACTTTTCACCTAAACACAAACTAAGCAGCCAGAGCTTTTTACATTATCA
GCCTTGTATTGCTGCTGAGAAATGGAAGTGGCAAGGGTTGAACATGGAGGTACTTTGGCCTCCTAAACCTGTAGTACGAG
CATACAACCCCCACTCTTGTGTCATCAGTCTGGAAGACCCTAGTACAGGTTTTAAAATGTTGTTTACTGGTGATATCGAA
GCCATCAGCGAATGGATACTGCTTCGAGAGCCAGAAAAGCTGCGCAGTGATGTAATGCTAGTGCCGCATCATGGAAGCAA
AAGCTCTTCTAACCCTAAGTTTATCAATGTTGTTGAGCCTAGCTTGGCTATTGCTTCAACGGCAAAACTAAACCAGTGGG
GAATGCCCGCACCTGAAGTCGTTCAAGCCTATACCGACAGTGGTGTTAGTTGGTTAGACACTGGAAGTGATGGCCAAATA
ACAATCTTACTTGATGGCAATAACTGGCGTTTTGAAAGTAAACGTCGTGAGACAATTGAGCCTTGGTATAGGCAGATGCT
GCGTAACCGAGTAGAATAA

Domains

Predicted by InterproScan.

• Competence protein (Competence) (208-465)

• Metallo-beta-lactamase superfamily (Lactamase_B) (504-677)

3D structure

• AlphaFold DB: Q87R17

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC	Vibrio campbellii strain DS40M4	66.755	100	0.668
comEC	Vibrio cholerae strain A1552	41.215	100	0.418

References

• [1]: Anusuya Debnath et al. (2022) Regulators of natural competence in Vibrio parahaemolyticus. Brazilian Journal of Microbiology : [publication of The Brazilian Society For Microbiology] 53(3):1491-1499. [PMID: 35761009]