Detailed information NTG0001292

Experimentally validated

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: VP_RS12235
• Genome accession: NC_004603
• Coordinates: 2663235..2663654 (+)
• Length: 139 a.a.
• NCBI ID: WP_005479693.1
• Uniprot ID: Q87LT8
• Organism: Vibrio parahaemolyticus RIMD 2210633
• Function: assembly of type IV pilus DNA binding and uptake

Function

The ΔtfoX or ΔcytR mutant showed significant downregulation in pilA, pilB, comEA, comEC, and qstR.

Genomic Context

Location: 2658235..2668654

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VP_RS12220 (VP2520)	pdhR	2660268..2661035 (-)	768	WP_005462576.1	pyruvate dehydrogenase complex transcriptional repressor PdhR	-
VP_RS12225 (VP2521)	ampD	2661441..2661992 (-)	552	WP_005479703.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
VP_RS12230 (VP2522)	nadC	2662085..2662972 (+)	888	WP_005479699.1	carboxylating nicotinate-nucleotide diphosphorylase	-
VP_RS12235 (VP2523)	pilA	2663235..2663654 (+)	420	WP_005479693.1	pilin	Machinery gene
VP_RS12240 (VP2524)	pilB	2663664..2665349 (+)	1686	WP_005479695.1	type IV-A pilus assembly ATPase PilB	Machinery gene
VP_RS12245 (VP2525)	pilC	2665373..2666596 (+)	1224	WP_005479682.1	type II secretion system F family protein	Machinery gene
VP_RS12250 (VP2526)	pilD	2666661..2667530 (+)	870	WP_005490359.1	A24 family peptidase	Machinery gene
VP_RS12255 (VP2527)	coaE	2667531..2668145 (+)	615	WP_005480887.1	dephospho-CoA kinase	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
tfoX	pilA	positive effect
tfoX	qstR	positive effect
cytR	qstR	positive effect
tfoX	comEC	positive effect
cytR	comEC	positive effect
tfoX	pilB	positive effect
cytR	pilB	positive effect
tfoX	comEA	positive effect
cytR	comEA	positive effect
cytR	pilA	positive effect

Sequence

Protein

• Length: 139 a.a.
• Molecular weight: 14180.19 Da
• Isoelectric Point: 4.4684

>NTDB_id=1292 VP_RS12235 WP_005479693.1 2663235..2663654(+) (pilA) [Vibrio parahaemolyticus RIMD 2210633]
MKHSKQKKQQGFTLIELMIVVAIIGVLAAAAIPAYQNYVTRSEVTSGLATVKALITPAELHYQENGIAAAATLAQLGVDV
AANDLGAIDSALAGGSPTLTFTFDADSSIDGGILTFTRDAATGWSCLPTAPAIAVDGCS

Nucleotide

• Length: 420 bp

>NTDB_id=1292 VP_RS12235 WP_005479693.1 2663235..2663654(+) (pilA) [Vibrio parahaemolyticus RIMD 2210633]
ATGAAACACAGTAAACAGAAAAAACAACAGGGTTTCACTCTGATTGAGTTAATGATTGTGGTAGCGATTATCGGCGTTTT
GGCTGCAGCTGCTATCCCTGCTTATCAAAATTATGTGACTCGCTCTGAAGTTACATCAGGGTTAGCAACCGTTAAAGCTC
TGATCACACCGGCTGAGCTGCATTACCAAGAAAATGGTATCGCGGCGGCTGCAACATTAGCCCAATTGGGAGTTGATGTT
GCAGCTAATGACTTAGGAGCTATTGATTCTGCCCTTGCAGGTGGTAGTCCAACTCTGACATTTACCTTCGATGCAGACAG
TTCTATCGACGGTGGGATACTAACGTTCACAAGAGACGCTGCAACTGGTTGGTCATGTTTACCTACTGCACCAGCAATTG
CAGTCGATGGTTGCAGTTAA

Domains

Predicted by InterproScan.

• Pilin (bacterial filament) (Pilin) (40-129)

• Prokaryotic N-terminal methylation motif (N_methyl) (7-31)

3D structure

• AlphaFold DB: Q87LT8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Pseudomonas aeruginosa PAK	48.507	96.403	0.468
pilA	Vibrio cholerae C6706	41.892	100	0.446
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	41.892	100	0.446
pilA	Vibrio cholerae strain A1552	41.892	100	0.446
pilA/pilAI	Pseudomonas stutzeri DSM 10701	35.862	100	0.374
pilA2	Legionella pneumophila str. Paris	38.462	95.588	0.368
pilA2	Legionella pneumophila strain ERS1305867	38.462	95.588	0.368
comF	Acinetobacter baylyi ADP1	36.691	100	0.367

References

• [1]: Anusuya Debnath et al. (2022) Regulators of natural competence in Vibrio parahaemolyticus. Brazilian Journal of Microbiology : [publication of The Brazilian Society For Microbiology] 53(3):1491-1499. [PMID: 35761009]