Detailed information    

experimental Experimentally validated

Overview

Name   pilA   Type   Machinery gene
Locus tag   ABDM36_RS02330 Genome accession   NZ_CP157384
Coordinates   484746..485207 (-) Length   153 a.a.
NCBI ID   WP_000649326.1    Uniprot ID   A0A0X1KZH2
Organism   Vibrio cholerae C6706     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

PilA is the major pilin subunit of Tfp.


Genomic Context

Location: 479746..490207
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABDM36_RS02310 coaE 480251..480859 (-) 609 WP_000011557.1 dephospho-CoA kinase -
  ABDM36_RS02315 pilD 480856..481731 (-) 876 WP_000418747.1 prepilin peptidase Machinery gene
  ABDM36_RS02320 pilC 481789..483015 (-) 1227 WP_000648511.1 type II secretion system F family protein Machinery gene
  ABDM36_RS02325 pilB 483058..484746 (-) 1689 WP_000957200.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABDM36_RS02330 pilA 484746..485207 (-) 462 WP_000649326.1 pilin Machinery gene
  ABDM36_RS02335 nadC 485452..486342 (-) 891 WP_000665274.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ABDM36_RS02340 ampD 486638..487183 (+) 546 WP_000567318.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ABDM36_RS02345 fldB 487461..487979 (-) 519 WP_000690112.1 flavodoxin FldB -
  ABDM36_RS02350 xerD 488117..489025 (+) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  ABDM36_RS02355 dsbC 489042..489794 (+) 753 WP_000115055.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  cytR pilA positive effect
  cytR comEA positive effect
  hapR comEA positive effect
  hapR pilA positive effect
  luxO hapR positive effect

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 15722.14 Da        Isoelectric Point: 8.9860

>NTDB_id=1160 ABDM36_RS02330 WP_000649326.1 484746..485207(-) (pilA) [Vibrio cholerae C6706]
MKAYKNKQQKGFTLIELMIVVAVIGVLAAIAIPQYQNYVKKSAIGVGLANITALKTNIEDYIATEGSFPATTAGTAAGFT
RLGTVEDMGDGKIVIAPTASGALGGTIKYTFDAGVVSSSKIQLARDANGLWTCSTTVTSEIAPKGCTAGATIN

Nucleotide


Download         Length: 462 bp        

>NTDB_id=1160 ABDM36_RS02330 WP_000649326.1 484746..485207(-) (pilA) [Vibrio cholerae C6706]
ATGAAAGCGTATAAAAACAAACAACAGAAAGGTTTTACCTTAATTGAATTGATGATTGTGGTGGCAGTGATTGGTGTGTT
GGCAGCGATTGCAATTCCTCAATATCAAAATTATGTAAAAAAATCAGCTATTGGGGTTGGCTTAGCAAACATCACAGCAC
TGAAAACTAATATTGAAGATTATATTGCGACAGAAGGCTCTTTTCCTGCAACAACTGCAGGTACGGCCGCAGGATTTACA
CGCTTAGGTACTGTTGAAGACATGGGTGACGGTAAGATTGTAATTGCCCCAACAGCATCAGGTGCATTAGGAGGTACAAT
AAAATACACATTCGATGCTGGTGTAGTATCTAGTTCAAAAATCCAATTAGCAAGAGATGCCAATGGATTATGGACTTGTA
GTACCACCGTTACTAGTGAAATAGCACCAAAAGGCTGTACTGCAGGTGCAACAATTAACTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0X1KZH2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae strain A1552

100

100

1
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

100

100

1
  pilA Pseudomonas aeruginosa PAK

42.675

100

0.447
  pilA Vibrio parahaemolyticus RIMD 2210633

41.892

100

0.446
  pilA Acinetobacter baumannii strain A118

42.667

100

0.43
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.86

100

0.41
  pilA2 Legionella pneumophila str. Paris

38.462

100

0.404
  pilA2 Legionella pneumophila strain ERS1305867

37.762

100

0.397
  pilA Acinetobacter nosocomialis M2

42.969

90.141

0.387
  pilA/pilAII Pseudomonas stutzeri DSM 10701

36.986

100

0.383
  comP Acinetobacter baylyi ADP1

37.063

97.279

0.361

Multiple sequence alignment    



References

[1] David W Adams et al. (2019) DNA-uptake pili of Vibrio cholerae are required for chitin colonization and capable of kin recognition via sequence-specific self-interaction. Nature Microbiology 4(9):1545-1557. [PMID: 31182799]
[2] Mirella Lo Scrudato et al. (2013) A transcriptional regulator linking quorum sensing and chitin induction to render Vibrio cholerae naturally transformable. Nucleic Acids Research 41(6):3644-58. [PMID: 23382174]
[3] Patrick Seitz et al. (2013) DNA-uptake machinery of naturally competent Vibrio cholerae. Proceedings of The National Academy of Sciences of The United States of America 110(44):17987-92. [PMID: 24127573]
[4] Mirella Lo Scrudato et al. (2012) The regulatory network of natural competence and transformation of Vibrio cholerae. PLoS Genetics 8(6):e1002778. [PMID: 22737089]
[5] Karin L Meibom et al. (2005) Chitin induces natural competence in Vibrio cholerae. Science (New York, N.Y.) 310(5755):1824-7. [PMID: 16357262]
[6] K J Fullner et al. (1999) Genetic characterization of a new type IV-A pilus gene cluster found in both classical and El Tor biotypes of Vibrio cholerae. Infection And Immunity 67(3):1393-404. [PMID: 10024587]