Detailed information    

experimental Experimentally validated

Overview

Name   pilA   Type   Machinery gene
Locus tag   FDQ49_RS11140 Genome accession   NZ_CP040105
Coordinates   2367600..2368028 (+) Length   142 a.a.
NCBI ID   WP_022575996.1    Uniprot ID   -
Organism   Acinetobacter nosocomialis M2     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

We constructed strains containing mutations in the pilA, pilD, or pilT gene and then determined the transformation efficiency of each strain. The transformation efficiency for all mutant strains was below the level of detection in our assay.


Genomic Context

Location: 2362600..2373028
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FDQ49_RS11120 (FDQ49_11120) - 2363991..2364878 (+) 888 WP_022575999.1 metal-dependent hydrolase -
  FDQ49_RS11125 (FDQ49_11125) - 2365012..2365602 (+) 591 WP_002051712.1 LemA family protein -
  FDQ49_RS11130 (FDQ49_11130) - 2365624..2366706 (+) 1083 WP_022575998.1 YgcG family protein -
  FDQ49_RS11135 (FDQ49_11135) - 2366700..2367260 (+) 561 WP_022575997.1 TPM domain-containing protein -
  FDQ49_RS11140 (FDQ49_11140) pilA 2367600..2368028 (+) 429 WP_022575996.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  FDQ49_RS11145 (FDQ49_11145) - 2368082..2369392 (+) 1311 WP_022575995.1 O-antigen ligase -
  FDQ49_RS11150 (FDQ49_11150) - 2369503..2371131 (+) 1629 WP_022575994.1 PglL family O-oligosaccharyltransferase -
  FDQ49_RS11155 (FDQ49_11155) bfr 2371172..2371636 (-) 465 WP_000678123.1 bacterioferritin -
  FDQ49_RS11160 (FDQ49_11160) - 2371881..2372075 (-) 195 WP_000289091.1 bacterioferritin-associated ferredoxin -
  FDQ49_RS11165 (FDQ49_11165) - 2372295..2372678 (-) 384 WP_022575993.1 RidA family protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 14572.69 Da        Isoelectric Point: 6.2126

>NTDB_id=1079 FDQ49_RS11140 WP_022575996.1 2367600..2368028(+) (pilA) [Acinetobacter nosocomialis M2]
MNAQKGFTLIELMIVVAIIGILAAIAIPAYQNYIAKSQASEAFTLADGLKTTINTNLQAGTCFAGGATAVTAADKVSGKY
GDAEIGGTAPNCTITYTFKSSGVSNKLTSTKIVMNVSETGILTKNSGTDTPVELLPQSFVAS

Nucleotide


Download         Length: 429 bp        

>NTDB_id=1079 FDQ49_RS11140 WP_022575996.1 2367600..2368028(+) (pilA) [Acinetobacter nosocomialis M2]
ATGAATGCACAAAAAGGTTTTACATTAATTGAACTTATGATCGTTGTAGCGATTATTGGTATTTTGGCAGCGATCGCGAT
TCCTGCTTATCAAAACTATATTGCTAAATCTCAAGCATCTGAAGCATTTACTTTAGCAGATGGTTTGAAAACGACAATTA
ATACTAATTTACAAGCTGGAACTTGTTTTGCAGGTGGTGCTACTGCAGTGACAGCAGCTGATAAAGTAAGTGGGAAATAT
GGTGATGCGGAGATTGGTGGAACTGCTCCGAACTGTACTATTACTTATACATTTAAATCATCAGGTGTATCAAATAAACT
TACATCTACAAAGATTGTAATGAATGTAAGTGAAACAGGTATTCTAACAAAGAATAGTGGTACAGATACACCAGTTGAAT
TACTTCCTCAATCATTTGTAGCATCGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

45.89

100

0.472
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.969

90.141

0.387
  pilA Vibrio cholerae C6706

42.969

90.141

0.387
  pilA Vibrio cholerae strain A1552

42.969

90.141

0.387
  pilA Pseudomonas aeruginosa PAK

45.763

83.099

0.38
  comP Acinetobacter baylyi ADP1

40

91.837

0.367

Multiple sequence alignment    



References

[1] Christian M Harding et al. (2013) Acinetobacter baumannii strain M2 produces type IV pili which play a role in natural transformation and twitching motility but not surface-associated motility. MBio 4(4):e00360-13. [PMID: 23919995]