Detailed information    

experimental Experimentally validated

Overview

Name   pilA   Type   Machinery gene
Locus tag   A1552VC_RS11065 Genome accession   NZ_CP028894
Coordinates   2373306..2373767 (+) Length   153 a.a.
NCBI ID   WP_000649326.1    Uniprot ID   A0A0X1KZH2
Organism   Vibrio cholerae strain A1552     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

PilA is the major pilin subunit of Tfp.


Genomic Context

Location: 2368306..2378767
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A1552VC_RS11040 (A1552VC_02181) dsbC 2368719..2369471 (-) 753 WP_000115055.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
  A1552VC_RS11045 (A1552VC_02182) xerD 2369488..2370396 (-) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  A1552VC_RS11050 (A1552VC_02183) fldB 2370534..2371052 (+) 519 WP_000690112.1 flavodoxin FldB -
  A1552VC_RS11055 (A1552VC_02184) ampD 2371330..2371875 (-) 546 WP_000567318.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  A1552VC_RS11060 (A1552VC_02185) nadC 2372171..2373061 (+) 891 WP_000665274.1 carboxylating nicotinate-nucleotide diphosphorylase -
  A1552VC_RS11065 (A1552VC_02186) pilA 2373306..2373767 (+) 462 WP_000649326.1 pilin Machinery gene
  A1552VC_RS11070 (A1552VC_02187) pilB 2373767..2375455 (+) 1689 WP_000957200.1 type IV-A pilus assembly ATPase PilB Machinery gene
  A1552VC_RS11075 (A1552VC_02188) pilC 2375498..2376724 (+) 1227 WP_000648511.1 type II secretion system F family protein Machinery gene
  A1552VC_RS11080 (A1552VC_02189) pilD 2376782..2377657 (+) 876 WP_000418747.1 A24 family peptidase Machinery gene
  A1552VC_RS11085 (A1552VC_02190) coaE 2377654..2378262 (+) 609 WP_000011557.1 dephospho-CoA kinase -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  tfoX pilA positive effect
  tfoX pilO positive effect
  tfoX pilB positive effect
  tfoX pilP positive effect
  tfoX pilD positive effect
  tfoX dprA positive effect
  tfoX pilM positive effect
  tfoX pilN positive effect
  tfoX comEA positive effect
  hapR comEA positive effect
  crp comEA positive effect
  qstR comEA positive effect
  tfoX pilQ positive effect
  tfoX qstR positive effect
  qstR comEC positive effect
  qstR comF positive effect
  qstR comM positive effect
  crp qstR positive effect
  hapR qstR positive effect
  tfoX comEC positive effect
  hapR comEC positive effect
  tfoX pilC positive effect
  chiS tfoX positive effect
  hapR dns negative effect

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 15722.14 Da        Isoelectric Point: 8.9860

>NTDB_id=1167 A1552VC_RS11065 WP_000649326.1 2373306..2373767(+) (pilA) [Vibrio cholerae strain A1552]
MKAYKNKQQKGFTLIELMIVVAVIGVLAAIAIPQYQNYVKKSAIGVGLANITALKTNIEDYIATEGSFPATTAGTAAGFT
RLGTVEDMGDGKIVIAPTASGALGGTIKYTFDAGVVSSSKIQLARDANGLWTCSTTVTSEIAPKGCTAGATIN

Nucleotide


Download         Length: 462 bp        

>NTDB_id=1167 A1552VC_RS11065 WP_000649326.1 2373306..2373767(+) (pilA) [Vibrio cholerae strain A1552]
ATGAAAGCGTATAAAAACAAACAACAGAAAGGTTTTACCTTAATTGAATTGATGATTGTGGTGGCAGTGATTGGTGTGTT
GGCAGCGATTGCAATTCCTCAATATCAAAATTATGTAAAAAAATCAGCTATTGGGGTTGGCTTAGCAAACATCACAGCAC
TGAAAACTAATATTGAAGATTATATTGCGACAGAAGGCTCTTTTCCTGCAACAACTGCAGGTACGGCCGCAGGATTTACA
CGCTTAGGTACTGTTGAAGACATGGGTGACGGTAAGATTGTAATTGCCCCAACAGCATCAGGTGCATTAGGAGGTACAAT
AAAATACACATTCGATGCTGGTGTAGTATCTAGTTCAAAAATCCAATTAGCAAGAGATGCCAATGGATTATGGACTTGTA
GTACCACCGTTACTAGTGAAATAGCACCAAAAGGCTGTACTGCAGGTGCAACAATTAACTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0X1KZH2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae C6706

100

100

1
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

100

100

1
  pilA Pseudomonas aeruginosa PAK

42.675

100

0.447
  pilA Vibrio parahaemolyticus RIMD 2210633

41.892

100

0.446
  pilA Acinetobacter baumannii strain A118

42.667

100

0.43
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.86

100

0.41
  pilA2 Legionella pneumophila str. Paris

38.462

100

0.404
  pilA2 Legionella pneumophila strain ERS1305867

37.762

100

0.397
  pilA Acinetobacter nosocomialis M2

42.969

90.141

0.387
  pilA/pilAII Pseudomonas stutzeri DSM 10701

36.986

100

0.383
  comP Acinetobacter baylyi ADP1

37.063

97.279

0.361

Multiple sequence alignment    



References

[1] David W Adams et al. (2019) DNA-uptake pili of Vibrio cholerae are required for chitin colonization and capable of kin recognition via sequence-specific self-interaction. Nature Microbiology 4(9):1545-1557. [PMID: 31182799]
[2] Mirella Lo Scrudato et al. (2013) A transcriptional regulator linking quorum sensing and chitin induction to render Vibrio cholerae naturally transformable. Nucleic Acids Research 41(6):3644-58. [PMID: 23382174]
[3] Patrick Seitz et al. (2013) DNA-uptake machinery of naturally competent Vibrio cholerae. Proceedings of The National Academy of Sciences of The United States of America 110(44):17987-92. [PMID: 24127573]
[4] Mirella Lo Scrudato et al. (2012) The regulatory network of natural competence and transformation of Vibrio cholerae. PLoS Genetics 8(6):e1002778. [PMID: 22737089]
[5] Karin L Meibom et al. (2005) Chitin induces natural competence in Vibrio cholerae. Science (New York, N.Y.) 310(5755):1824-7. [PMID: 16357262]
[6] K J Fullner et al. (1999) Genetic characterization of a new type IV-A pilus gene cluster found in both classical and El Tor biotypes of Vibrio cholerae. Infection And Immunity 67(3):1393-404. [PMID: 10024587]