Detailed information    

experimental Experimentally validated

Overview

Name   comM   Type   Machinery gene
Locus tag   A1552VC_RS00115 Genome accession   NZ_CP028894
Coordinates   22658..24181 (+) Length   507 a.a.
NCBI ID   WP_000521788.1    Uniprot ID   Q9KVV6
Organism   Vibrio cholerae strain A1552     
Function   ssDNA binding   
DNA processing

Function

In vivo, we found that ComM was required for efficient comigration of linked genetic markers in Vibrio cholerae and Acinetobacter baylyi. In vitro, we showed that purified ComM binds ssDNA, oligomerizes into a hexameric ring, and has bidirectional helicase and branch migration activity.


Genomic Context

Location: 17658..29181
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A1552VC_RS00100 (A1552VC_00020) ilvE 19232..20170 (-) 939 WP_000201999.1 branched-chain-amino-acid transaminase -
  A1552VC_RS00105 (A1552VC_00021) ilvM 20182..20466 (-) 285 WP_001190199.1 acetolactate synthase 2 small subunit -
  A1552VC_RS00110 (A1552VC_00022) ilvG 20478..22124 (-) 1647 WP_000145255.1 acetolactate synthase 2 catalytic subunit -
  A1552VC_RS00115 (A1552VC_00023) comM 22658..24181 (+) 1524 WP_000521788.1 YifB family Mg chelatase-like AAA ATPase Machinery gene
  A1552VC_RS00120 (A1552VC_00024) - 24271..25155 (+) 885 WP_000881907.1 acyltransferase -
  A1552VC_RS00125 (A1552VC_00025) - 25233..25835 (-) 603 WP_000732279.1 thiol:disulfide interchange protein DsbA/DsbL -
  A1552VC_RS00130 (A1552VC_00026) - 25866..26852 (-) 987 WP_000195208.1 serine/threonine protein kinase -
  A1552VC_RS00135 (A1552VC_00027) ccoG 26900..28324 (-) 1425 WP_000076153.1 cytochrome c oxidase accessory protein CcoG -
  A1552VC_RS00145 (A1552VC_00028) - 28741..29007 (-) 267 WP_000649654.1 YihD family protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  qstR comM positive effect
  qstR comEC positive effect
  tfoX comEC positive effect
  hapR comEC positive effect
  qstR comF positive effect
  qstR comEA positive effect
  tfoX comEA positive effect
  hapR comEA positive effect
  crp comEA positive effect
  crp qstR positive effect
  tfoX qstR positive effect
  tfoX pilO positive effect
  tfoX pilB positive effect
  tfoX pilP positive effect
  tfoX pilA positive effect
  tfoX pilD positive effect
  tfoX dprA positive effect
  tfoX pilM positive effect
  tfoX pilN positive effect
  tfoX pilQ positive effect
  tfoX pilC positive effect
  chiS tfoX positive effect
  hapR qstR positive effect
  hapR dns negative effect

Sequence

Protein


Download         Length: 507 a.a.        Molecular weight: 55077.16 Da        Isoelectric Point: 8.3602

>NTDB_id=1153 A1552VC_RS00115 WP_000521788.1 22658..24181(+) (comM) [Vibrio cholerae strain A1552]
MGLAIIHSRASIGVQAPPVTVEVHISNGMPGFTLVGLPETTVKESRDRVRSAIINSRFEFPAKRITVNLAPADLPKEGGR
FDLPIALGILAASDQIARNKLESYEFIGELALSGEIRGVKGVLPAALAANQVERCLVVPHSNGDQAALVGVERHKSAQSL
LEVCADLCGQQTLSLFQSSPSVQQVSQTRDLQDIIGQQQGKRALEIAAAGNHNLLFLGPPGTGKTMLASRLCDLLPEMSD
EEAMETASIASLTQQEINQHNWKLRPFRAPHHSSSMAALVGGGTIPRPGEISLAHNGLLFLDEMPEFERKVLDSLREPLE
SGEIVISRAQGKTRFPARFQLVGALNPSPTGYYEGSQARANPQSILRYLSRLSGPLLDRFDMSIEIPALPKGTLANGGDR
GESTAAVRQRVWVARERMLARSGKVNALLQSREIEQYCPLLKADAEFLESALHRLGLSIRAYHRIIKVARTIADLQGEAQ
IARPHLAEALGYRAMDRLLKQLSAQNV

Nucleotide


Download         Length: 1524 bp        

>NTDB_id=1153 A1552VC_RS00115 WP_000521788.1 22658..24181(+) (comM) [Vibrio cholerae strain A1552]
ATGGGACTTGCGATCATTCATAGCCGCGCCAGTATTGGCGTGCAGGCACCTCCGGTCACGGTTGAGGTTCACATCAGTAA
CGGGATGCCGGGGTTTACCTTGGTAGGATTGCCAGAAACCACAGTAAAAGAGTCGCGCGATCGGGTGCGCAGCGCGATCA
TCAATTCTCGCTTTGAGTTTCCAGCGAAGCGCATTACGGTCAATTTAGCGCCAGCCGATTTACCCAAAGAGGGGGGACGT
TTTGATTTGCCGATTGCACTTGGCATTTTGGCGGCCTCGGATCAAATTGCGCGAAATAAGCTCGAAAGTTACGAATTTAT
CGGCGAATTGGCGCTATCGGGCGAAATTCGTGGCGTGAAAGGGGTTTTGCCTGCCGCGCTCGCGGCTAACCAAGTGGAGC
GCTGTTTGGTGGTGCCGCACAGTAATGGTGATCAGGCGGCTTTGGTCGGGGTTGAGCGGCATAAGTCGGCGCAAAGCTTA
CTCGAAGTGTGCGCAGACTTGTGTGGTCAGCAAACTCTATCTTTGTTTCAAAGTTCGCCCAGTGTGCAACAAGTAAGTCA
GACCCGCGATCTGCAAGACATCATAGGTCAGCAGCAGGGAAAACGAGCTTTGGAAATCGCTGCGGCTGGCAATCACAACC
TATTGTTTCTCGGCCCTCCGGGGACGGGTAAAACCATGCTCGCTTCACGGCTGTGCGATTTACTGCCAGAAATGAGTGAT
GAAGAGGCAATGGAAACAGCTTCGATTGCCTCTCTTACGCAGCAAGAGATTAACCAACATAACTGGAAGTTACGCCCTTT
TCGTGCACCGCACCATTCAAGCTCAATGGCGGCTTTGGTCGGGGGCGGCACCATTCCGCGCCCCGGTGAAATTTCTCTCG
CCCATAATGGACTACTGTTTTTGGACGAAATGCCGGAGTTTGAGCGTAAAGTGCTTGATTCACTGCGTGAACCGCTGGAA
TCGGGTGAGATCGTGATTTCGCGCGCGCAGGGGAAAACCCGTTTTCCAGCACGCTTTCAATTGGTCGGCGCGTTAAATCC
AAGCCCCACGGGCTACTACGAAGGCAGTCAGGCGCGTGCCAATCCGCAGAGTATTTTGCGCTATTTGAGTCGGCTTTCGG
GCCCTTTGTTGGATCGCTTTGATATGTCGATTGAGATCCCAGCGTTACCCAAAGGTACGCTCGCCAATGGGGGCGATCGT
GGTGAATCGACGGCGGCTGTGCGCCAGCGAGTGTGGGTAGCGCGTGAGCGTATGTTGGCGCGATCGGGCAAAGTGAATGC
TTTGCTGCAAAGTCGAGAAATTGAACAGTATTGCCCTTTGCTTAAAGCGGATGCGGAATTTCTAGAAAGCGCGCTGCATC
GCTTGGGTTTATCAATTCGGGCTTACCATCGCATCATTAAAGTGGCGCGAACCATAGCGGATTTGCAAGGTGAAGCGCAG
ATCGCTCGTCCACATTTGGCCGAGGCGTTGGGCTATCGAGCCATGGATCGCTTACTCAAACAGCTCAGTGCGCAGAACGT
CTAG

Domains

Predicted by InterproScan.

Magnesium chelatase, subunit ChlI (Mg_chelatase)

(190-391)

Magnesium chelatase, subunit ChlI C-terminal (Mg_chelatase_C)

(401-493)

Subunit ChlI of Mg-chelatase (ChlI)

(21-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9KVV6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Vibrio campbellii strain DS40M4

85.01

100

0.85
  comM Haemophilus influenzae Rd KW20

67.258

100

0.673
  comM Glaesserella parasuis strain SC1401

64.147

100

0.653
  comM Legionella pneumophila strain ERS1305867

50.806

98.608

0.501
  comM Legionella pneumophila str. Paris

50.806

98.608

0.501
  RA0C_RS07335 Riemerella anatipestifer ATCC 11845 = DSM 15868

43.222

100

0.434

Multiple sequence alignment    



References

[1] Triana N Dalia et al. (2025) DprA recruits ComM to facilitate recombination during natural transformation in Gram-negative bacteria. Proceedings of The National Academy of Sciences of The United States of America 122(15):e2421764122. [PMID: 40215278]
[2] Thomas M Nero et al. (2018) ComM is a hexameric helicase that promotes branch migration during natural transformation in diverse Gram-negative species. Nucleic Acids Research 46(12):6099-6111. [PMID: 29722872]
[3] Milena Jaskólska et al. (2018) QstR-dependent regulation of natural competence and type VI secretion in Vibrio cholerae. Nucleic Acids Research 46(20):10619-10634. [PMID: 30102403]