Detailed information    

experimental Experimentally validated

Overview

Name   dns   Type   Auxiliary factor
Locus tag   A1552VC_RS01200 Genome accession   NZ_CP028894
Coordinates   226971..227666 (-) Length   231 a.a.
NCBI ID   WP_000972596.1    Uniprot ID   A0A0H6U7M6
Organism   Vibrio cholerae strain A1552     
Function   degradation of extracellular DNA   
Defense system

Function

In the absence of Dns, transformation frequencies are significantly higher than in its presence. At low cell density, the absence of HapR relieves dns repression, leading to the degradation of free DNA and to the abrogation of the transformation phenotype.


Genomic Context

Location: 221971..232666
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A1552VC_RS01175 (A1552VC_00229) tyrS 222712..223992 (+) 1281 WP_001088155.1 tyrosine--tRNA ligase -
  A1552VC_RS01180 (A1552VC_00230) ruvX 224030..224452 (-) 423 WP_000091869.1 Holliday junction resolvase RuvX -
  A1552VC_RS01185 (A1552VC_00231) - 224533..225096 (-) 564 WP_001054768.1 YqgE/AlgH family protein -
  A1552VC_RS01190 (A1552VC_00232) gshB 225132..226088 (-) 957 WP_000593313.1 glutathione synthase -
  A1552VC_RS01195 (A1552VC_00233) rsmE 226102..226833 (-) 732 WP_001222501.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  A1552VC_RS01200 (A1552VC_00234) dns 226971..227666 (-) 696 WP_000972596.1 extracellular deoxyribonuclease Dns Auxiliary factor
  A1552VC_RS01205 (A1552VC_00235) - 227794..228282 (-) 489 WP_000251279.1 SprT family zinc-dependent metalloprotease -
  A1552VC_RS01210 (A1552VC_00236) metK 228404..229561 (-) 1158 WP_000985180.1 methionine adenosyltransferase -
  A1552VC_RS01215 (A1552VC_00237) tkt 229926..231923 (+) 1998 WP_000098688.1 transketolase -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  hapR dns negative effect
  hapR comEA positive effect
  tfoX comEA positive effect
  tfoX pilO positive effect
  tfoX pilB positive effect
  tfoX pilP positive effect
  tfoX pilA positive effect
  tfoX pilD positive effect
  tfoX dprA positive effect
  tfoX pilM positive effect
  tfoX pilN positive effect
  crp comEA positive effect
  qstR comEA positive effect
  tfoX pilQ positive effect
  tfoX qstR positive effect
  qstR comEC positive effect
  qstR comF positive effect
  qstR comM positive effect
  crp qstR positive effect
  hapR qstR positive effect
  tfoX comEC positive effect
  hapR comEC positive effect
  tfoX pilC positive effect
  chiS tfoX positive effect

Sequence

Protein


Download         Length: 231 a.a.        Molecular weight: 26713.15 Da        Isoelectric Point: 8.2936

>NTDB_id=1148 A1552VC_RS01200 WP_000972596.1 226971..227666(-) (dns) [Vibrio cholerae strain A1552]
MMIFRFVTTLAASLPLLTFAAPISFSHAKNEAVKIYRDHPVSFYCGCEIRWQGKKGIPDLESCGYQVRKNENRASRIEWE
HVVPAWQFGHQLQCWQQGGRKNCTRTSPEFNQMEADLHNLTPAIGEVNGDRSNFSFSQWNGVDGVTYGQCEMQVNFKERT
AMPPERARGAIARTYLYMSEQYGLRLSKAQSQLMQAWNNQYPVSEWECVRDQRIEKVQGNSNRFVREQCPN

Nucleotide


Download         Length: 696 bp        

>NTDB_id=1148 A1552VC_RS01200 WP_000972596.1 226971..227666(-) (dns) [Vibrio cholerae strain A1552]
ATGATGATTTTTCGTTTCGTTACCACGCTTGCTGCTAGCTTACCTCTGCTCACCTTTGCCGCCCCCATCTCTTTTAGTCA
TGCCAAAAATGAAGCGGTGAAGATTTATCGTGATCATCCGGTCTCTTTTTACTGTGGCTGCGAGATTCGCTGGCAAGGCA
AAAAAGGCATCCCGGATCTGGAGAGTTGCGGCTATCAAGTGCGTAAAAATGAGAATCGTGCTTCGCGGATTGAATGGGAA
CATGTGGTGCCGGCTTGGCAATTTGGTCATCAGTTGCAATGCTGGCAACAAGGTGGCCGTAAAAACTGCACTCGTACTAG
CCCTGAGTTCAATCAGATGGAAGCCGATCTGCACAACCTCACTCCGGCGATTGGTGAAGTGAATGGCGATCGCTCCAACT
TCAGTTTCAGTCAGTGGAATGGCGTTGATGGCGTCACCTATGGCCAATGTGAAATGCAAGTAAACTTCAAAGAGCGCACT
GCAATGCCGCCAGAGCGTGCACGCGGGGCGATTGCGCGTACTTATCTGTACATGAGTGAGCAGTACGGTTTGCGACTTTC
CAAAGCGCAAAGCCAATTAATGCAGGCATGGAACAATCAGTATCCGGTCAGCGAATGGGAATGTGTGCGTGACCAAAGAA
TTGAAAAAGTGCAAGGCAACTCGAACCGCTTTGTGCGTGAGCAATGCCCGAACTGA

Domains

Predicted by InterproScan.

Endonuclease I (Endonuclease_1)

(30-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H6U7M6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dns Vibrio parahaemolyticus RIMD 2210633

72.247

98.268

0.71
  dns Aliivibrio fischeri ES114

67.241

100

0.675
  ideA Vibrio cholerae O1 str. 2010EL-1786

51.556

99.119

0.511
  dns Campylobacter jejuni RM1221

39.45

97.758

0.386

Multiple sequence alignment    



References

[1] Mirella Lo Scrudato et al. (2012) The regulatory network of natural competence and transformation of Vibrio cholerae. PLoS Genetics 8(6):e1002778. [PMID: 22737089]
[2] Gaia Suckow et al. (2011) Quorum sensing contributes to natural transformation of Vibrio cholerae in a species-specific manner. Journal of Bacteriology 193(18):4914-24. [PMID: 21784943]
[3] Melanie Blokesch et al. (2008) The extracellular nuclease Dns and its role in natural transformation of Vibrio cholerae. Journal of Bacteriology 190(21):7232-40. [PMID: 18757542]