Detailed information    

experimental Experimentally validated

Overview

Name   crp   Type   Regulator
Locus tag   A1552VC_RS12025 Genome accession   NZ_CP028894
Coordinates   2558373..2559005 (+) Length   210 a.a.
NCBI ID   WP_000242749.1    Uniprot ID   A0A6G8D3R0
Organism   Vibrio cholerae strain A1552     
Function   promote expression of competence genes   
Competence regulation

Function

CRP is a cAMP receptor protein. cAMP plus CRP play a role in increasing competence gene expression. Deletion of putative CRP-S sites upstream of comEA and qstR significantly impaired natural transformability.


Genomic Context

Location: 2553373..2564005
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A1552VC_RS12000 (A1552VC_02369) - 2553439..2555355 (+) 1917 WP_000627678.1 ABC transporter ATP-binding protein -
  A1552VC_RS12005 (A1552VC_02370) - 2555356..2555841 (+) 486 WP_001278735.1 TIGR02444 family protein -
  A1552VC_RS12010 (A1552VC_02371) - 2555838..2556827 (+) 990 WP_000627574.1 hydrolase -
  A1552VC_RS12015 (A1552VC_02372) - 2556955..2557164 (+) 210 WP_000586562.1 YheU family protein -
  A1552VC_RS12020 (A1552VC_02373) - 2557261..2558130 (+) 870 WP_001274669.1 phosphoribulokinase -
  A1552VC_RS12025 (A1552VC_02374) crp 2558373..2559005 (+) 633 WP_000242749.1 cAMP-activated global transcriptional regulator CRP Regulator
  A1552VC_RS12030 (A1552VC_02375) - 2559084..2559875 (-) 792 WP_000189091.1 DUF1338 domain-containing protein -
  A1552VC_RS12035 (A1552VC_02376) astD 2560018..2561475 (-) 1458 WP_000150128.1 succinylglutamate-semialdehyde dehydrogenase -
  A1552VC_RS12040 (A1552VC_02377) astA 2561485..2562504 (-) 1020 WP_000962042.1 arginine N-succinyltransferase -
  A1552VC_RS12045 (A1552VC_02378) - 2562644..2563855 (-) 1212 WP_000215482.1 aspartate aminotransferase family protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  crp comEA positive effect
  tfoX comEA positive effect
  tfoX pilO positive effect
  tfoX pilB positive effect
  tfoX pilP positive effect
  tfoX pilA positive effect
  tfoX pilD positive effect
  tfoX dprA positive effect
  tfoX pilM positive effect
  tfoX pilN positive effect
  tfoX pilQ positive effect
  tfoX qstR positive effect
  tfoX comEC positive effect
  tfoX pilC positive effect
  chiS tfoX positive effect
  hapR comEA positive effect
  hapR qstR positive effect
  hapR dns negative effect
  hapR comEC positive effect
  crp qstR positive effect
  qstR comEA positive effect
  qstR comEC positive effect
  qstR comF positive effect
  qstR comM positive effect

Sequence

Protein


Download         Length: 210 a.a.        Molecular weight: 23635.45 Da        Isoelectric Point: 8.2784

>NTDB_id=1145 A1552VC_RS12025 WP_000242749.1 2558373..2559005(+) (crp) [Vibrio cholerae strain A1552]
MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHAGEKAETLYYIVKGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEG
QERTAWVRAKTPCEVAEISFKKFRQLIQVNPDILMRLSGQMARRLQVTSQKVGDLAFLDVTGRIAQTLLNLARQPDAMTH
PDGMQIKITRQEIGQIVGCSRETVGRILKMLEEQNLISAHGKTIVVYGTR

Nucleotide


Download         Length: 633 bp        

>NTDB_id=1145 A1552VC_RS12025 WP_000242749.1 2558373..2559005(+) (crp) [Vibrio cholerae strain A1552]
ATGGTTCTAGGTAAACCTCAAACCGATCCAACACTAGAGTGGTTTCTTTCACATTGTCACATTCATAAGTACCCATCAAA
AAGCACACTGATCCACGCGGGTGAGAAAGCGGAAACGCTGTACTACATCGTAAAAGGTTCAGTTGCGGTACTGATCAAAG
ATGAAGAAGGTAAAGAGATGATTCTCTCTTACCTGAACCAAGGTGATTTTATCGGTGAGCTTGGCTTGTTTGAAGAAGGC
CAAGAGCGTACAGCTTGGGTTCGTGCTAAAACACCTTGTGAAGTGGCTGAAATCTCATTTAAGAAATTCCGTCAATTGAT
CCAAGTAAACCCAGATATCTTGATGCGCCTTTCAGGTCAAATGGCTCGTCGTCTGCAAGTGACCAGCCAGAAAGTTGGCG
ACCTTGCGTTCCTAGACGTAACTGGCCGTATCGCACAGACTCTGCTGAATCTTGCACGTCAGCCAGATGCGATGACTCAC
CCTGACGGTATGCAAATCAAGATCACTCGCCAAGAGATCGGTCAAATTGTTGGCTGTTCTCGCGAAACCGTTGGCCGCAT
CCTGAAGATGCTGGAAGAGCAGAACCTGATCTCTGCCCACGGTAAAACCATCGTGGTTTACGGCACTCGCTAA

Domains

Predicted by InterproScan.

Crp-like helix-turn-helix domain (HTH_Crp_2)

(142-206)

Cyclic nucleotide-binding domain (cNMP_binding)

(23-109)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G8D3R0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  crp Haemophilus influenzae Rd KW20

75.49

97.143

0.733
  crp Acinetobacter baumannii D1279779

47.343

98.571

0.467

Multiple sequence alignment    



References

[1] Melanie Blokesch (2012) Chitin colonization, chitin degradation and chitin-induced natural competence of Vibrio cholerae are subject to catabolite repression. Environmental Microbiology 14(8):1898-912. [PMID: 22222000]
[2] Mirella Lo Scrudato et al. (2012) The regulatory network of natural competence and transformation of Vibrio cholerae. PLoS Genetics 8(6):e1002778. [PMID: 22737089]