Detailed information NTG0001409

Experimentally validated

Overview

• Name: crp
• Type: Regulator
• Locus tag: HI_0957
• Genome accession: L42023
• Coordinates: 1014760..1015434 (+)
• Length: 224 a.a.
• NCBI ID: AAC22618.1
• Uniprot ID: -
• Organism: Haemophilus influenzae Rd KW20
• Function: promote expression of competence genes Competence regulation

Function

Interactions between cyclic AMP (cAMP) and its receptor protein CRP (cAMP receptor protein) are required but not sufficient to induce competence in H. influenzae.

Genomic Context

Location: 1009760..1020434

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HI_0950	-	1010675..1010845 (-)	171	AAC22611.1	ribosomal protein L33 (rpL33)	-
HI_0951	-	1010857..1011093 (-)	237	AAC22612.1	ribosomal protein L28 (rpL28)	-
HI_0952	-	1011307..1012011 (-)	705	AAC22613.1	DNA repair protein (radC)	-
HI_0953	-	1012136..1013338 (+)	1203	AAC22614.1	DNA/pantothenate metabolism flavoprotein (dfp)	-
HI_0954	-	1013386..1013841 (+)	456	AAC22615.1	deoxyuridinetriphosphatase (dut)	-
HI_0955	-	1013845..1014501 (+)	657	AAC22616.1	transcriptional regulator (ttk)	-
HI_0956	-	1014523..1014693 (+)	171	AAC22617.1	conserved hypothetical protein	-
HI_0957	crp	1014760..1015434 (+)	675	AAC22618.1	catabolite gene activator (crp)	Regulator
HI_0958	-	1015799..1016977 (-)	1179	AAC22619.1	RNA methyltransferase, putative	-
HI_0959	-	1016970..1018025 (-)	1056	AAC22620.1	beta-hexosaminidase (exoII)	-
HI_0960	-	1018029..1018379 (-)	351	AAC22628.1	predicted coding region HI0960	-
HI_0961	-	1018379..1018771 (-)	393	AAC22621.1	hit-related protein	-

Sequence

Protein

• Length: 224 a.a.
• Molecular weight: 25151.85 Da
• Isoelectric Point: 6.8375

>NTDB_id=1409 HI_0957 AAC22618.1 1014760..1015434(+) (crp) [Haemophilus influenzae Rd KW20]
MSNELTEIDEVVTSSQEEATQRDPVLDWFLTHCHLHKYPAKSTLIHAGEDATTLYYVIKGSVMVSSKDDEGKEMILTYLG
AGQFFGEAGLFDEGSKRSAWVKTKTTCEIAEISYKKYRQLIQANPEILMFLTAQLARRLQNTSRQVTNLAFLDVAGRIAQ
TLMNLAKQPEAMTHPDGMQIKITRQEIGQMVGCSRETVGRIIKMLEDQNLIHAHGKTIVVYGAR

Nucleotide

• Length: 675 bp

>NTDB_id=1409 HI_0957 AAC22618.1 1014760..1015434(+) (crp) [Haemophilus influenzae Rd KW20]
ATGTCAAATGAATTAACCGAAATTGATGAAGTTGTAACCTCCTCTCAAGAAGAAGCAACTCAACGAGATCCCGTTTTAGA
TTGGTTTCTTACTCACTGCCATTTGCATAAATATCCTGCAAAATCAACTTTAATTCATGCTGGGGAAGATGCGACCACGC
TGTATTATGTAATTAAAGGTTCTGTAATGGTATCTTCAAAAGATGATGAAGGCAAAGAGATGATCCTCACTTACTTAGGT
GCAGGACAATTTTTTGGCGAAGCGGGATTATTTGATGAAGGTTCAAAACGATCAGCTTGGGTAAAAACAAAAACAACATG
TGAAATTGCTGAAATTTCCTATAAGAAATATCGCCAGTTGATTCAGGCAAACCCTGAAATCTTAATGTTTCTCACTGCAC
AATTGGCAAGACGTTTGCAAAATACATCACGTCAAGTCACGAATTTGGCATTTTTAGACGTCGCAGGTCGCATCGCTCAA
ACTTTAATGAACTTAGCTAAACAGCCTGAAGCAATGACGCATCCTGATGGTATGCAAATCAAAATTACACGCCAAGAAAT
AGGGCAAATGGTGGGTTGTTCACGAGAAACTGTGGGGCGCATTATTAAGATGTTGGAGGATCAGAATCTTATCCACGCTC
ATGGAAAAACAATCGTTGTATATGGCGCAAGATAA

Domains

Predicted by InterproScan.

• Cyclic nucleotide-binding domain (cNMP_binding) (36-124)

• Crp-like helix-turn-helix domain (HTH_Crp_2) (156-220)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
crp	Vibrio cholerae strain A1552	75.49	97.143	0.733
crp	Acinetobacter baumannii D1279779	48.705	86.161	0.42

References

• [1]: Rosemary J Redfield et al. (2005) A novel CRP-dependent regulon controls expression of competence genes in Haemophilus influenzae. Journal of Molecular Biology 347(4):735-47. [PMID: 15769466]
• [2]: M S Chandler (1992) The gene encoding cAMP receptor protein is required for competence development in Haemophilus influenzae Rd. Proceedings of The National Academy of Sciences of The United States of America 89(5):1626-30. [PMID: 1542653]