Detailed information    

experimental Experimentally validated

Overview

Name   radC   Type   Machinery gene
Locus tag   HI_0952 Genome accession   L42023
Coordinates   1011307..1012011 (-) Length   234 a.a.
NCBI ID   AAC22613.1    Uniprot ID   -
Organism   Haemophilus influenzae Rd KW20     
Function   homologous recombination   
Homologous recombination

Function

radC is expressed specifically in cells competent for genetic transformation


Genomic Context

Location: 1006307..1017011
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HI_0946.1 - 1006630..1008165 (-) 1536 AAC22607.1 L-2,4-diaminobutyrate decarboxylase -
  HI_0947 - 1008185..1008583 (-) 399 AAC22608.1 virulence associated protein C (vapC) -
  HI_0948 - 1008583..1008816 (-) 234 AAC22609.1 conserved hypothetical protein -
  HI_0949 - 1008963..1010327 (-) 1365 AAC22610.1 aminotransferase -
  HI_0950 - 1010675..1010845 (-) 171 AAC22611.1 ribosomal protein L33 (rpL33) -
  HI_0951 - 1010857..1011093 (-) 237 AAC22612.1 ribosomal protein L28 (rpL28) -
  HI_0952 radC 1011307..1012011 (-) 705 AAC22613.1 DNA repair protein (radC) Machinery gene
  HI_0953 - 1012136..1013338 (+) 1203 AAC22614.1 DNA/pantothenate metabolism flavoprotein (dfp) -
  HI_0954 - 1013386..1013841 (+) 456 AAC22615.1 deoxyuridinetriphosphatase (dut) -
  HI_0955 - 1013845..1014501 (+) 657 AAC22616.1 transcriptional regulator (ttk) -
  HI_0956 - 1014523..1014693 (+) 171 AAC22617.1 conserved hypothetical protein -
  HI_0957 crp 1014760..1015434 (+) 675 AAC22618.1 catabolite gene activator (crp) Regulator
  HI_0958 - 1015799..1016977 (-) 1179 AAC22619.1 RNA methyltransferase, putative -

Sequence

Protein


Download         Length: 234 a.a.        Molecular weight: 26774.45 Da        Isoelectric Point: 7.3864

>NTDB_id=1480 HI_0952 AAC22613.1 1011307..1012011(-) (radC) [Haemophilus influenzae Rd KW20]
MRSKSFLFFFYKYMENNDELMPREKLLAFGAKALSDYELLAIFLRTGIKGCPVMSLSKNVLTHFGPLHALLSADKKAFCS
VKGLGITQFIQLQAITEMTKRYLKQDMLSTPIINDPETVKLFLLTELQHEEREVFMVLFLDNQHRLIKKERLFLGTIYVS
AVYPREIIKEALYCNAAALILAHNHPSGITEPSYSDQLITKKIQDAAELMEIRVLDHLIVGKSDCYSFAENCLL

Nucleotide


Download         Length: 705 bp        

>NTDB_id=1480 HI_0952 AAC22613.1 1011307..1012011(-) (radC) [Haemophilus influenzae Rd KW20]
GTGCGGTCAAAATCTTTCTTATTTTTCTTTTATAAATATATGGAAAACAATGATGAATTAATGCCACGGGAAAAATTGCT
GGCATTTGGAGCAAAAGCATTAAGTGATTATGAACTACTTGCGATTTTTTTACGTACTGGTATCAAAGGTTGTCCTGTTA
TGTCACTTTCTAAAAATGTATTAACGCATTTTGGCCCCTTGCACGCTTTACTTTCTGCAGATAAAAAAGCTTTCTGTTCT
GTAAAAGGCTTAGGTATTACGCAATTTATTCAACTGCAAGCGATTACGGAAATGACCAAGCGTTATTTAAAGCAAGATAT
GTTAAGTACGCCGATTATTAATGATCCTGAAACAGTAAAACTTTTTTTACTCACTGAATTGCAACACGAGGAAAGAGAAG
TATTTATGGTTTTATTTTTAGATAATCAACATCGTTTGATTAAAAAAGAGCGGTTATTTTTAGGTACAATTTATGTATCA
GCGGTCTATCCGCGAGAAATTATTAAAGAAGCACTCTATTGTAATGCGGCAGCTTTAATTTTGGCACATAATCATCCTTC
AGGTATAACGGAGCCGAGTTATTCGGATCAGCTTATTACTAAAAAAATTCAAGATGCGGCTGAACTAATGGAAATTCGCG
TGCTTGATCATTTAATTGTAGGGAAAAGCGATTGTTATTCTTTCGCGGAGAATTGTTTATTATAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(113-231)

UPF0758 N-terminal (UPF0758_N)

(20-87)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Sunita Sinha et al. (2012) Seventeen Sxy-dependent cyclic AMP receptor protein site-regulated genes are needed for natural transformation in Haemophilus influenzae. Journal of Bacteriology 194(19):5245-54. [PMID: 22821979]
[2] Rosemary J Redfield et al. (2005) A novel CRP-dependent regulon controls expression of competence genes in Haemophilus influenzae. Journal of Molecular Biology 347(4):735-47. [PMID: 15769466]