Detailed information NTG0001077

Experimentally validated

Overview

• Name: crp
• Type: Regulator
• Locus tag: ABD1_RS06150
• Genome accession: NC_020547
• Coordinates: 1284031..1284738 (-)
• Length: 235 a.a.
• NCBI ID: WP_000203218.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii D1279779
• Function: regulate competence development Competence regulation

Function

We examined the 33 knockout mutants by comparing their transformation efficiency with wild-type W068. No knockout strains were affected in growth, but 28 of these mutants were severely or completely impaired in natural transformability. These mutations were in 18 genes related to T4P (pilF, pilQ, tsaP, pilM, pilN, pilO, pilP, pilB, pilC, pilT, pilD, pilE, pilY2, pilY1, pilX, pilW, pilV, and fimU), six genes related to DNA uptake and processing (comEA, comA, comF, priA, dprA, and recA), two genes related to T2SS (xcpV and xcpW), and the crp and tonB2 genes.

Genomic Context

Location: 1279031..1289738

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABD1_RS06130 (ABD1_11890)	-	1280108..1281427 (+)	1320	WP_000517794.1	adenylosuccinate synthase	-
ABD1_RS06135	-	1281549..1281866 (+)	318	WP_000776215.1	hypothetical protein	-
ABD1_RS06140 (ABD1_11900)	-	1281983..1282762 (+)	780	WP_001034598.1	M48 family metallopeptidase	-
ABD1_RS06145 (ABD1_11910)	-	1282821..1283870 (-)	1050	WP_001159801.1	NADP(H)-dependent aldo-keto reductase	-
ABD1_RS06150 (ABD1_11920)	crp	1284031..1284738 (-)	708	WP_000203218.1	cAMP-activated global transcriptional regulator CRP	Regulator
ABD1_RS18320	-	1284981..1285100 (+)	120	Protein_1214	hypothetical protein	-
ABD1_RS06155 (ABD1_11940)	-	1285179..1285602 (+)	424	Protein_1215	OsmC family protein	-
ABD1_RS06160 (ABD1_11950)	-	1285696..1286121 (+)	426	WP_001026231.1	GNAT family N-acetyltransferase	-
ABD1_RS06165 (ABD1_11960)	-	1286118..1286924 (-)	807	WP_001237337.1	peptidoglycan DD-metalloendopeptidase family protein	-

Sequence

Protein

• Length: 235 a.a.
• Molecular weight: 26581.21 Da
• Isoelectric Point: 4.6676

>NTDB_id=1077 ABD1_RS06150 WP_000203218.1 1284031..1284738(-) (crp) [Acinetobacter baumannii D1279779]
MTSNFSQLSTDALSPGQLPESVKALLKRAHINRYPKRTTIVDAGTESKSLYLILKGSVSIILREDDEREIVVAYLNPGDF
FGEMGLFEPNPQRTAEVRTRDVCEIAEISYDNFHELSKQYPDLSYAVFAQLVRRLKNTTRKMTDLAFIDVSGRIARCLID
LSSQPEAMILPNGRQIRITRQEIGRIVGCSREMVGRVLKTLEDQGMIQTDGKAILIFDTSLEETPVTDEDYDEEE

Nucleotide

• Length: 708 bp

>NTDB_id=1077 ABD1_RS06150 WP_000203218.1 1284031..1284738(-) (crp) [Acinetobacter baumannii D1279779]
ATGACTTCAAATTTTTCACAACTCAGCACAGATGCTTTATCTCCGGGGCAACTACCTGAATCCGTTAAAGCATTGTTAAA
ACGTGCTCACATCAACAGATATCCAAAACGAACCACAATTGTTGATGCCGGAACAGAGTCAAAATCTTTATATTTAATTT
TAAAAGGCTCAGTTTCAATTATTTTACGTGAAGACGATGAACGTGAAATTGTTGTGGCATATTTGAATCCTGGTGACTTC
TTTGGGGAAATGGGGCTTTTCGAACCGAACCCTCAACGTACAGCTGAAGTTCGTACCCGTGATGTCTGTGAAATTGCGGA
AATTTCATATGACAACTTCCACGAACTGAGCAAACAGTATCCAGATCTCAGCTATGCCGTTTTCGCGCAACTCGTTCGTC
GTTTAAAAAATACAACTCGTAAAATGACCGATCTTGCATTTATTGATGTGTCGGGCCGTATTGCGCGTTGCTTAATCGAC
CTATCTTCACAACCAGAAGCAATGATCTTGCCGAATGGCCGTCAAATTCGTATTACTCGACAAGAGATTGGACGCATTGT
CGGGTGTTCACGAGAAATGGTTGGCCGTGTATTAAAGACCTTAGAAGATCAAGGTATGATTCAAACTGACGGTAAAGCTA
TTCTAATTTTTGATACTTCATTAGAAGAAACCCCAGTCACTGACGAAGACTATGATGAAGAAGAATAA

Domains

Predicted by InterproScan.

• Crp-like helix-turn-helix domain (HTH_Crp_2) (152-218)

• Cyclic nucleotide-binding domain (cNMP_binding) (33-120)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
crp	Vibrio cholerae strain A1552	47.343	98.571	0.467
crp	Haemophilus influenzae Rd KW20	48.705	86.161	0.42

References

• [1]: Yuan Hu et al. (2021) Natural Transformation in Acinetobacter baumannii W068: A Genetic Analysis Reveals the Involvements of the CRP, XcpV, XcpW, TsaP, and TonB₂. Frontiers in Microbiology 12:738034. [PMID: 35126321]