Detailed information    

experimental Experimentally validated

Overview

Name   comEA   Type   Machinery gene
Locus tag   A1552VC_RS08580 Genome accession   NZ_CP028894
Coordinates   1842560..1842871 (-) Length   103 a.a.
NCBI ID   WP_001166105.1    Uniprot ID   Q9KQT1
Organism   Vibrio cholerae strain A1552     
Function   dsDNA binding   
DNA binding and uptake

Function

ComEA shuttles the transforming DNA through the periplasm and toward the inner membrane aqueous channel ComEC, which, potentially in concert with ComF, transports a single DNA strand into the cytoplasm.


Genomic Context

Location: 1837560..1847871
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A1552VC_RS08555 (A1552VC_01689) lapB 1837754..1838923 (-) 1170 WP_000889971.1 lipopolysaccharide assembly protein LapB -
  A1552VC_RS08560 (A1552VC_01690) - 1838935..1839216 (-) 282 WP_000691492.1 LapA family protein -
  A1552VC_RS08565 (A1552VC_01691) ihfB 1839355..1839633 (-) 279 WP_000167341.1 integration host factor subunit beta -
  A1552VC_RS08570 (A1552VC_01692) rpsA 1839921..1841591 (-) 1671 WP_000140318.1 30S ribosomal protein S1 -
  A1552VC_RS08575 (A1552VC_01693) cmk 1841700..1842377 (-) 678 WP_000094752.1 (d)CMP kinase -
  A1552VC_RS08580 (A1552VC_01694) comEA 1842560..1842871 (-) 312 WP_001166105.1 ComEA family DNA-binding protein Machinery gene
  A1552VC_RS08585 (A1552VC_01695) ppiD 1843006..1844865 (-) 1860 WP_000969331.1 peptidylprolyl isomerase -
  A1552VC_RS08590 (A1552VC_01696) - 1845018..1845290 (-) 273 WP_001044516.1 HU family DNA-binding protein -
  A1552VC_RS08595 (A1552VC_01698) lon 1845474..1847834 (-) 2361 WP_001047611.1 endopeptidase La -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  tfoX comEA positive effect
  tfoX pilO positive effect
  tfoX pilB positive effect
  tfoX pilP positive effect
  tfoX pilA positive effect
  tfoX pilD positive effect
  tfoX dprA positive effect
  tfoX pilM positive effect
  tfoX pilN positive effect
  hapR comEA positive effect
  crp comEA positive effect
  qstR comEA positive effect
  tfoX pilQ positive effect
  tfoX qstR positive effect
  qstR comEC positive effect
  qstR comF positive effect
  qstR comM positive effect
  crp qstR positive effect
  hapR qstR positive effect
  tfoX comEC positive effect
  hapR comEC positive effect
  tfoX pilC positive effect
  chiS tfoX positive effect
  hapR dns negative effect

Sequence

Protein


Download         Length: 103 a.a.        Molecular weight: 10966.78 Da        Isoelectric Point: 5.0506

>NTDB_id=1150 A1552VC_RS08580 WP_001166105.1 1842560..1842871(-) (comEA) [Vibrio cholerae strain A1552]
MQIKTKIVTLFLSLCLPTLPLLANAEETAPAAQVEEGIVITVNINTASAEELATLLKGIGLKKAQAIVDYREANGPFTHI
DDLTNVKGIGEATVRNNAARILL

Nucleotide


Download         Length: 312 bp        

>NTDB_id=1150 A1552VC_RS08580 WP_001166105.1 1842560..1842871(-) (comEA) [Vibrio cholerae strain A1552]
ATGCAAATCAAAACCAAAATAGTGACACTGTTTCTTTCTCTCTGCCTGCCGACATTACCGTTACTGGCCAATGCCGAGGA
AACGGCACCCGCTGCGCAGGTAGAAGAAGGTATTGTGATCACTGTCAATATTAATACCGCTTCTGCAGAAGAGCTGGCGA
CGTTACTCAAAGGCATCGGGCTTAAGAAAGCTCAGGCCATTGTCGATTATCGAGAAGCCAACGGTCCTTTTACTCATATC
GATGATCTGACGAATGTGAAAGGGATTGGTGAAGCGACAGTGCGCAACAATGCCGCAAGGATCTTGTTATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(41-101)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9KQT1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Vibrio cholerae C6706

100

100

1
  comEA Vibrio parahaemolyticus RIMD 2210633

62.766

100

0.628
  comEA Vibrio campbellii strain DS40M4

58.333

100

0.589
  comE1/comEA Haemophilus influenzae Rd KW20

40.541

100

0.437
  Cj0011c Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

62.745

64.557

0.405

Multiple sequence alignment    



References

[1] Patrick Seitz et al. (2014) ComEA is essential for the transfer of external DNA into the periplasm in naturally transformable Vibrio cholerae cells. PLoS Genetics 10(1):e1004066. [PMID: 24391524]
[2] Patrick Seitz et al. (2014) DNA transport across the outer and inner membranes of naturally transformable Vibrio cholerae is spatially but not temporally coupled. MBio 5(4):e01409-14. [PMID: 25139903]
[3] Mirella Lo Scrudato et al. (2014) Regulatory elements involved in the expression of competence genes in naturally transformable Vibrio cholerae. BMC Microbiology 14:327. [PMID: 25539806]
[4] Patrick Seitz et al. (2013) DNA-uptake machinery of naturally competent Vibrio cholerae. Proceedings of The National Academy of Sciences of The United States of America 110(44):17987-92. [PMID: 24127573]
[5] Gaia Suckow et al. (2011) Quorum sensing contributes to natural transformation of Vibrio cholerae in a species-specific manner. Journal of Bacteriology 193(18):4914-24. [PMID: 21784943]