Detailed information    

experimental Experimentally validated

Overview

Name   comEA   Type   Machinery gene
Locus tag   ABDM36_RS04765 Genome accession   NZ_CP157384
Coordinates   1015642..1015953 (+) Length   103 a.a.
NCBI ID   WP_001166105.1    Uniprot ID   Q9KQT1
Organism   Vibrio cholerae C6706     
Function   dsDNA binding   
DNA binding and uptake

Function

ComEA shuttles the transforming DNA through the periplasm and toward the inner membrane aqueous channel ComEC, which, potentially in concert with ComF, transports a single DNA strand into the cytoplasm.


Genomic Context

Location: 1010642..1020953
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABDM36_RS04750 lon 1010679..1013039 (+) 2361 WP_001047611.1 endopeptidase La -
  ABDM36_RS04755 - 1013223..1013495 (+) 273 WP_001044516.1 HU family DNA-binding protein -
  ABDM36_RS04760 ppiD 1013648..1015507 (+) 1860 WP_000969331.1 peptidylprolyl isomerase -
  ABDM36_RS04765 comEA 1015642..1015953 (+) 312 WP_001166105.1 ComEA family DNA-binding protein Machinery gene
  ABDM36_RS04770 cmk 1016136..1016813 (+) 678 WP_000094752.1 (d)CMP kinase -
  ABDM36_RS04775 rpsA 1016922..1018592 (+) 1671 WP_000140318.1 30S ribosomal protein S1 -
  ABDM36_RS04780 ihfB 1018880..1019158 (+) 279 WP_000167341.1 integration host factor subunit beta -
  ABDM36_RS04785 - 1019297..1019578 (+) 282 WP_000691492.1 LapA family protein -
  ABDM36_RS04790 lapB 1019590..1020759 (+) 1170 WP_000889971.1 lipopolysaccharide assembly protein LapB -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  cytR comEA positive effect
  hapR comEA positive effect
  cytR pilA positive effect
  hapR pilA positive effect
  luxO hapR positive effect

Sequence

Protein


Download         Length: 103 a.a.        Molecular weight: 10966.78 Da        Isoelectric Point: 5.0506

>NTDB_id=1159 ABDM36_RS04765 WP_001166105.1 1015642..1015953(+) (comEA) [Vibrio cholerae C6706]
MQIKTKIVTLFLSLCLPTLPLLANAEETAPAAQVEEGIVITVNINTASAEELATLLKGIGLKKAQAIVDYREANGPFTHI
DDLTNVKGIGEATVRNNAARILL

Nucleotide


Download         Length: 312 bp        

>NTDB_id=1159 ABDM36_RS04765 WP_001166105.1 1015642..1015953(+) (comEA) [Vibrio cholerae C6706]
ATGCAAATCAAAACCAAAATAGTGACACTGTTTCTTTCTCTCTGCCTGCCGACATTACCGTTACTGGCCAATGCCGAGGA
AACGGCACCCGCTGCGCAGGTAGAAGAAGGTATTGTGATCACTGTCAATATTAATACCGCTTCTGCAGAAGAGCTGGCGA
CGTTACTCAAAGGCATCGGGCTTAAGAAAGCTCAGGCCATTGTCGATTATCGAGAAGCCAACGGTCCTTTTACTCATATC
GATGATCTGACGAATGTGAAAGGGATTGGTGAAGCGACAGTGCGCAACAATGCCGCAAGGATCTTGTTATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(41-101)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9KQT1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Vibrio cholerae strain A1552

100

100

1
  comEA Vibrio parahaemolyticus RIMD 2210633

62.766

100

0.628
  comEA Vibrio campbellii strain DS40M4

58.333

100

0.589
  comE1/comEA Haemophilus influenzae Rd KW20

40.541

100

0.437
  Cj0011c Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

62.745

64.557

0.405

Multiple sequence alignment    



References

[1] Elena S Antonova et al. (2012) Natural competence in Vibrio cholerae is controlled by a nucleoside scavenging response that requires CytR-dependent anti-activation. Molecular Microbiology 86(5):1215-31. [PMID: 23016895]