Detailed information    

experimental Experimentally validated

Overview

Name   cytR   Type   Regulator
Locus tag   ABDM36_RS01105 Genome accession   NZ_CP157384
Coordinates   237351..238358 (+) Length   335 a.a.
NCBI ID   WP_000224452.1    Uniprot ID   A0A085RG68
Organism   Vibrio cholerae C6706     
Function   promote competence gene expression   
Competence regulation

Function

A ΔcytR mutant was non-transformable and defective in expression of comEA and additional TfoX-induced genes, including pilA (transformation pseudopilus) and chiA-1 (chitinase).


Genomic Context

Location: 232351..243358
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABDM36_RS01085 metJ 232355..232672 (+) 318 WP_000796267.1 met regulon transcriptional regulator MetJ -
  ABDM36_RS01090 - 232783..234060 (-) 1278 WP_001278588.1 malic enzyme-like NAD(P)-binding protein -
  ABDM36_RS01095 rpmE 234368..234586 (-) 219 WP_000643446.1 50S ribosomal protein L31 -
  ABDM36_RS01100 priA 234841..237045 (+) 2205 WP_001247858.1 primosomal protein N' -
  ABDM36_RS01105 cytR 237351..238358 (+) 1008 WP_000224452.1 DNA-binding transcriptional regulator CytR Regulator
  ABDM36_RS01110 - 238530..239081 (+) 552 WP_001884022.1 SPOR domain-containing protein -
  ABDM36_RS01115 hslV 239218..239775 (+) 558 WP_000208249.1 ATP-dependent protease subunit HslV -
  ABDM36_RS01120 hslU 239841..241172 (+) 1332 WP_001293370.1 HslU--HslV peptidase ATPase subunit -
  ABDM36_RS01125 - 241276..242193 (+) 918 WP_001066162.1 1,4-dihydroxy-2-naphthoate polyprenyltransferase -
  ABDM36_RS01130 rraA 242268..242783 (+) 516 WP_000456236.1 ribonuclease E activity regulator RraA -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  cytR comEA positive effect
  cytR pilA positive effect
  hapR comEA positive effect
  hapR pilA positive effect
  luxO hapR positive effect

Sequence

Protein


Download         Length: 335 a.a.        Molecular weight: 37018.59 Da        Isoelectric Point: 7.0759

>NTDB_id=1158 ABDM36_RS01105 WP_000224452.1 237351..238358(+) (cytR) [Vibrio cholerae C6706]
MATMKDVAQLAGVSTATVSRALMNPEKVSSSTRKRVEEAVLEAGYSPNSLARNLRRNESKTIVAIVPDICDPYFSEIIRG
IEDAAMEHGYLVLLGDSGQQKRRENSFVNLVFTKQADGMLLLGTDLPFDVSKPEQKNLPPMVMACEFAPELELPTVHIDN
LTSAFEAVNYLTQLGHKRIAQISGPQHAALCQFRHQGYQQALRRAGITMNPTYCTFGDFTFEAGAKAVRQLLALPEQPTA
IFCHNDTMAIGAIQEAKRLGLRVPQDLSVVGFDDIQFAQYCDPPLTTISQPRYEIGRQAMLMMLELLRGHDVRAGSRLLE
TKLVVRESAAPPSKK

Nucleotide


Download         Length: 1008 bp        

>NTDB_id=1158 ABDM36_RS01105 WP_000224452.1 237351..238358(+) (cytR) [Vibrio cholerae C6706]
ATGGCGACAATGAAGGATGTTGCCCAGCTTGCTGGAGTTTCAACCGCGACCGTGTCTCGTGCGTTGATGAATCCAGAGAA
AGTCTCGTCGTCCACTCGTAAACGAGTGGAAGAGGCCGTGCTGGAAGCGGGTTACTCACCCAATTCTTTGGCGCGCAATT
TGCGCCGTAATGAATCGAAAACCATCGTTGCTATCGTGCCAGATATCTGTGATCCCTACTTTTCTGAGATCATTCGCGGT
ATTGAAGATGCCGCGATGGAGCACGGTTATCTGGTGCTGTTGGGGGATAGTGGTCAACAGAAGCGTCGGGAGAACTCGTT
CGTTAATCTGGTGTTTACCAAACAGGCAGACGGTATGTTGCTGCTGGGGACAGATTTGCCGTTTGATGTGAGTAAACCTG
AGCAGAAAAACCTACCGCCTATGGTGATGGCGTGTGAATTTGCGCCAGAGCTGGAATTGCCAACGGTACACATTGATAAC
CTGACCTCGGCATTTGAGGCGGTAAACTATCTCACTCAGCTTGGGCACAAACGGATTGCGCAGATCTCAGGTCCACAACA
TGCTGCGCTGTGCCAATTCCGTCATCAAGGCTATCAACAGGCGTTGCGTCGTGCTGGCATCACCATGAACCCGACTTACT
GCACTTTTGGCGATTTCACTTTTGAAGCCGGGGCAAAAGCGGTGCGTCAGCTGTTGGCATTGCCTGAGCAGCCGACCGCG
ATTTTCTGTCATAACGATACGATGGCGATTGGCGCAATTCAGGAAGCGAAGCGACTTGGCTTGCGCGTACCGCAAGATCT
CTCTGTGGTCGGTTTTGACGATATTCAGTTTGCCCAGTACTGCGATCCCCCATTAACCACCATTTCGCAGCCGCGTTATG
AGATTGGCCGTCAAGCCATGCTGATGATGCTTGAGCTGCTTCGCGGACACGATGTACGTGCTGGATCGCGTCTTTTGGAG
ACCAAGCTGGTCGTGCGTGAGAGTGCTGCGCCGCCAAGCAAGAAGTAA

Domains

Predicted by InterproScan.

Bacterial regulatory proteins, lacI family (LacI)

(3-48)

Periplasmic binding protein-like domain (Peripla_BP_3)

(168-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A085RG68

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  cytR Vibrio parahaemolyticus RIMD 2210633

90.719

99.701

0.904

Multiple sequence alignment    



References

[1] Samit S Watve et al. (2015) CytR Is a Global Positive Regulator of Competence, Type VI Secretion, and Chitinases in Vibrio cholerae. PloS One 10(9):e0138834. [PMID: 26401962]
[2] Elena S Antonova et al. (2012) Natural competence in Vibrio cholerae is controlled by a nucleoside scavenging response that requires CytR-dependent anti-activation. Molecular Microbiology 86(5):1215-31. [PMID: 23016895]