Detailed information    

experimental Experimentally validated

Overview

Name   hapR   Type   Regulator
Locus tag   ABDM36_RS11380 Genome accession   NZ_CP157384
Coordinates   2522280..2522891 (+) Length   203 a.a.
NCBI ID   WP_000340098.1    Uniprot ID   B2CKP3
Organism   Vibrio cholerae C6706     
Function   regulate expression of competence genes   
Competence regulation

Function

Expression of all three genes in a ΔhapR and a ΔcytR mutant was approximately as low as in the strains lacking constitutive TfoX, which suggested that not only comEA, but also chiA-1, and pilA are positively regulated by both HapR and CytR.


Genomic Context

Location: 2517280..2527891
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABDM36_RS11360 - 2517613..2518530 (-) 918 WP_000271063.1 ABC transporter ATP-binding protein -
  ABDM36_RS11365 - 2518914..2520560 (-) 1647 WP_000484911.1 SulP family inorganic anion transporter -
  ABDM36_RS11370 can 2520708..2521376 (+) 669 WP_001114117.1 carbonate dehydratase -
  ABDM36_RS11375 hpt 2521451..2521984 (-) 534 WP_000683351.1 hypoxanthine phosphoribosyltransferase -
  ABDM36_RS11380 hapR 2522280..2522891 (+) 612 WP_000340098.1 quorum-sensing master transcriptional regulator HapR Regulator
  ABDM36_RS11385 rsmI 2523105..2523971 (-) 867 WP_000131542.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  ABDM36_RS11390 - 2524041..2525846 (+) 1806 WP_001015481.1 penicillin-binding protein activator -
  ABDM36_RS11395 - 2525833..2526201 (+) 369 WP_001893625.1 YraN family protein -
  ABDM36_RS11400 - 2526205..2526795 (+) 591 WP_000887295.1 phosphoheptose isomerase -
  ABDM36_RS11405 - 2526798..2527367 (+) 570 WP_000836326.1 BON domain-containing protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  hapR comEA positive effect
  cytR comEA positive effect
  cytR pilA positive effect
  hapR pilA positive effect
  luxO hapR positive effect

Sequence

Protein


Download         Length: 203 a.a.        Molecular weight: 23692.39 Da        Isoelectric Point: 6.6356

>NTDB_id=1161 ABDM36_RS11380 WP_000340098.1 2522280..2522891(+) (hapR) [Vibrio cholerae C6706]
MDASIEKRPRTRLSPQKRKLQLMEIALEVFAKRGIGRGGHADIAEIAQVSVATVFNYFPTREDLVDDVLNFVVRQYSNFL
TDHIDLDLDVKTNLQTLCKEMVKLAMTDCHWLKVWFEWSASTRDEVWPLFVSTNRTNQLLIRNMFMKAMERGELCEKHDV
DNMASLFHGIFYSIFLQVNRLGEQEAVYKLADSYLNMLCIYKN

Nucleotide


Download         Length: 612 bp        

>NTDB_id=1161 ABDM36_RS11380 WP_000340098.1 2522280..2522891(+) (hapR) [Vibrio cholerae C6706]
ATGGACGCATCAATCGAAAAACGCCCTCGAACTCGGCTATCGCCTCAAAAACGCAAACTACAACTGATGGAAATCGCGTT
GGAAGTGTTTGCTAAACGCGGCATTGGTCGTGGTGGTCACGCAGATATTGCCGAGATTGCGCAAGTCTCCGTTGCAACAG
TGTTCAACTACTTCCCAACTCGTGAAGACTTGGTTGACGATGTGCTGAATTTTGTGGTTCGTCAGTACTCCAACTTCTTG
ACCGATCACATCGATCTTGATTTGGATGTGAAAACCAACCTACAAACTCTGTGCAAAGAGATGGTGAAATTGGCGATGAC
CGATTGTCACTGGCTCAAAGTCTGGTTTGAGTGGAGTGCTTCAACCCGTGACGAAGTTTGGCCACTGTTTGTTTCCACCA
ACCGAACTAACCAACTGCTGATCAGAAACATGTTTATGAAAGCGATGGAGCGTGGCGAATTGTGTGAGAAACACGATGTC
GATAACATGGCCAGCCTGTTCCACGGCATCTTCTACTCCATCTTCTTACAAGTGAACCGTTTAGGTGAACAAGAAGCAGT
GTATAAGTTGGCCGATAGCTACCTCAATATGCTGTGTATCTATAAGAACTAG

Domains

Predicted by InterproScan.

Bacterial regulatory proteins, tetR family (TetR_N)

(23-67)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 6D7R
  PDB 7XXN
  PDB 7XXO
  PDB 7XXS
  PDB 7XXT
  PDB 7XY0
  PDB 7XY5
  PDB 7XYI
  PDB 7Y4J

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  hapR Vibrio cholerae strain A1552

100

100

1
  opaR Vibrio parahaemolyticus RIMD 2210633

72.222

97.537

0.704

Multiple sequence alignment    



References

[1] Mirella Lo Scrudato et al. (2013) A transcriptional regulator linking quorum sensing and chitin induction to render Vibrio cholerae naturally transformable. Nucleic Acids Research 41(6):3644-58. [PMID: 23382174]
[2] Mirella Lo Scrudato et al. (2012) The regulatory network of natural competence and transformation of Vibrio cholerae. PLoS Genetics 8(6):e1002778. [PMID: 22737089]
[3] Elena S Antonova et al. (2012) Natural competence in Vibrio cholerae is controlled by a nucleoside scavenging response that requires CytR-dependent anti-activation. Molecular Microbiology 86(5):1215-31. [PMID: 23016895]
[4] Gaia Suckow et al. (2011) Quorum sensing contributes to natural transformation of Vibrio cholerae in a species-specific manner. Journal of Bacteriology 193(18):4914-24. [PMID: 21784943]
[5] Karin L Meibom et al. (2005) Chitin induces natural competence in Vibrio cholerae. Science (New York, N.Y.) 310(5755):1824-7. [PMID: 16357262]