Detailed information    

experimental Experimentally validated

Overview

Name   luxO   Type   Regulator
Locus tag   A1552VC_RS03895 Genome accession   NZ_CP028894
Coordinates   818142..819509 (+) Length   455 a.a.
NCBI ID   WP_001888250.1    Uniprot ID   Q9KT84
Organism   Vibrio cholerae strain A1552     
Function   promote HapR production   
Competence regulation

Function

Expression of all three genes in a ΔhapR and a ΔcytR mutant was approximately as low as in the strains lacking constitutive TfoX, which suggested that not only comEA, but also chiA-1, and pilA are positively regulated by both HapR and CytR.


Genomic Context

Location: 813142..824509
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A1552VC_RS03870 (A1552VC_00764) rsxB 813631..814218 (-) 588 WP_000104488.1 electron transport complex subunit RsxB -
  A1552VC_RS03875 (A1552VC_00765) rsxA 814218..814799 (-) 582 WP_000141550.1 electron transport complex subunit RsxA -
  A1552VC_RS03885 (A1552VC_00767) uvrB 815681..817711 (+) 2031 WP_000029983.1 excinuclease ABC subunit UvrB -
  A1552VC_RS03895 (A1552VC_00769) luxO 818142..819509 (+) 1368 WP_001888250.1 quorum-sensing sigma-54 dependent transcriptional regulator LuxO Regulator
  A1552VC_RS03900 (A1552VC_00770) luxU 819513..819854 (+) 342 WP_001209531.1 quorum-sensing phosphorelay protein LuxU -
  A1552VC_RS03905 (A1552VC_00771) - 819843..820733 (-) 891 WP_000061070.1 YvcK family protein -
  A1552VC_RS03910 (A1552VC_00773) moaA 821039..822028 (+) 990 WP_000068572.1 GTP 3',8-cyclase MoaA -
  A1552VC_RS03915 (A1552VC_00774) moaB 822088..822600 (+) 513 WP_000509705.1 molybdenum cofactor biosynthesis protein B -
  A1552VC_RS03920 (A1552VC_00775) moaC 822611..823090 (+) 480 WP_000080915.1 cyclic pyranopterin monophosphate synthase MoaC -
  A1552VC_RS03925 (A1552VC_00776) moaD 823087..823332 (+) 246 WP_000598590.1 molybdopterin synthase sulfur carrier subunit -
  A1552VC_RS03930 (A1552VC_00777) moaE 823334..823786 (+) 453 WP_000350081.1 molybdopterin synthase catalytic subunit MoaE -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  luxO hapR positive effect
  cqsS luxO positive effect
  luxQ luxO positive effect
  luxP luxO positive effect
  cqsA cqsS positive effect
  luxS luxQ positive effect
  luxS luxP positive effect

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 50839.12 Da        Isoelectric Point: 5.5755

>NTDB_id=1157 A1552VC_RS03895 WP_001888250.1 818142..819509(+) (luxO) [Vibrio cholerae strain A1552]
MVEDTASVAALYRSYLTPLDIDINIVGTGRDAIESIGRREPDLILLDLRLPDMTGMDVLYAVKEKSPDVPIVFMTAHGSI
DTAVEAMRHGAQDFLIKPCEADRLRVTVNNAIRKASKLKNDVDNKNQNYQGFIGSSQTMQAVYRTIDSAASSKASIFITG
ESGTGKEVCAEAIHAASKRGDKPFIAINCAAIPKDLIESELFGHVKGAFTGAATERQGAAEAADGGTLFLDELCEMDLDL
QTKLLRFIQTGTFQKVGSSKMKSVDVRFVCATNRDPWKEVQEGRFREDLYYRLYVIPLHLPPLRARGDDVIEIAYSLLGF
MSKEEGKDFVRLSAEVVERFRQYEWPGNVRQLQNVLRNVVVLNEGREITLDMLPPPLNQMSAPINRALPLAHENKVSVHE
IFPLWMTEKQAIEQAIEACDGNIPRAATYLDVSPSTIYRKLQTWNEKVKEKEKER

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=1157 A1552VC_RS03895 WP_001888250.1 818142..819509(+) (luxO) [Vibrio cholerae strain A1552]
ATGGTAGAAGACACGGCGTCGGTGGCGGCGCTGTATCGTTCTTACCTCACACCGCTGGATATTGATATCAATATCGTGGG
TACCGGACGCGATGCCATCGAGAGTATTGGCCGTCGCGAGCCTGACTTAATCCTGCTGGATTTACGTTTGCCAGATATGA
CGGGGATGGACGTACTCTATGCAGTGAAAGAGAAATCACCGGATGTGCCTATCGTGTTTATGACCGCTCATGGTTCGATT
GATACGGCGGTTGAAGCCATGCGTCATGGTGCGCAAGACTTTTTGATCAAGCCGTGTGAAGCGGACCGACTGCGGGTCAC
AGTGAATAATGCGATTCGCAAAGCCTCGAAACTCAAAAACGATGTTGATAATAAAAATCAAAACTATCAAGGCTTTATTG
GTAGCAGTCAAACCATGCAGGCGGTGTACCGCACCATTGACTCTGCGGCGAGCAGCAAAGCCAGTATTTTTATTACCGGC
GAAAGTGGTACAGGTAAAGAAGTGTGCGCGGAAGCGATTCATGCCGCGAGCAAGCGTGGAGATAAGCCATTTATCGCCAT
CAACTGTGCGGCCATTCCGAAAGATCTGATTGAAAGTGAGTTGTTTGGTCACGTCAAAGGGGCTTTTACTGGGGCGGCGA
CTGAGCGTCAAGGCGCGGCAGAAGCGGCTGATGGGGGAACCCTCTTTTTGGATGAATTGTGCGAAATGGATCTGGATCTG
CAGACCAAACTTCTGCGCTTTATTCAGACAGGGACATTCCAAAAAGTCGGCTCTTCCAAAATGAAAAGCGTGGATGTGCG
TTTTGTGTGTGCAACGAACCGCGATCCGTGGAAGGAAGTGCAAGAAGGACGCTTTCGTGAAGACCTGTACTACCGCTTGT
ATGTGATCCCGCTGCATTTACCGCCATTGCGCGCGCGTGGTGATGATGTGATCGAGATTGCTTATTCACTGCTTGGCTTT
ATGTCCAAGGAAGAGGGCAAAGATTTTGTCCGTTTGTCGGCTGAAGTGGTGGAGCGTTTTCGCCAATACGAGTGGCCGGG
CAACGTGCGTCAATTGCAAAACGTTTTGCGCAACGTGGTTGTGCTCAATGAAGGTCGTGAAATCACCCTAGATATGCTGC
CTCCTCCTCTTAATCAAATGTCCGCGCCGATCAATCGGGCTTTACCGCTTGCGCATGAGAATAAAGTATCCGTGCATGAG
ATTTTTCCGCTGTGGATGACCGAAAAACAAGCCATTGAACAAGCCATCGAAGCGTGTGATGGCAACATTCCCCGTGCCGC
AACCTATCTGGATGTCAGCCCGTCAACCATCTATCGCAAGCTGCAAACTTGGAATGAAAAAGTGAAAGAAAAAGAGAAGG
AACGGTAA

Domains

Predicted by InterproScan.

Bacterial regulatory protein, Fis family (HTH_8)

(404-442)

Sigma-54 interaction domain (Sigma54_activat)

(133-298)

Response regulator receiver domain (Response_reg)

(2-108)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9KT84

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

38.17

100

0.384

Multiple sequence alignment    



References

[1] Calum Johnston et al. (2014) Bacterial transformation: distribution, shared mechanisms and divergent control. Nature Reviews. Microbiology 12(3):181-96. [PMID: 24509783]
[2] Gaia Suckow et al. (2011) Quorum sensing contributes to natural transformation of Vibrio cholerae in a species-specific manner. Journal of Bacteriology 193(18):4914-24. [PMID: 21784943]