Detailed information    

experimental Experimentally validated

Overview

Name   pilR   Type   Regulator
Locus tag   PAKAF_RS24000 Genome accession   NZ_LR657304
Coordinates   5203736..5205073 (+) Length   445 a.a.
NCBI ID   WP_003094694.1    Uniprot ID   A0A0H2ZG94
Organism   Pseudomonas aeruginosa PAK     
Function   regulate pilin expression   
Competence regulation

Function

PilR contains the amino-terminal aspartic acid residues which are conserved among the response regulators, suggesting that pilin gene transcription is regulated via a phosphotransfer mechanism in which PilR is phosphorylated by an as yet unidentified protein kinase. PilS is predicted to be a sensor protein which when stimulated by the appropriate environmental signals activates PilR through kinase activity. PilR then activates transcription of pilA, probably by interacting with RNA polymerase containing RpoN.


Genomic Context

Location: 5198736..5210073
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PAKAF_RS23975 (PAKAF_04817) pgeF 5198879..5199607 (-) 729 WP_016253892.1 peptidoglycan editing factor PgeF -
  PAKAF_RS23980 (PAKAF_04818) rluD 5199604..5200566 (-) 963 WP_003094686.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  PAKAF_RS23985 (PAKAF_04819) - 5200712..5201737 (+) 1026 WP_003102590.1 outer membrane protein assembly factor BamD -
  PAKAF_RS23990 (PAKAF_04820) - 5201894..5202139 (+) 246 WP_009316269.1 PP0621 family protein -
  PAKAF_RS23995 (PAKAF_04821) pilS 5202129..5203721 (+) 1593 WP_016253893.1 two-component system sensor histidine kinase PilS Regulator
  PAKAF_RS24000 (PAKAF_04822) pilR 5203736..5205073 (+) 1338 WP_003094694.1 two-component system response regulator PilR Regulator
  PAKAF_RS24005 thiO 5205119..5206213 (-) 1095 WP_016253894.1 glycine oxidase ThiO -
  PAKAF_RS24010 (PAKAF_04824) - 5206348..5206857 (+) 510 WP_003161431.1 Tfp pilus assembly protein FimT/FimU -
  PAKAF_RS24015 (PAKAF_04825) - 5206963..5207469 (+) 507 WP_003102598.1 Tfp pilus assembly protein FimT/FimU -
  PAKAF_RS24020 (PAKAF_04826) pilV 5207460..5208017 (+) 558 WP_016253895.1 type 4a pilus minor pilin PilV Machinery gene
  PAKAF_RS24025 (PAKAF_04827) pilW 5208014..5208838 (+) 825 WP_016253896.1 type 4a pilus minor pilin PilW -
  PAKAF_RS24030 (PAKAF_04828) pilX 5208835..5209422 (+) 588 WP_015648054.1 type 4a pilus minor pilin PilX -

Sequence

Protein


Download         Length: 445 a.a.        Molecular weight: 49737.06 Da        Isoelectric Point: 5.8654

>NTDB_id=1473 PAKAF_RS24000 WP_003094694.1 5203736..5205073(+) (pilR) [Pseudomonas aeruginosa PAK]
MSRQKALIVDDEPDIRELLEITLGRMKLDTRSARNVKEARELLAREPFDLCLTDMRLPDGSGLDLVQYIQQRHPQTPVAM
ITAYGSLDTAIQALKAGAFDFLTKPVDLGRLRELVATALRLRNPEAEEAPVDNRLLGESPPMRALRNQIGKLARSQAPVY
ISGESGSGKELVARLIHEQGPRIERPFVPVNCGAIPSELMESEFFGHKKGSFTGAIEDKQGLFQAASGGTLFLDEVADLP
MAMQVKLLRAIQEKAVRAVGGQQEVAVDVRILCATHKDLAAEVGAGRFRQDLYYRLNVIELRVPPLRERREDIPLLAERI
LKRLAGDTGLPAARLTGDAQEKLKNYRFPGNVRELENMLERAYTLCEDDQIQPHDLRLADAPGASQEGAASLSEIDNLED
YLEDIERKLIMQALEETRWNRTAAAQRLGLTFRSMRYRLKKLGID

Nucleotide


Download         Length: 1338 bp        

>NTDB_id=1473 PAKAF_RS24000 WP_003094694.1 5203736..5205073(+) (pilR) [Pseudomonas aeruginosa PAK]
ATGAGCCGACAAAAAGCCCTGATCGTCGACGATGAACCGGATATCCGCGAACTGCTGGAAATCACTCTCGGCCGCATGAA
GCTGGACACCCGCAGCGCCCGCAACGTCAAGGAAGCCCGCGAGTTGCTGGCCCGCGAGCCGTTCGACCTGTGCCTCACCG
ACATGCGCCTGCCGGACGGCAGCGGCCTCGATCTGGTCCAGTACATCCAGCAGCGCCATCCACAGACCCCGGTGGCCATG
ATCACCGCGTACGGCAGCCTGGACACCGCGATCCAGGCGCTCAAGGCCGGTGCCTTCGACTTCCTCACCAAACCGGTCGA
CCTCGGTCGCTTGCGGGAGCTGGTGGCAACCGCCCTGCGCTTGCGCAACCCGGAAGCCGAGGAAGCGCCGGTGGACAACC
GCCTGCTCGGCGAGTCGCCGCCGATGCGCGCCCTGCGCAACCAGATCGGCAAGCTGGCGCGCAGCCAGGCGCCGGTCTAC
ATCAGTGGCGAGTCCGGTAGCGGCAAGGAGCTGGTGGCGCGCCTGATCCACGAGCAGGGGCCACGTATCGAGCGGCCGTT
CGTGCCGGTGAACTGCGGCGCGATTCCCTCCGAACTGATGGAAAGCGAGTTCTTCGGCCACAAGAAAGGCAGCTTCACTG
GCGCTATCGAAGACAAGCAGGGCCTGTTCCAGGCCGCCAGCGGCGGCACCCTGTTCCTCGACGAAGTCGCCGACCTGCCG
ATGGCCATGCAGGTCAAACTGCTCCGGGCGATCCAGGAAAAGGCCGTGCGCGCGGTCGGCGGCCAGCAGGAGGTCGCCGT
CGACGTGCGCATCCTCTGCGCCACCCACAAGGACCTCGCCGCCGAAGTCGGCGCCGGGCGCTTCCGCCAGGACCTCTACT
ACCGCCTCAACGTCATCGAGCTGCGCGTACCGCCGCTGCGCGAACGCCGCGAGGACATCCCGCTGCTCGCCGAACGCATC
CTCAAGCGCCTGGCCGGCGACACCGGCCTGCCGGCCGCCAGGCTGACCGGCGACGCACAGGAGAAGCTGAAGAACTACCG
CTTCCCGGGCAACGTCCGCGAGCTGGAAAACATGCTGGAGCGCGCCTACACCCTGTGCGAAGACGACCAGATCCAGCCTC
ACGACCTGCGCCTGGCCGATGCGCCGGGTGCCAGCCAGGAAGGCGCCGCGAGCCTGAGCGAAATAGACAACCTCGAGGAC
TACCTGGAAGACATCGAGCGCAAGCTGATCATGCAGGCGCTCGAGGAGACCCGCTGGAACCGCACCGCCGCGGCCCAGCG
CCTGGGCCTGACGTTCCGCTCGATGCGCTACCGCCTGAAAAAGCTGGGCATCGACTGA

Domains

Predicted by InterproScan.

Response regulator receiver domain (Response_reg)

(7-115)

Sigma-54 interaction domain (Sigma54_activat)

(135-301)

Bacterial regulatory protein, Fis family (HTH_8)

(402-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZG94

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Acinetobacter baumannii strain A118

51.198

100

0.528
  luxO Vibrio cholerae strain A1552

38.17

100

0.384

Multiple sequence alignment    



References

[1] J M Boyd et al. (1994) Identification and characterization of PilS, an essential regulator of pilin expression in Pseudomonas aeruginosa. Molecular & General Genetics : MGG 243(5):565-74. [PMID: 7911557]
[2] M Hobbs et al. (1993) PilS and PilR, a two-component transcriptional regulatory system controlling expression of type 4 fimbriae in Pseudomonas aeruginosa. Molecular Microbiology 7(5):669-82. [PMID: 8097014]
[3] K S Ishimoto et al. (1992) Identification of pilR, which encodes a transcriptional activator of the Pseudomonas aeruginosa pilin gene. Journal of Bacteriology 174(11):3514-21. [PMID: 1317379]