Detailed information NTG0001472

Experimentally validated

Overview

• Name: pilS
• Type: Regulator
• Locus tag: PAKAF_RS23995
• Genome accession: NZ_LR657304
• Coordinates: 5202129..5203721 (+)
• Length: 530 a.a.
• NCBI ID: WP_016253893.1
• Uniprot ID: Q51530
• Organism: Pseudomonas aeruginosa PAK
• Function: regulate pilin expression Competence regulation

Function

Deletion analysis of the pilS promoter fused to a promoterless lacZ gene further showed that a significant region upstream of pilS is essential for expression of pilS and pilR, suggesting a need for transcriptional activation. The pilA promoter can be activated in E. coli but only when PilR and sigma 54 are present. This work suggests that the PilS activation signal is received in the bacterial cytoplasm, and that the mechanism of PilS/PilR-mediated signal transduction resulting in activation of the pilin gene promoter is likely to be similar to that of other two-component systems. PilS is predicted to be a sensor protein which when stimulated by the appropriate environmental signals activates PilR through kinase activity. PilR then activates transcription of pilA, probably by interacting with RNA polymerase containing RpoN.

Genomic Context

Location: 5197129..5208721

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PAKAF_RS23975 (PAKAF_04817)	pgeF	5198879..5199607 (-)	729	WP_016253892.1	peptidoglycan editing factor PgeF	-
PAKAF_RS23980 (PAKAF_04818)	rluD	5199604..5200566 (-)	963	WP_003094686.1	23S rRNA pseudouridine(1911/1915/1917) synthase RluD	-
PAKAF_RS23985 (PAKAF_04819)	-	5200712..5201737 (+)	1026	WP_003102590.1	outer membrane protein assembly factor BamD	-
PAKAF_RS23990 (PAKAF_04820)	-	5201894..5202139 (+)	246	WP_009316269.1	PP0621 family protein	-
PAKAF_RS23995 (PAKAF_04821)	pilS	5202129..5203721 (+)	1593	WP_016253893.1	two-component system sensor histidine kinase PilS	Regulator
PAKAF_RS24000 (PAKAF_04822)	pilR	5203736..5205073 (+)	1338	WP_003094694.1	two-component system response regulator PilR	Regulator
PAKAF_RS24005	thiO	5205119..5206213 (-)	1095	WP_016253894.1	glycine oxidase ThiO	-
PAKAF_RS24010 (PAKAF_04824)	-	5206348..5206857 (+)	510	WP_003161431.1	Tfp pilus assembly protein FimT/FimU	-
PAKAF_RS24015 (PAKAF_04825)	-	5206963..5207469 (+)	507	WP_003102598.1	Tfp pilus assembly protein FimT/FimU	-
PAKAF_RS24020 (PAKAF_04826)	pilV	5207460..5208017 (+)	558	WP_016253895.1	type 4a pilus minor pilin PilV	Machinery gene

Sequence

Protein

• Length: 530 a.a.
• Molecular weight: 59000.17 Da
• Isoelectric Point: 6.5650

>NTDB_id=1472 PAKAF_RS23995 WP_016253893.1 5202129..5203721(+) (pilS) [Pseudomonas aeruginosa PAK]
MRAERLRLSEEQGQRILRLYHLYRLTIGLVLVLLISSELEDQVLKLVHPELFHVGSWCYLVFNILVALFLPPSRQLLPIF
ILALTDVLMLCGLFYAGGGVPSGIGSLLVVAVAIANILLRGRIGLVIAAAASLGLLYLTFFLSLSSPDATNHYVQAGGLG
TLCFAAALVIQALVRRQEQTETLAEERAETVANLEELNALILQRMRTGILVVDSRQAILLANQAALGLLRQDDVQGASLG
RHSPMLMHCMKQWRLNPSLRPPTLKVVPDGPTVQPSFISLNREDDQHVLIFLEDISQIAQQAQQMKLAGLGRLTAGIAHE
IRNPLGAISHAAQLLQESEELDAPDRRLTQIIQDQSKRMNLVIENVLQLSRRRQAEPQQLDLKEWLQRFVDEYPGRLRND
SELHLQLGAGDIQTRMDPHQLNQVLSNLVQNGLRYSAQAHGRGQVWLSLARDPESDLPVLEIIDDGPGVPADKLNNLFEP
FFTTESKGTGLGLYLSRELCESNQARIDYRNREEGGGCFRITFAHPRKLS

Nucleotide

• Length: 1593 bp

>NTDB_id=1472 PAKAF_RS23995 WP_016253893.1 5202129..5203721(+) (pilS) [Pseudomonas aeruginosa PAK]
GTGCGCGCTGAACGGCTACGGCTGAGCGAGGAGCAGGGGCAACGCATCCTCCGTCTGTACCACCTGTACCGCCTGACCAT
CGGCCTGGTACTGGTCCTGCTGATCTCCAGCGAACTGGAAGATCAGGTCCTCAAGCTCGTCCACCCTGAACTGTTCCATG
TCGGCAGTTGGTGCTACCTGGTCTTCAACATCCTGGTCGCGCTGTTCCTGCCACCGTCGCGGCAATTGCTGCCGATCTTC
ATCCTCGCGCTCACCGACGTGCTGATGCTTTGCGGCCTGTTCTACGCAGGTGGCGGCGTACCCAGCGGCATCGGCAGCCT
GCTGGTGGTGGCGGTGGCCATTGCCAACATCCTGCTGCGCGGGCGCATCGGCCTGGTCATCGCGGCGGCGGCCAGCCTCG
GCCTGCTCTACCTGACCTTCTTCCTCAGCCTGAGCAGTCCGGACGCCACCAACCACTACGTCCAGGCCGGCGGCCTCGGC
ACCCTGTGCTTCGCCGCCGCGCTGGTGATCCAGGCTCTGGTGCGGCGCCAGGAGCAGACCGAAACGCTGGCCGAAGAACG
CGCCGAGACGGTCGCCAACCTGGAGGAACTCAACGCATTGATCCTGCAGCGCATGCGCACCGGCATCCTCGTGGTCGATA
GCCGTCAGGCCATCCTCCTCGCCAACCAGGCCGCCCTCGGCCTGCTCAGGCAGGACGACGTGCAGGGCGCCAGCCTCGGC
CGCCACAGCCCGATGCTGATGCACTGCATGAAGCAATGGCGCCTGAATCCCAGCCTCCGTCCGCCGACGCTCAAGGTGGT
GCCGGACGGCCCGACGGTGCAACCCAGCTTTATCAGCCTCAACCGCGAAGACGACCAGCACGTGCTGATCTTCCTCGAAG
ACATTTCGCAGATCGCCCAGCAGGCGCAGCAGATGAAGCTGGCCGGTCTCGGCCGCCTGACCGCCGGCATCGCCCATGAG
ATCCGCAACCCGCTGGGCGCGATCAGCCACGCCGCCCAACTGCTGCAGGAGTCAGAGGAACTGGATGCCCCGGACCGACG
GCTGACGCAGATCATCCAGGACCAGTCGAAGCGGATGAACCTGGTCATCGAGAACGTCCTGCAGCTCTCCCGTCGCCGCC
AGGCCGAACCGCAGCAGCTCGACCTGAAGGAGTGGCTTCAGCGGTTCGTCGACGAATACCCCGGCAGGCTGCGCAACGAC
AGCGAACTGCACCTGCAGCTCGGTGCCGGCGACATCCAGACCCGCATGGACCCACACCAGTTGAACCAGGTGCTGAGCAA
CCTGGTGCAGAACGGTCTTCGCTACAGCGCCCAGGCGCACGGGCGCGGCCAGGTCTGGCTGAGCCTCGCGCGCGACCCGG
AGAGCGACCTGCCGGTGCTGGAAATCATCGACGACGGTCCCGGCGTACCGGCGGACAAACTGAACAACCTGTTCGAACCC
TTCTTTACTACAGAAAGCAAAGGCACCGGCCTGGGCCTCTATCTCTCCCGCGAGCTCTGCGAGAGCAACCAGGCACGGAT
CGACTACCGCAATCGCGAGGAAGGCGGCGGCTGCTTCCGCATCACCTTCGCCCACCCGCGCAAACTCAGCTGA

Domains

Predicted by InterproScan.

• Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c) (417-523)

• His Kinase A (phospho-acceptor) domain (HisKA) (310-373)

3D structure

• AlphaFold DB: Q51530

References

• [1]: J M Boyd et al. (1994) Identification and characterization of PilS, an essential regulator of pilin expression in Pseudomonas aeruginosa. Molecular & General Genetics : MGG 243(5):565-74. [PMID: 7911557]
• [2]: M Hobbs et al. (1993) PilS and PilR, a two-component transcriptional regulatory system controlling expression of type 4 fimbriae in Pseudomonas aeruginosa. Molecular Microbiology 7(5):669-82. [PMID: 8097014]