Detailed information    

experimental Experimentally validated

Overview

Name   pilV   Type   Machinery gene
Locus tag   PAKAF_RS24020 Genome accession   NZ_LR657304
Coordinates   5207460..5208017 (+) Length   185 a.a.
NCBI ID   WP_016253895.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa PAK     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

P. aeruginosa in biofilms is competent for natural transformation of both genomic and plasmid DNA. All T4P mutants were capable of some natural transformation of pUCPSK, however a significant reduction in transformation frequency compared to wild-type PAK was observed.


Genomic Context

Location: 5202460..5213017
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PAKAF_RS24000 (PAKAF_04822) pilR 5203736..5205073 (+) 1338 WP_003094694.1 two-component system response regulator PilR Regulator
  PAKAF_RS24005 thiO 5205119..5206213 (-) 1095 WP_016253894.1 glycine oxidase ThiO -
  PAKAF_RS24010 (PAKAF_04824) - 5206348..5206857 (+) 510 WP_003161431.1 Tfp pilus assembly protein FimT/FimU -
  PAKAF_RS24015 (PAKAF_04825) - 5206963..5207469 (+) 507 WP_003102598.1 Tfp pilus assembly protein FimT/FimU -
  PAKAF_RS24020 (PAKAF_04826) pilV 5207460..5208017 (+) 558 WP_016253895.1 type 4a pilus minor pilin PilV Machinery gene
  PAKAF_RS24025 (PAKAF_04827) pilW 5208014..5208838 (+) 825 WP_016253896.1 type 4a pilus minor pilin PilW -
  PAKAF_RS24030 (PAKAF_04828) pilX 5208835..5209422 (+) 588 WP_015648054.1 type 4a pilus minor pilin PilX -
  PAKAF_RS24035 (PAKAF_04829) pilY1 5209434..5212925 (+) 3492 WP_010793785.1 type 4a pilus biogenesis protein PilY1 -

Sequence

Protein


Download         Length: 185 a.a.        Molecular weight: 20064.17 Da        Isoelectric Point: 7.9350

>NTDB_id=1195 PAKAF_RS24020 WP_016253895.1 5207460..5208017(+) (pilV) [Pseudomonas aeruginosa PAK]
MLLKSRHRSLHQSGFSMIEVLVALLLISIGVLGMIAMQGKTIQYTADSVERNKAAMLGSNLLESMRASPKALYDVKDQMA
TQSDFFKAKGSAFPTAPSSCTPLPDAIKDRLGCWAEQVKNELPGAGDLLMSDYYICRSSKPGDCDGKGSMLEIRLAWRGK
QGACVNAADSSADTSLCYYTLRVEP

Nucleotide


Download         Length: 558 bp        

>NTDB_id=1195 PAKAF_RS24020 WP_016253895.1 5207460..5208017(+) (pilV) [Pseudomonas aeruginosa PAK]
ATGCTATTGAAATCGCGACACAGGTCGCTGCACCAATCCGGCTTCAGCATGATCGAAGTGCTGGTCGCCCTGCTGCTGAT
CAGCATCGGCGTGCTGGGCATGATCGCCATGCAGGGCAAGACCATCCAGTACACCGCCGACTCGGTCGAGCGCAACAAGG
CAGCCATGCTCGGCAGCAACCTGCTGGAAAGCATGCGGGCGAGCCCGAAGGCGCTGTACGACGTCAAGGACCAGATGGCC
ACGCAATCCGACTTCTTCAAGGCCAAGGGGTCGGCGTTCCCCACCGCGCCGTCCTCCTGCACGCCATTGCCCGACGCGAT
CAAGGACCGCCTGGGATGCTGGGCGGAACAGGTGAAGAACGAACTGCCAGGCGCTGGCGACCTGCTGATGAGCGACTACT
ACATCTGCCGCAGCTCGAAGCCGGGCGACTGCGACGGCAAGGGCTCCATGCTGGAAATCCGCCTGGCCTGGCGCGGCAAG
CAAGGCGCCTGCGTCAACGCCGCCGACAGCAGCGCCGACACCTCCCTCTGCTACTACACCCTCAGGGTTGAGCCATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(11-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Laura M Nolan et al. (2020) Pseudomonas aeruginosa is capable of natural transformation in biofilms. Microbiology (Reading, England) 166(10):995-1003. [PMID: 32749953]