Detailed information    

experimental Experimentally validated

Overview

Name   pilR   Type   Regulator
Locus tag   H0N27_RS16330 Genome accession   NZ_CP059039
Coordinates   3495106..3496527 (+) Length   473 a.a.
NCBI ID   WP_168727019.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain A118     
Function   regulation of type IV pilus assembly   
Competence regulation

Function

In contrast to A. baylyi, deletion of pilS or pilR as well as chpA or pilG in A. baumannii completely abolished transformation, while the absence of pilH did not change the strain’s transformation efficiency


Genomic Context

Location: 3490106..3501527
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H0N27_RS16310 (H0N27_16265) thrC 3490362..3491501 (+) 1140 WP_000063593.1 threonine synthase -
  H0N27_RS16315 (H0N27_16270) pbpG 3491608..3492654 (-) 1047 WP_001984577.1 D-alanyl-D-alanine endopeptidase PBP7/8 -
  H0N27_RS16320 (H0N27_16275) - 3492867..3493502 (+) 636 WP_000633799.1 response regulator -
  H0N27_RS16325 (H0N27_16280) pilS 3493513..3495081 (+) 1569 WP_206677389.1 sensor histidine kinase Regulator
  H0N27_RS16330 (H0N27_16285) pilR 3495106..3496527 (+) 1422 WP_168727019.1 sigma-54-dependent transcriptional regulator Regulator
  H0N27_RS16335 (H0N27_16290) - 3496531..3497715 (-) 1185 WP_000939107.1 S41 family peptidase -
  H0N27_RS16340 (H0N27_16295) gpmI 3497731..3499278 (-) 1548 WP_031963978.1 2,3-bisphosphoglycerate-independent phosphoglycerate mutase -
  H0N27_RS16345 (H0N27_16300) lptG 3499404..3500474 (-) 1071 WP_000881094.1 LPS export ABC transporter permease LptG -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  pilR pilA positive effect
  hns pilA negative effect
  hns pilQ negative effect
  hns pilT negative effect
  hns comA/comEC negative effect
  hns comF negative effect
  hns dprA negative effect
  hns comEA negative effect
  pilS pilR positive effect

Sequence

Protein


Download         Length: 473 a.a.        Molecular weight: 53022.16 Da        Isoelectric Point: 6.6690

>NTDB_id=1045 H0N27_RS16330 WP_168727019.1 3495106..3496527(+) (pilR) [Acinetobacter baumannii strain A118]
MAEQQPLVLLVDDEEDLCLLMQMTLARMGIKTHLAYRVEQAKQLFTQFHYDACLTDLNLPDGSGLDLVKHVSQNYPNTPI
AVLTAYGNMDIAIAALKAGAFDFVSKPVNQVHLDQLLQKALNRQKVEHDTTENALENKLLIGRSLPIQQLRIAIKKIARS
QAPVFVTGESGTGKEVVANLVHRLSNRSEGPFIAINCGAIPTELMESELFGHKKGSFTGATQDKQGLILSAHGGSLFLDE
IAELPLSMQVKLLRAVQEKKIRPVGSDQEIDVDFRVISASHQDLDLLVRQGKFRQDLFFRIHVMDLILPPLRERGEDVLL
LANHFIQKICMEWETPPKQLTEAAETYLLQQHFPGNVRELRNMIERAITLSEDATIDISHLHPAPLRANISNPFASAAQS
IQTTVAAPQAVKKLPSEGLERYLENIEKDILLNALNMTHWNRTLAAKKLGMTFRSLRYRLKKFGLDTETEQEV

Nucleotide


Download         Length: 1422 bp        

>NTDB_id=1045 H0N27_RS16330 WP_168727019.1 3495106..3496527(+) (pilR) [Acinetobacter baumannii strain A118]
ATGGCAGAACAGCAACCACTGGTTTTGCTTGTAGACGATGAAGAAGATTTGTGCCTTTTAATGCAAATGACACTTGCACG
AATGGGGATTAAAACACATCTTGCTTATCGGGTTGAACAGGCCAAACAACTCTTCACACAGTTTCATTACGATGCATGTT
TAACCGACTTAAACCTACCCGATGGGAGTGGGTTAGACTTAGTCAAACATGTCTCTCAAAATTATCCCAACACTCCGATT
GCGGTTTTAACTGCTTACGGCAATATGGATATTGCAATTGCAGCATTAAAAGCAGGCGCTTTTGATTTTGTAAGCAAACC
GGTCAACCAAGTACATCTAGATCAATTATTACAAAAAGCCTTGAATCGGCAAAAAGTAGAGCATGATACTACCGAGAATG
CGTTAGAAAATAAATTATTAATCGGCCGTTCTCTACCCATCCAGCAGCTACGTATTGCAATTAAAAAAATTGCGCGCTCA
CAAGCACCTGTATTTGTTACCGGTGAGTCTGGAACAGGTAAAGAAGTAGTTGCCAATTTGGTTCATCGGCTGAGTAACCG
TAGTGAAGGCCCTTTTATTGCAATTAACTGCGGTGCCATTCCAACCGAACTCATGGAAAGTGAGCTTTTTGGGCATAAAA
AAGGTAGCTTTACCGGAGCAACTCAAGATAAACAAGGCCTCATTTTATCGGCACACGGCGGTAGTCTATTTTTAGATGAA
ATTGCCGAATTACCTTTAAGTATGCAGGTCAAACTGCTCCGTGCAGTACAGGAAAAAAAGATCCGACCCGTCGGTTCAGA
TCAGGAAATCGATGTTGATTTCCGTGTGATTAGTGCAAGTCATCAAGACTTAGATTTATTGGTTAGACAAGGTAAATTCC
GTCAAGATTTATTCTTCCGTATTCACGTTATGGACCTCATATTGCCACCTCTACGTGAACGTGGCGAAGATGTCCTTTTA
CTGGCCAATCACTTTATTCAGAAAATTTGTATGGAGTGGGAAACGCCACCTAAACAATTAACAGAAGCAGCCGAAACTTA
TCTGCTACAGCAACACTTTCCGGGTAATGTTCGCGAATTAAGAAATATGATTGAGCGCGCAATTACCTTAAGTGAAGATG
CCACCATAGATATATCTCATTTACATCCTGCTCCTCTAAGAGCGAATATTTCTAATCCTTTTGCTTCGGCTGCTCAAAGC
ATACAAACCACTGTGGCAGCTCCTCAAGCCGTAAAAAAATTACCAAGTGAAGGTCTAGAACGTTATTTAGAAAATATTGA
AAAAGATATTTTACTCAATGCACTCAATATGACTCATTGGAATCGTACCTTAGCAGCTAAAAAATTAGGAATGACTTTCC
GCTCTTTACGCTATCGTCTGAAAAAATTTGGCTTAGATACTGAGACAGAACAGGAAGTATAA

Domains

Predicted by InterproScan.

Bacterial regulatory protein, Fis family (HTH_8)

(423-463)

Response regulator receiver domain (Response_reg)

(8-117)

Sigma-54 interaction domain (Sigma54_activat)

(140-306)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

51.198

100

0.528

Multiple sequence alignment    



References

[1] Nina Vesel et al. (2021) Pilus Production in Acinetobacter baumannii Is Growth Phase Dependent and Essential for Natural Transformation. Journal of Bacteriology 203(8):e00034-21. [PMID: 33495250]