Detailed information    

experimental Experimentally validated

Overview

Name   pilT   Type   Machinery gene
Locus tag   H0N27_RS13360 Genome accession   NZ_CP059039
Coordinates   2870674..2871711 (+) Length   345 a.a.
NCBI ID   WP_000355489.1    Uniprot ID   A0A009PTR1
Organism   Acinetobacter baumannii strain A118     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

Mutant studies with A. baumannii A118 confirmed that pilA, pilQ (secretin, comQ homologue), pilT (retraction ATPase), comEA (DNA-binding protein), comF (pilotin) and dprA (DNA protecting protein) are required for natural transformation


Genomic Context

Location: 2865674..2876711
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H0N27_RS13350 (H0N27_13350) - 2866058..2869654 (+) 3597 WP_168726752.1 AAA family ATPase -
  H0N27_RS13355 (H0N27_13355) - 2869855..2870547 (-) 693 WP_168726751.1 YggS family pyridoxal phosphate-dependent enzyme -
  H0N27_RS13360 (H0N27_13360) pilT 2870674..2871711 (+) 1038 WP_000355489.1 type IV pilus twitching motility protein PilT Machinery gene
  H0N27_RS13365 (H0N27_13365) pilU 2871739..2872857 (+) 1119 WP_000347034.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H0N27_RS13370 (H0N27_13370) fur 2872927..2873364 (-) 438 WP_001122847.1 ferric iron uptake transcriptional regulator -
  H0N27_RS13375 (H0N27_13375) - 2873476..2873874 (+) 399 WP_001170994.1 outer membrane protein assembly factor BamE -
  H0N27_RS13380 (H0N27_13380) - 2873929..2874252 (-) 324 WP_045901052.1 RnfH family protein -
  H0N27_RS13385 (H0N27_13385) - 2874252..2875319 (-) 1068 WP_168726750.1 hypothetical protein -
  H0N27_RS13390 (H0N27_13390) - 2875472..2875927 (-) 456 WP_000782976.1 bacteriohemerythrin -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  hns pilT negative effect
  hns pilQ negative effect
  hns comA/comEC negative effect
  hns comF negative effect
  hns pilA negative effect
  pilR pilA positive effect
  hns dprA negative effect
  hns comEA negative effect
  pilS pilR positive effect

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38312.92 Da        Isoelectric Point: 6.7271

>NTDB_id=1040 H0N27_RS13360 WP_000355489.1 2870674..2871711(+) (pilT) [Acinetobacter baumannii strain A118]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMLDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANHGMTTLDQSL
KGLVARGVISPQTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1040 H0N27_RS13360 WP_000355489.1 2870674..2871711(+) (pilT) [Acinetobacter baumannii strain A118]
ATGGATATTACAGAGCTACTCGCCTTCTCTGTGAAAAATGGCGCGTCCGATTTACATCTCTCTGCTGGCATGCCACCAAT
GATTCGTGTTGACGGTGAAGTTCGTCGTATTAACTTACCAGCCTTAGAACATAAAGATGTCCACCGTCTTGTTTACGACA
TCATGAATGATAAACAGCGCCGTGACTATGAAGAAAAACTCGAAACTGACTTTTCTTTTGAAGTACCTAATGTTGCGCGT
TTCCGTGTTAACGCATTTAACCAAAACCGTGGTGCTGGTGCAGTATTTCGTACCATTCCATCTAAAGTCCTCACCATGGA
AGATCTAGGTCTAGGACAAATCTTTAAAGATATTTGCGACTATCCTCGTGGCATTGTACTGGTGACAGGACCAACAGGTT
CGGGTAAATCAACTACGCTTGCAGCGATGCTCGATTATATTAACGAAAACCGCTATGACCATATTTTAACGGTGGAAGAC
CCAATCGAATTTGTACACCAGTCTAAGAAATGTCTCATTAACCAGCGTGAAGTGCATCGTGACACACACGGCTTTAATGA
AGCCCTTCGCTCAGCACTACGTGAAGACCCAGATATTATTTTAGTCGGTGAGATGCGTGACCTTGAAACCATTCGCTTAG
CATTAACTGCTGCCGAAACAGGTCACTTGGTGTTTGGTACGCTCCACACCACGTCGGCAGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCCGAAGAGAAAGACATGGTTCGTGCCATGCTGTCTGAATCTTTACAAGCGGTTATTTCACAAAC
CCTGCTTAAAAAGAATGGCGGTGGCCGCGTCGCAGCTCATGAAATCATGATTGGTATTCCGGCTATTCGTAACTTAATTC
GTGAAAACAAAGTCGCGCAAATGTACTCAGCCATTCAAACTGGTGCCAATCATGGCATGACCACACTAGACCAAAGCTTA
AAAGGCTTAGTCGCTCGCGGTGTGATTAGTCCACAAACCGCACGTACTGCGGCAAAACAGCCTGAATCATTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A009PTR1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

100

100

1
  pilT Acinetobacter nosocomialis M2

99.71

100

0.997
  pilT Acinetobacter baylyi ADP1

88.953

100

0.89
  pilT Pseudomonas aeruginosa PAK

84.302

100

0.843
  pilT Pseudomonas stutzeri DSM 10701

84.012

100

0.84
  pilT Legionella pneumophila strain ERS1305867

75.581

100

0.756
  pilT Legionella pneumophila strain Lp02

75.581

100

0.756
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.726

97.391

0.699
  pilT Vibrio cholerae strain A1552

71.726

97.391

0.699
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.522
  pilU Vibrio cholerae strain A1552

41.593

98.261

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.101

0.394
  pilU Acinetobacter baylyi ADP1

38.728

100

0.388

Multiple sequence alignment    



References

[1] Nina Vesel et al. (2021) Pilus Production in Acinetobacter baumannii Is Growth Phase Dependent and Essential for Natural Transformation. Journal of Bacteriology 203(8):e00034-21. [PMID: 33495250]
[2] Casin Le et al. (2021) Involvement of the Histone-Like Nucleoid Structuring Protein (H-NS) in Acinetobacter baumannii's Natural Transformation. Pathogens (Basel, Switzerland) 10(9):1083. [PMID: 34578115]