Detailed information    

experimental Experimentally validated

Overview

Name   pilT   Type   Machinery gene
Locus tag   NGFG_RS10615 Genome accession   NC_022240
Coordinates   1951884..1952927 (+) Length   347 a.a.
NCBI ID   WP_003690200.1    Uniprot ID   Q53432
Organism   Neisseria gonorrhoeae MS11     
Function   pseudopilus biogenesis   
DNA binding and uptake

Function

The pilus retraction protein PilT is largely dispensable for functional DNA binding and thus acts specifically in DNA uptake. Piliated PilT mutants and a panel of pilus assembly mutants were found to be deficient in sequence-specific DNA uptake into the cell, the earliest demonstrable step in neisserial competence.


Genomic Context

Location: 1946884..1957927
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGFG_RS10595 (NGFG_01984) dksA 1949267..1949683 (-) 417 WP_003692108.1 RNA polymerase-binding protein DksA -
  NGFG_RS10600 (NGFG_01983) proC 1949831..1950622 (-) 792 WP_003696395.1 pyrroline-5-carboxylate reductase -
  NGFG_RS10605 (NGFG_01982) - 1950671..1951078 (-) 408 WP_003690202.1 hypothetical protein -
  NGFG_RS10610 (NGFG_01981) - 1951078..1951764 (-) 687 WP_003690201.1 YggS family pyridoxal phosphate-dependent enzyme -
  NGFG_RS10615 (NGFG_01980) pilT 1951884..1952927 (+) 1044 WP_003690200.1 type IV pilus twitching motility protein PilT Machinery gene
  NGFG_RS10620 (NGFG_01979) - 1953091..1954317 (+) 1227 WP_003690199.1 PilT/PilU family type 4a pilus ATPase -
  NGFG_RS10625 (NGFG_01978) yccS 1954434..1956584 (+) 2151 WP_003694501.1 YccS family putative transporter -
  NGFG_RS10630 (NGFG_02247) - 1957514..1957798 (-) 285 WP_003692093.1 GIY-YIG nuclease family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 37940.31 Da        Isoelectric Point: 6.8962

>NTDB_id=1116 NGFG_RS10615 WP_003690200.1 1951884..1952927(+) (pilT) [Neisseria gonorrhoeae MS11]
MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSAEEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVAR
FRVNAFNTGRGPAAVFRTIPSTVLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
PIEFVHQSKKSLINQRELHQHTLSFANALSSALREDPDVILVGEMRDPETIGLALTAAETGHLVFGTLHTTGAAKTVDRI
VDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNGRVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQS
LQSLVRQGLIAPEAARRRAQNSESMSF

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1116 NGFG_RS10615 WP_003690200.1 1951884..1952927(+) (pilT) [Neisseria gonorrhoeae MS11]
ATGCAGATTACCGACTTACTCGCCTTCGGCGCTAAAAACAAAGCATCCGACCTTCACCTGAGTTCGGGCATATCCCCTAT
GATTCGGGTTCACGGCGACATGCGGCGCATCAACCTTCCCGAAATGAGCGCGGAAGAGGTCGGCAATATGGTAACTTCGG
TGATGAACGACCACCAGCGGAAAATCTACCAGCAAAACTTGGAAGTCGACTTCTCGTTCGAACTGCCCAACGTCGCCCGA
TTCCGCGTCAACGCCTTCAACACCGGCCGCGGCCCCGCCGCCGTATTCCGCACCATTCCCAGCACCGTCTTATCGCTGGA
AGAATTGAAAGCCCCGAGCATTTTCCAAAAAATCGCAGAATCGCCGCGCGGCATGGTATTGGTTACCGGCCCTACCGGTT
CGGGCAAATCGACCACGCTTGCCGCGATGATCAACTACATCAACGAAACCCAGCCGGCACACATCCTGACCATCGAAGAC
CCGATCGAATTCGTCCACCAAAGCAAAAAATCCCTGATTAACCAACGCGAGCTGCACCAGCACACCCTCAGCTTCGCCAA
CGCGCTGAGTTCCGCATTGCGCGAAGACCCAGACGTTATCCTTGTCGGCGAGATGCGCGACCCCGAAACCATCGGCTTGG
CACTGACCGCCGCCGAAACCGGACACTTGGTTTTCGGCACGCTGCACACGACCGGCGCGGCAAAAACCGTCGACCGTATC
GTGGACGTATTCCCCGCCGGAGAGAAAGAAATGGTGCGTTCCATGCTGTCCGAATCGCTGACCGCCGTCATCTCCCAAAA
CCTGCTGAAAACGCACGACGGCAACGGCCGTGTCGCCTCGCACGAAATCCTGATTGCCAACCCCGCCGTCCGCAACCTCA
TCCGCGAAAACAAAATCACGCAGATTAACTCCGTCCTGCAAACCGGGCAGGCGAGCGGTATGCAGACGATGGACCAATCG
CTGCAATCGCTGGTGCGCCAAGGGCTGATCGCACCGGAAGCCGCACGCAGACGCGCGCAAAACAGCGAAAGTATGAGTTT
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q53432

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

99.135

100

0.991
  pilT Acinetobacter baumannii strain A118

67.826

100

0.678
  pilT Acinetobacter nosocomialis M2

67.826

100

0.678
  pilT Acinetobacter baumannii D1279779

67.826

100

0.678
  pilT Acinetobacter baylyi ADP1

67.246

100

0.674
  pilT Pseudomonas stutzeri DSM 10701

66.957

100

0.672
  pilT Pseudomonas aeruginosa PAK

66.377

100

0.666
  pilT Legionella pneumophila strain ERS1305867

64.516

99.128

0.64
  pilT Legionella pneumophila strain Lp02

64.516

99.128

0.64
  pilT Vibrio cholerae strain A1552

64.242

95.652

0.614
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.242

95.652

0.614
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.392

97.695

0.522
  pilU Pseudomonas stutzeri DSM 10701

40.29

99.424

0.401
  pilU Vibrio cholerae strain A1552

38.329

100

0.383
  pilU Acinetobacter baylyi ADP1

38.439

99.712

0.383

Multiple sequence alignment    



References

[1] Berenike Maier et al. (2004) A force-dependent switch reverses type IV pilus retraction. Proceedings of The National Academy of Sciences of The United States of America 101(30):10961-6. [PMID: 15256598]
[2] Finn Erik Aas et al. (2002) Competence for natural transformation in Neisseria gonorrhoeae: components of DNA binding and uptake linked to type IV pilus expression. Molecular Microbiology 46(3):749-60. [PMID: 12410832]
[3] M Wolfgang et al. (1998) PilT mutations lead to simultaneous defects in competence for natural transformation and twitching motility in piliated Neisseria gonorrhoeae. Molecular Microbiology 29(1):321-30. [PMID: 9701824]
[4] M Wolfgang et al. (1998) Suppression of an absolute defect in type IV pilus biogenesis by loss-of-function mutations in pilT, a twitching motility gene in Neisseria gonorrhoeae. Proceedings of The National Academy of Sciences of The United States of America 95(25):14973-8. [PMID: 9844000]